Home DNA Damage/DNA Repair FENs inhibitor FEN1-IN-4 For research use only.

FEN1-IN-4

Synonyms: FEN1 Inhibitor C2, JUN93587

FEN1-IN-4 (FEN1 Inhibitor C2, JUN93587, Compound 2) is an inhibitor of human flap endonuclease-1 (hFEN1) with IC50 of 30 nM for hFEN1-336Δ.

FEN1-IN-4 Chemical Structure

FEN1-IN-4 Chemical Structure

CAS No. 1995893-58-7

Purity & Quality Control

FEN1-IN-4 Related Products

Biological Activity

Description FEN1-IN-4 (FEN1 Inhibitor C2, JUN93587, Compound 2) is an inhibitor of human flap endonuclease-1 (hFEN1) with IC50 of 30 nM for hFEN1-336Δ.
Targets
hFEN1-336Δ [1]
(Cell-free assay)
30 nM
In vitro
In vitro

FEN1-IN-4 (FEN1 Inhibitor C2, JUN93587, Compound 2) is an inhibitor of human flap endonuclease-1 (hFEN1) that abrogated mtDNA fragmentation and cytosolic Ox-mtDNA and mtDNA release and inhibited alum-induced NLRP3 inflammasome-dependent IL-1β production in vivo and reduced neutrophil and monocyte infiltration, without an effect on TNF secretion.
Cell Research Cell lines BMDM
Concentrations 10 μM
Incubation Time 16 h
Method

Cells were treated with indicated concentration of drug for 16 h.

Chemical Information & Solubility

Molecular Weight 232.24 Formula
C12H12N2O3
CAS No. 1995893-58-7 SDF --
Smiles ON1C(=O)N(CC2CC2)C3=C(C=CC=C3)C1=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 46 mg/mL ( (198.07 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 46 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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