Home Immunology & Inflammation Antioxidant chemical Gipsoside For research use only.

Gipsoside

Gipsoside is a triterpene saponin from gypsophila paniculata L.

Gipsoside Chemical Structure

Gipsoside Chemical Structure

CAS No. 15588-68-8

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Gipsoside Related Products

Biological Activity

Description Gipsoside is a triterpene saponin from gypsophila paniculata L.
In vitro
In vitro

Gipsoside has an activity of anti-inflammatory, anti-thrombotic, antioxidative and anti-cancer actions. Gypenoside inhibited SW-480 cell proliferation in a dose- and time-dependent manner. Gyp is capable of exerting different alternative cytotoxicity in cancer cells and normal cells, which might be potentially useful as a cancer preventive or treatment agent. Gyp could cause cell membrane integrity damage, decrease the Δψm level, induce DNA fragmentation and initiate apoptotic response in SW-480 cells. ROS generated in SW-480 cells play an important role in Gyp induced cell death. Gyp induces microfilament network collapse and injures the cell shape and migration ability[1]. It is reported that Gypenoside can induce neuroprotection against Aβ in vitro. Gypenoside attenuates Aβ-induced microglial activation, decreases the levels of microglial M1 state (classic activated state) markers, including iNOS protein expression, TNF-α, IL-1β, and IL-6 releases, and increases the levels of M2 markers, such as Arg-1 protein expression, IL-10, BDNF, and GDNF secretions from the cells. Gypenoside reduces the Aβ-induced microglial activation by shifting microglial M1 to M2 (alternative activated state) state, and the SOCS1 protein may mediate the process[2].
Cell Research Cell lines SW-480 cells
Concentrations 0, 70, 100 and 130 µg/ml
Incubation Time 24 and 48 h
Method

To investigate the effect of Gypenoside on SW-480 cell proliferation, cells are seeded in 96-well plates. Various concentrations (0, 70, 100 and 130 µg/ml; 80% ethanol is used as the solvent control) of Gypenoside are added and the cells are incubated for various periods of time, at a density of 1×105 cells/ml, respectively. The cell viability is determined by using MTT assay. The absorbance at 570 nm is recorded using a microplate reader.
In Vivo
In vivo

Gipsoside has been known for its wide beneficial effects for treating hepatitis, hyperlipoproteinemia and cardiovascular disease[1].

Chemical Information & Solubility

Molecular Weight 1791.83 Formula

C80H126O44
CAS No. 15588-68-8 SDF Download Gipsoside SDF
Storage (From the date of receipt) 3 years -20°C powder (seal)

In vitro
Batch:

DMSO : 100 mg/mL ( (55.8 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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