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Hemin

Synonyms: protohemin

Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.Hemin is an Heme oxygenase (HO)-1 inducer.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!

Hemin Chemical Structure

Hemin Chemical Structure

CAS No. 16009-13-5

Purity & Quality Control

Hemin Related Products

Biological Activity

Description Hemin (protohemin) is an iron-containing porphyrin used in the management of porphyria attacks, particularly in acute intermittent porphyria.Hemin is an Heme oxygenase (HO)-1 inducer.This product has poor solubility, animal experiments are available, cell experiments please choose carefully!
In vitro
In vitro

Treatment of hemin or AFC damped the expression of TFRC in CT26 cells, which can be significantly rescued by overexpression of OTUD1.[1]
Cell Research Cell lines CT26 cells
Concentrations 100 μM
Incubation Time 30 min
Method

Mock‐ and OTUD1‐expressing CT26 cells are treated with hemin (100 μM). Flow cytometric analyses the TFRC expression.
In Vivo
In vivo

Following the i.p. administration of Hemin (100 μM/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03338816 Completed
Acute Hepatic Porphyria|Acute Intermittent Porphyria|Porphyria Acute Intermittent|Acute Porphyria|Hereditary Coproporphyria (HCP)|Variegate Porphyria (VP)|ALA Dehydratase Deficient Porphyria (ADP)
Alnylam Pharmaceuticals
 November 16 2017 Phase 3
NCT02949830 Completed
Acute Intermittent Porphyria
Alnylam Pharmaceuticals
 October 2016 Phase 1|Phase 2
NCT01855841 Completed
Post-ERCP Acute Pancreatitis
Erasme University Hospital
 April 2012 Phase 2

Chemical Information & Solubility

Molecular Weight 651.94 Formula

C34H32ClFeN4O4
CAS No. 16009-13-5 SDF --
Smiles CC1=C(C2=CC3=C(C(=C([N-]3)C=C4C(=C(C(=N4)C=C5C(=C(C(=N5)C=C1[N-]2)C)C=C)C)C=C)C)CCC(=O)O)CCC(=O)O.[Cl-].[Fe+3]
Storage (From the date of receipt) 3 years -20°C(in the dark) powder

In vitro
Batch:

DMSO : 20 mg/mL ( (30.67 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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