FEN1-IN-4

Catalog No.S3397 Batch:S339701

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Technical Data

Formula
C12H12N2O3
Molecular Weight 232.24 CAS No. 1995893-58-7
Solubility (25°C)* In vitro DMSO 46 mg/mL (198.07 mM)
Ethanol 46 mg/mL (198.07 mM)
Water Insoluble
In vivo (Add solvents to the product individually and in order)
5% DMSO 95% Corn oil
2.5mg/ml
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
2.5mg/ml
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description FEN1-IN-4 (FEN1 Inhibitor C2, JUN93587, Compound 2) is an inhibitor of human flap endonuclease-1 (hFEN1) with IC50 of 30 nM for hFEN1-336Δ.
Targets
hFEN1-336Δ [1]
(Cell-free assay)
30 nM
In vitro

FEN1-IN-4 (FEN1 Inhibitor C2, JUN93587, Compound 2) is an inhibitor of human flap endonuclease-1 (hFEN1) that abrogated mtDNA fragmentation and cytosolic Ox-mtDNA and mtDNA release and inhibited alum-induced NLRP3 inflammasome-dependent IL-1β production in vivo and reduced neutrophil and monocyte infiltration, without an effect on TNF secretion.

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    BMDM

  • Concentrations

    10 μM

  • Incubation Time

    16 h

  • Method

    Cells were treated with indicated concentration of drug for 16 h.

Selleck's FEN1-IN-4 has been cited by 3 publications

PARP1-driven repair of topoisomerase IIIα DNA-protein crosslinks by FEN1 [ Cell Rep, 2024, 43(8):114522] PubMed: 39028621
FEN1 Inhibition as a Potential Novel Targeted Therapy against Breast Cancer and the Prognostic Relevance of FEN1 [ Int J Mol Sci, 2024, 25(4)2110] PubMed: 38396787
Oxidized DNA fragments exit mitochondria via mPTP- and VDAC-dependent channels to activate NLRP3 inflammasome and interferon signaling [ Immunity, 2022, S1074-7613(22)00280-1] PubMed: 35835107

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.