Home Transmembrane Transporters ATPase inhibitor Chlorpropamide For research use only.

Chlorpropamide

Chlorpropamide inhibits Na(+),K(+)-ATPase and stimulates a high affinity cyclic AMP-phosphodiesterase of isolated liver plasma membrane. Chlorpropamide is a sulfonylurea class drug for type 2 diabetes mellitus.

Chlorpropamide Chemical Structure

Chlorpropamide Chemical Structure

CAS No. 94-20-2

Purity & Quality Control

Batch: S416601 DMSO]55 mg/mL]false]Ethanol]55 mg/mL]false]Water]Insoluble]false Purity: 99.85%
99.85

Chlorpropamide Related Products

Signaling Pathway

ATPase Signaling Pathways

ATPase Signaling Pathway

Biological Activity

Description Chlorpropamide inhibits Na(+),K(+)-ATPase and stimulates a high affinity cyclic AMP-phosphodiesterase of isolated liver plasma membrane. Chlorpropamide is a sulfonylurea class drug for type 2 diabetes mellitus.
Targets
Na(+),K(+)-ATPase [4]
In vitro
In vitro

Chlorpropamide acts through a cyclic AMP-independent mechanism. The addition of 0.2 mM Chlorpropamide to hepatocytes isolated from fed rats, raises the cellular concentration of fructose-2,6-bisphosphate (F-2, 6-P2). The accumulation of F-2, 6-P2 caused by Chlorpropamide (1 mM) is parallel to the stimulation of L-lactate production (36.6 versus 26.1 μmol of lactate/g of cells) and to the inhibition of gluconeogenesis (0.57 versus 0.94 μmol of [U-14C]pyruvate converted to glucose/g of cells). Chlorpropamide enhances the inhibitory action evoked by insulin on glucagon-stimulated gluconeogenesis[1]. Chlorpropamide treatment has no effect on insulin binding, altering neither receptor number nor affinity in rat adipocytes. Chlorpropamide (175 μg/mL) enhances 2-deoxyglucose transport in both the absence (17%) and presence (20%) of insulin. Chlorpropamide significantly increases glucose metabolism and total lipids in both the absence (30%) and presence (31%) of insulin[2]. Chlorpropamide competitively inhibits antidiuretic hormone (ADH) binding and adenylyl cyclase (AC) stimulation with Ki of 2.8 mM and 250 μM, respectively, in the LLC-PK1 cell line. Chlorpropamide (333 μM) increases the apparent Ka of ADH for AC activation (0.31 vs. 0.08 nM) without affecting a maximal response. Twenty-four-hour Chlorpropamide exposure (100 μM) upregulates the ADH receptors without affecting affinity, which lowers Ka and increases basal AC activity and maximal response (1.86 vs. 1.35 and 14.9 vs. 10.6 fmol cAMP/min/1000 cells).

Chemical Information & Solubility

Molecular Weight 276.74 Formula

C10H13ClN2O3S
CAS No. 94-20-2 SDF Download Chlorpropamide SDF
Smiles CCCNC(=O)NS(=O)(=O)C1=CC=C(C=C1)Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 55 mg/mL ( (198.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 55 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Chlorpropamide | Chlorpropamide ic50 | Chlorpropamide price | Chlorpropamide cost | Chlorpropamide solubility dmso | Chlorpropamide purchase | Chlorpropamide manufacturer | Chlorpropamide research buy | Chlorpropamide order | Chlorpropamide mouse | Chlorpropamide chemical structure | Chlorpropamide mw | Chlorpropamide molecular weight | Chlorpropamide datasheet | Chlorpropamide supplier | Chlorpropamide in vitro | Chlorpropamide cell line | Chlorpropamide concentration | Chlorpropamide nmr