Home Metabolism ATP-citrate lyase inhibitor BMS303141 For research use only.

BMS303141

BMS-303141 is a potent, cell-permeable inhibitor of ATP-citrate lyase (ACL) with IC50 of 0.13 μM. BMS-303141 shows inhibition of total lipid syntheses with IC50 of 8 μM in HepG2 cells.

BMS303141 Chemical Structure

BMS303141 Chemical Structure

CAS No. 943962-47-8

Purity & Quality Control

BMS303141 Related Products

Biological Activity

Description BMS-303141 is a potent, cell-permeable inhibitor of ATP-citrate lyase (ACL) with IC50 of 0.13 μM. BMS-303141 shows inhibition of total lipid syntheses with IC50 of 8 μM in HepG2 cells.
Targets
ACL [1]
(Cell-free assay)
0.13 μM
In vitro
In vitro

In HepG2 cells, BMS-303141 shows inhibition of total lipid syntheses with an IC50 of 8 μM, and no cytotoxicity up to 50 lM under a cell based Alamar Blue cytotoxicity assay.[2]
Cell Research Cell lines HepG2 cells
Concentrations --
Incubation Time 6 h
Method

Inhibition of total lipid synthesis assay. HepG2 cells are incubated for 6 h after addition of the testing compound and [14C]-alanine is added for the last 4 h of incubation. Cell lipids are extracted, separated by TLC. The incorporation of [14C]-labeled in the total lipids is measured.
In Vivo
In vivo

BMS-303141 lowers approximate 20-30% plasma cholesterol and triglycerides, as well as 30-50% fasting plasma glucose in high-fat fed mice.[2]
Animal Research Animal Models high-fat fed mice
Dosages 10 or 100 mg/kg
Administration o.g.

Chemical Information & Solubility

Molecular Weight 424.30 Formula

C19H15Cl2NO4S
CAS No. 943962-47-8 SDF --
Smiles COC1=C(C=C(C=C1)C2=CC=CC=C2)NS(=O)(=O)C3=C(C(=CC(=C3)Cl)Cl)O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 85 mg/mL ( (200.32 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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