Home Angiogenesis Bcr-Abl inhibitor - NF-κB inhibitor - CaMK modulator Berbamine dihydrochloride For research use only.

Berbamine dihydrochloride

Synonyms: BA, BBM

Berbamine (BA, BBM) dihydrochloride, a traditional Chinese medicines extracted from Berberis amurensis (xiaoboan), is a novel inhibitor of bcr/abl fusion gene with potent anti-leukemia activity and also an inhibitor of NF-κB. Berbamine (BA, BBM) dihydrochloride induces apoptosis in human myeloma cells and inhibits the growth of cancer cells by targeting Ca²⁺/calmodulin-dependent protein kinase II (CaMKII).

Berbamine dihydrochloride Chemical Structure

Berbamine dihydrochloride Chemical Structure

CAS No. 6078-17-7

Purity & Quality Control

Berbamine dihydrochloride Related Products

Signaling Pathway

Bcr-Abl Signaling Pathways

Bcr-Abl Signaling Pathway

Biological Activity

Description Berbamine (BA, BBM) dihydrochloride, a traditional Chinese medicines extracted from Berberis amurensis (xiaoboan), is a novel inhibitor of bcr/abl fusion gene with potent anti-leukemia activity and also an inhibitor of NF-κB. Berbamine (BA, BBM) dihydrochloride induces apoptosis in human myeloma cells and inhibits the growth of cancer cells by targeting Ca²⁺/calmodulin-dependent protein kinase II (CaMKII).
Targets
Bcr-Abl [4] NF-κB [5] CaMKII [1]
In vitro
In vitro

Berbamine (BBM) potently suppresses liver cancer cell proliferation and induces cancer cell death by targeting Ca2+/calmodulin-dependent protein kinase II (CAMKII). Through targeting CAMKII, BBM's inhibition on cancer cells might have broader effects beyond affecting JAK/STAT3 and p210bcr-abl, because CAMKIIγ directly impacts many other signal pathways including STAT1, NF-κB, JNK, ERK1/2, FOXO1, and Wnt/β-catenin[1]. BBM can effectively inhibit tumor metastasis by suppressing cell proliferation, migration and invasion in highly metastatic breast cancer cells under in vitro condition[2]. Berbamine inhibits proliferation of K562-r cells both in vitro and in vivo. Berbamine-induced apoptosis in K562-r cells appear to occur through a mechanism involving Bcl-2 family proteins, as well as mdr-1 mRNA and P-gp protein. Berbamine in combination restore the chemo-sensitivity of K562-r cells[3].
Cell Research Cell lines epithelial liver cancer cell lines, including Huh7, HepG2, MHCC97H, and PLC/PRF/5
Concentrations 0-80 μg/ml
Incubation Time 72 h
Method

Cell proliferation is assayed 72 hours after BBM treatment for liver cancer cells with epithelial morphology.
In Vivo
In vivo

BBM inhibits the in vivo tumorigenicity of liver cancer cells in NOD/SCID mice, and down-regulated the self-renewal abilities of liver cancer initiating cells[1]. BBM shows its anticancer activity by induction of apoptosis, cell cycle arrest and reversing multidrug resistance. Though BBM is a potent drug but its half-life in blood plasma is very short, owing to its quick renal clearance[2].
Animal Research Animal Models NOD/SCID mouse
Dosages 100 mg/kg
Administration oral

Chemical Information & Solubility

Molecular Weight 681.65 Formula

C37H40N2O6.2HCl
CAS No. 6078-17-7 SDF Download Berbamine dihydrochloride SDF
Smiles CN1CCC2=CC(=C3C=C2C1CC4=CC=C(C=C4)OC5=C(C=CC(=C5)CC6C7=C(O3)C(=C(C=C7CCN6C)OC)OC)O)OC.Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (146.7 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 50 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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