Home Immunology & Inflammation NOS inhibitor - COX inhibitor - NF-κB inhibitor Hederagenin For research use only.

Hederagenin

Synonyms: Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E

Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. Hederagenin inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.

Hederagenin Chemical Structure

Hederagenin Chemical Structure

CAS No. 465-99-6

Purity & Quality Control

Batch: S389901 DMSO]94 mg/mL]false]]]false]]]false Purity: 98%
98

Hederagenin Related Products

Biological Activity

Description Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. Hederagenin inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.
Targets
iNOS [2] COX-2 [2] NF-κB [2]
In vitro
In vitro Hederagenin could induce apoptosis of LoVo cells through the mitochondrial apoptotic pathway. Hederagenin could induce the up-regulation of Bax and down-regulation of Bcl-2, Bcl-xL and Survivin, the release of cytochrome c, followed by procaspase-9, procaspase-3 and proPARP cleavage[1]. Hederagenin exhibits antiinflammatory activities via decreasing production of pro-inflammatory mediators (NO, PGE2, TNF-α, IL-1β, and IL-6) through suppression of the pathways of NF-κB in LPS-induced RAW264.7 cells[2].
Cell Research Cell lines LoVo cells
Concentrations 0.25, 0.5, 1.0, 2.0 and 4.0 μM
Incubation Time 24 or 48 h
Method LoVo cells are seeded into 96-well plates at a density of 10,000 cells/well. After being treated with 0.25, 0.5, 1.0, 2.0 and 4.0 μM hederagenin for 24 or 48 h, the medium is discarded and treated with 20 μl MTT (5 mg/ml). After incubation for 4 h at 37°C, the 100 μl DMSO is added to each well to dissolve the resultant formazan crystals. Absorbance is detected at 490 nm.

Chemical Information & Solubility

Molecular Weight 472.70 Formula

C30H48O4
CAS No. 465-99-6 SDF --
Smiles CC1(CCC2(CCC3(C(=CCC4C3(CCC5C4(CCC(C5(C)CO)O)C)C)C2C1)C)C(=O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 94 mg/mL ( (198.85 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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