Home DNA Damage/DNA Repair Telomerase activator Cycloastragenol For research use only.

Cycloastragenol

Synonyms: CAG, TA-65, Cyclogalegigenin, Astramembrangenin

Cycloastragenol (CAG, TA-65, Cyclogalegigenin, Astramembrangenin) is a saponin comprising a group of oil glucosides naturally present in a number of plants. It is a potent telomerase activator in neuronal cells.

Cycloastragenol Chemical Structure

Cycloastragenol Chemical Structure

CAS No. 84605-18-5

Purity & Quality Control

Cycloastragenol Related Products

Signaling Pathway

Telomerase Signaling Pathways

Telomerase Signaling Pathway

Biological Activity

Description Cycloastragenol (CAG, TA-65, Cyclogalegigenin, Astramembrangenin) is a saponin comprising a group of oil glucosides naturally present in a number of plants. It is a potent telomerase activator in neuronal cells.
Targets
telomerase [1]
In vitro
In vitro Cycloastragenol stimulates telomerase activity and cell proliferation in human neonatal keratinocytes. It induces telomerase activity and cAMP response element binding (CREB) activation in PC12 cells and primary neurons. CAG treatment not only induces the expression of bcl2, a CREB-regulated gene, but also the expression of telomerase reverse transcriptase in primary cortical neurons[1]. Cycloastragenol rapidly passes through the Caco-2 cell monolayer by passive diffusion, once passage through the intestinal epithelium, first-pass intestinal metabolism of Cycloastragenol might occur. Cycloastragenol can undergo extensive metabolism in rat and human liver microsomes[2].
Cell Research Cell lines HEKn cells, PC12 cells
Concentrations --
Incubation Time 24 h
Method HEKn cells (population doubling time: 3-6 days), PC12 cells (passage No.: 12-18), cultured primary cortical, and hippocampal neurons [12 days in vitro (DIV)] are treated with CAG for 24 h. Total cell lysates are then collected by using the lysis buffer provided in the real-time quantitative telomeric repeat amplification protocol (RQ-TRAP) assay kit. Telomerase activity is determined by using an ABI Prism 7000.
In Vivo
In vivo Oral administration of cycloastragenol (CA) for 7 days attenuates depression-like behavior in experimental mice. Oral bioavailability of cycloastragenol is about 25.70% at 10 mg/kg. Cycloastragenol is excreted through bile and feces and eliminated predominantly by the kidney in rats. It also might exist an enterohepatic circulation of cycloastragenol in rats. Cycloastragenol could be metabolized widely in vivo in rat. For oral administration the mean Tmax at 10 mg/kg, 20 mg/kg, and 40 mg/kg is 2.06±0.58 h, 1.48±0.36 h, and 2.35± 1.17 h. The t1/2 is 5.23±1.55 h, 7.33±3.03 h, and 6.06± 3.42 h, respectively. The mean absorption time of CA at 10 mg/kg is 5.70± 1.62 h; the poor absorption could be caused by the low solubility. The pharmacokinetic parameters show no significant differences among the groups of 10, 20, and 40 mg/kg except for Cmax and AUC[2].
Animal Research Animal Models male ICR mice
Dosages 100 mg/kg
Administration by oral gavage
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05777083 Recruiting
ST Elevation Myocardial Infarction|Electrocardiogram
Ewha Womans University Mokdong Hospital
 January 1 2023 Not Applicable
NCT05578443 Recruiting
Alzheimer''s Disease
Fujian Medical University Union Hospital
 October 1 2022 Phase 2
NCT05528744 Recruiting
Genetic Disease|Chopra-Amiel-Gordon Syndrome|CAGS|ANKRD17
Boston Children''s Hospital
 August 27 2022 --
NCT05533528 Recruiting
Periodontal Pocket|Periodontal Diseases|Periodontal Bone Loss
Universidad de Murcia
 May 3 2022 Not Applicable
NCT05118009 Completed
Myocardial Infarction Acute
National Defense Medical Center Taiwan
 May 1 2022 Not Applicable

Chemical Information & Solubility

Molecular Weight 490.72 Formula

C30H50O5
CAS No. 84605-18-5 SDF --
Smiles CC1(C(CCC23C1C(CC4C2(C3)CCC5(C4(CC(C5C6(CCC(O6)C(C)(C)O)C)O)C)C)O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 98 mg/mL ( (199.7 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 25 mg/mL

Water : Insoluble


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In vivo
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