Home Endocrinology & Hormones Glucocorticoid Receptor agonist Corticosterone For research use only.

Corticosterone

Synonyms: 17-deoxycortisol, 11β,21-dihydroxyprogesterone, NSC-9705

Corticosterone, the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.

Corticosterone Chemical Structure

Corticosterone Chemical Structure

CAS No. 50-22-6

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Corticosterone Related Products

Biological Activity

Description Corticosterone, the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.
Targets
Glucocorticoid receptor [2]
In vitro
In vitro Corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane. It enhances AMPAR-mediated miniature excitatory postsynaptic current (mEPSC) amplitude and surface expression of GluR1 and GluR2 subunits in hippocampal neurons, increases the surface mobility and synaptic content of AMPAR GluR2 subunits, and enhances L-type calcium currents in CA1 pyramidal neurons. Corticosterone operates through mineralocorticoid receptors and glucocorticoid receptors, both of which belong to the family of nuclear receptors that bind to response elements in the DNA, thus modifying the activity of responsive genes[1].
Cell Research Cell lines HEK293 cells
Concentrations 100 nM
Incubation Time 30 min
Method HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ∼50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.
Experimental Result Images Methods Biomarkers Images PMID
Growth inhibition assay Cell viability 30037364
In Vivo
In vivo Corticosterone plays an important role in regulating neuronal functions of the limbic system. After stress, the level of stress hormones such as corticosterone is markedly increased. Corticosterone exerts a time- and region-specific action on cellular physiology of limbic neurons[1].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05903716 Recruiting
Acne Vulgaris
Marmara University
 September 15 2022 --
NCT04930471 Unknown status
Autistic Disorder
Insel Gruppe AG University Hospital Bern|Lindenhofstiftung
 June 2021 --
NCT02826278 Unknown status
Newborn Genitalia
Hospices Civils de Lyon
 October 2014 --
NCT01666314 Completed
Prostate Cancer
Millennium Pharmaceuticals Inc.|Takeda
 August 20 2012 Phase 1|Phase 2
NCT01186484 Completed
Prostatic Neoplasms
Janssen Pharmaceutical K.K.
 June 1 2010 Phase 1

Chemical Information & Solubility

Molecular Weight 346.46 Formula

C21H30O4
CAS No. 50-22-6 SDF Download Corticosterone SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 69 mg/mL ( (199.15 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 69 mg/mL

Water : Insoluble


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