Gipsoside

Catalog No.S5151 Batch:S515101

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Technical Data

Formula

C80H126O44

Molecular Weight 1791.83 CAS No. 15588-68-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (55.8 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Gipsoside is a triterpene saponin from gypsophila paniculata L.
In vitro

Gipsoside has an activity of anti-inflammatory, anti-thrombotic, antioxidative and anti-cancer actions. Gypenoside inhibited SW-480 cell proliferation in a dose- and time-dependent manner. Gyp is capable of exerting different alternative cytotoxicity in cancer cells and normal cells, which might be potentially useful as a cancer preventive or treatment agent. Gyp could cause cell membrane integrity damage, decrease the Δψm level, induce DNA fragmentation and initiate apoptotic response in SW-480 cells. ROS generated in SW-480 cells play an important role in Gyp induced cell death. Gyp induces microfilament network collapse and injures the cell shape and migration ability[1]. It is reported that Gypenoside can induce neuroprotection against Aβ in vitro. Gypenoside attenuates Aβ-induced microglial activation, decreases the levels of microglial M1 state (classic activated state) markers, including iNOS protein expression, TNF-α, IL-1β, and IL-6 releases, and increases the levels of M2 markers, such as Arg-1 protein expression, IL-10, BDNF, and GDNF secretions from the cells. Gypenoside reduces the Aβ-induced microglial activation by shifting microglial M1 to M2 (alternative activated state) state, and the SOCS1 protein may mediate the process[2].

In vivo

Gipsoside has been known for its wide beneficial effects for treating hepatitis, hyperlipoproteinemia and cardiovascular disease[1].

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    SW-480 cells

  • Concentrations

    0, 70, 100 and 130 µg/ml

  • Incubation Time

    24 and 48 h

  • Method

    To investigate the effect of Gypenoside on SW-480 cell proliferation, cells are seeded in 96-well plates. Various concentrations (0, 70, 100 and 130 µg/ml; 80% ethanol is used as the solvent control) of Gypenoside are added and the cells are incubated for various periods of time, at a density of 1×105 cells/ml, respectively. The cell viability is determined by using MTT assay. The absorbance at 570 nm is recorded using a microplate reader.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.