Home Cytoskeletal Signaling Microtubule Associated inhibitor iHAP1 For research use only.

iHAP1

iHAP1 is a well-established microtubule poison, blocks microtubule polymerization fully at 5 µM which is independent of deregulated transcription, causes marked sensitization when deleting Rod1 gene in HeLa cells.

iHAP1 Chemical Structure

iHAP1 Chemical Structure

CAS No. 105925-39-1

Purity & Quality Control

Batch: S969501 DMSO]20 mg/mL]false]Ethanol]5 mg/mL]false]Water]Insoluble]false Purity: 99.84%
99.84

iHAP1 Related Products

Signaling Pathway

Microtubule Associated Signaling Pathways

Microtubule Associated Signaling Pathway

Biological Activity

Description iHAP1 is a well-established microtubule poison, blocks microtubule polymerization fully at 5 µM which is independent of deregulated transcription, causes marked sensitization when deleting Rod1 gene in HeLa cells.
Targets
microtubule polymerization [1]
In vitro
In vitro

The depletion of Rod1 and TACC3 results in sensitization to iHAP1, which is caused by chemogenic interaction between Rod1 and iHAP1. As for the RPE1‐hTERT P53−/− background, deletion of Rod1 in HeLa cells causes marked sensitization to iHAP1.[1]
Cell Research Cell lines RPE1‐hTERT P53−/− Flag‐Cas9 cell line
Concentrations 0.16-1 µM
Incubation Time 12 days
Method

The Sulforhodamine B (SRB) proliferation assay was used to assess in vitro cytotoxicity of the compounds. Cells were treated with sublethal drug concentrations (LD20) for 12 days from the day of seeding. If necessary, transient siRNA transfection was performed 3 days before drug treatment. After 12 days, medium was removed from the wells and cells were washed once with PBS and fixed in prechilled 10% trichloroacetic acid (TCA) for 30 min at 4°C. Cells were subsequently washed two times with double‐distilled water and stained with 0.4% SRB (1% acetic acid for 20 min at room temperature protected from light). SRB was then removed, and cells were washed four times in 1% acetic acid. After complete light‐protected drying, SRB was dissolved by addition of 10 mM Tris pH 8 and gentle shaking at room temperature for 2 h. The absorbance at A510 was read. Percentage of cell growth inhibition was calculated. The IncuCyte® live‐cell analysis system was additionally used to real‐time track the growth of the cells subjected to the different treatments.

Chemical Information & Solubility

Molecular Weight 367.85 Formula

C20H14ClNO2S
CAS No. 105925-39-1 SDF --
Smiles COC1=CC=C(C=C1)C(=O)N2C3=CC=CC=C3SC4=C2C=C(Cl)C=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 20 mg/mL ( (54.36 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy iHAP1 | iHAP1 supplier | purchase iHAP1 | iHAP1 cost | iHAP1 manufacturer | order iHAP1 | iHAP1 distributor