Home Epigenetics DNA Methyltransferase inhibitor Bobcat339 hydrochloride For research use only.

Bobcat339 hydrochloride

Synonyms: Bobcat339 HCl

Bobcat339 is a selective cytosine-based TET enzyme inhibitor with IC50 of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 can reduce DNA 5-hydroxymethylcytosine abundance by inhibiting TET enzyme function in living cells and provide support for its utility as a viable pharmacological probe.

Bobcat339 hydrochloride Chemical Structure

Bobcat339 hydrochloride Chemical Structure

CAS No. 2436747-44-1

Purity & Quality Control

Bobcat339 hydrochloride Related Products

Signaling Pathway

DNA Methyltransferase Signaling Pathways

DNA Methyltransferase Signaling Pathway

Biological Activity

Description Bobcat339 is a selective cytosine-based TET enzyme inhibitor with IC50 of 33 μM and 73 μM for TET1 and TET2, respectively. Bobcat339 can reduce DNA 5-hydroxymethylcytosine abundance by inhibiting TET enzyme function in living cells and provide support for its utility as a viable pharmacological probe.
Targets
TET1 [1]
(Cell-free assay)		 TET2 [1]
(Cell-free assay)
33 μM 73 μM
In vitro
In vitro

Bobcat339, the TET2 enzyme activity inhibitor, decreases the TET2 enzyme activity and protects cells from neurotoxin damage in SH-SY5Y cells.[2]
Cell Research Cell lines SH-SY5Y neuroblastoma cells
Concentrations 3 μM
Incubation Time 12 h
Method

The CCK-8 assay kit was used to determine cell viability. SH-SY5Y cells are seeded into 96-well plates at a density of 5,000 cells/well with 100 μl of cell medium in each well. Bobcat339 (3 μM) is preincubated with cells for 12 h before MPP+ (2.5 mM) treatment. Then, 10 μl of CCK-8 working solution is added to each well to incubate for about 1 h at 37 C. The wells containing only the culture medium served as blanks. The absorbance value at 450 nm is measured.
In Vivo
In vivo

Bobcat339 hydrochloride is a potent and selective cytosine-based inhibitor of TET enzyme.

Chemical Information & Solubility

Molecular Weight 334.2 Formula

C16H13Cl2N3O
CAS No. 2436747-44-1 SDF --
Smiles C1=CC=C(C=C1)C2=CC(=CC=C2)N3C=C(C(=NC3=O)N)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 60 mg/mL ( (179.53 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Bobcat339 hydrochloride | Bobcat339 hydrochloride supplier | purchase Bobcat339 hydrochloride | Bobcat339 hydrochloride cost | Bobcat339 hydrochloride manufacturer | order Bobcat339 hydrochloride | Bobcat339 hydrochloride distributor