Corticosterone

Catalog No.S4752 Batch:S475202

Technical Data

Formula	C₂₁H₃₀O₄
Molecular Weight	346.46	CAS No.	50-22-6
Solubility (25°C)*	In vitro	DMSO	69 mg/mL (199.15 mM)
		Ethanol	9 mg/mL (25.97 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

Corticosterone, the major stress hormone, is an adrenocortical steroid that has modest but significant activities as a mineralocorticoid and a glucocorticoid.

Targets

Glucocorticoid receptor ^[2]

In vitro

Corticosterone, via SGK phosphorylation of GDI at Ser-213, increases the formation of GDI-Rab4 complex, facilitating the functional cycle of Rab4 and Rab4-mediated recycling of AMPARs to the synaptic membrane. It enhances AMPAR-mediated miniature excitatory postsynaptic current (mEPSC) amplitude and surface expression of GluR1 and GluR2 subunits in hippocampal neurons, increases the surface mobility and synaptic content of AMPAR GluR2 subunits, and enhances L-type calcium currents in CA1 pyramidal neurons. Corticosterone operates through mineralocorticoid receptors and glucocorticoid receptors, both of which belong to the family of nuclear receptors that bind to response elements in the DNA, thus modifying the activity of responsive genes^[1].

In vivo

Corticosterone plays an important role in regulating neuronal functions of the limbic system. After stress, the level of stress hormones such as corticosterone is markedly increased. Corticosterone exerts a time- and region-specific action on cellular physiology of limbic neurons^[1].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines HEK293 cells Concentrations 100 nM Incubation Time 30 min Method HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ∼50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.

Cell Assay:^{^[1]}

Cell lines
HEK293 cells
Concentrations
100 nM
Incubation Time
30 min
Method
HEK293 cells are grown in 6-cm dishes in 10% fetal bovine serum DMEM medium. When cells are 90% confluent, the medium is changed to 0.5% fetal bovine serum DMEM to limit serum-induced up-regulation of SGK. For in vitro phosphorylation analysis, the following approach is used. HEK293 cells are transfected with or without SGK1 small interfering RNA. One day after transfection, cells are treated without or with 100 nM corticosterone for 30 min, washed, and maintained in 0.5% fetal bovine serum DMEM for 1.5 h. Then cells are lysed in the CytoBuster protein extraction reagent containing protease inhibitors. Cell lysates are centrifuged at 16,000 × g at 4 °C for 20 min. The supernatants (40 μl, ∼50 μg of total protein) are incubated with 1 μg of purified GST fusion protein of wild-type GDI or its mutants for 30 min at 30 °C in the reaction buffer (30 mM HEPES, pH 7.5, 10 mM MgCl2, 30 μM ATP, 1 μCi of [γ-32P]ATP, 100 nM calyculin, 1 μM okadaic acid). SDS-PAGE is carried out, and phosphorylated GDI is visualized with autoradiography.

References

[1] Liu W, et al. J Biol Chem. 2010, 285(9):6101-8.

Selleck's Corticosterone has been cited by 7 publications

MicroRNA-451a is a candidate biomarker and therapeutic target for major depressive disorder [ Gen Psychiatr, 2024, 37(1):e101291]	PubMed: 38304710
Novel Estrogen Receptor Dimerization BRET-Based Biosensors for Screening Estrogenic Endocrine-Disrupting Chemicals [ Biomater Res, 2024, 28:0010]	PubMed: 38464469
Androgens improve ovarian follicle function impaired by glucocorticoids through an androgen-IGF1-FSH synergistic effect [ Front Endocrinol -Lausanne), 2022, 13:951928]	PubMed: 36339442
Vocal babbling in a wild parrot shows life history and endocrine affinities with human infants [ Proc Biol Sci, 2022, 289(1976):20220592]	PubMed: 35642373
miR-182 mediated the inhibitory effects of NF-κB on the GPR39/CREB/BDNF pathway in the hippocampus of mice with depressive-like behaviors [ Behav Brain Res, 2021, 418:113647]	PubMed: 34743948
Overexpression of SIRT1 Inhibits Corticosterone-Induced Autophagy [ Neuroscience, 2019, 411:11-22]	PubMed: 31146010
Impacts of ABCB1 (G1199A) polymorphism on resistance, uptake, and efflux to steroid drugs. [Peng R, et al. Xenobiotica, 2016, 46(10):948-52]	PubMed: 26822676

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