Home Apoptosis TRADD inhibitor Apostatin-1 (Apt-1) For research use only.

Apostatin-1 (Apt-1)

Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.

Apostatin-1 (Apt-1) Chemical Structure

Apostatin-1 (Apt-1) Chemical Structure

CAS No. 2559703-06-7

Purity & Quality Control

Batch: S970501 DMSO]69 mg/mL]false]Ethanol]9 mg/mL]false]Water]Insoluble]false Purity: 99.56%
99.56

Apostatin-1 (Apt-1) Related Products

Biological Activity

Description Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.
Targets
TRADD [1]
In vitro
In vitro

Apostatin-1 (Apt-1) is a TRADD inhibitor by combining with the pocket on the N-terminal TRAF2 binding domain of TRADD (TRADD-N) to modulate the ubiquitination of RIPK1 and beclin 1. Apostatin-1 effectively inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) in vitro.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research Cell lines Jurkat (ATCC TIB-152), MEF (CF-1) (ATCC SCRC-1040)
Concentrations 10 µM
Incubation Time 24 h for Jurkat cells; 8 h for mouse embryonic fibroblasts (MEFs)
Method

Jurkat cells were treated with Velcade (50 nM) and  Apt-1 (10 μM) for 24 h and cell viability was measured; In MEFs were treated with mTNF (1 ng/mL) and 5Z-7- Oxozeaenol (0.5 μM) to achieve RIPK1-dependent apoptosis (RDA),  added Apt-1 at different concentrations for 8 h and cell survival was measured.

Chemical Information & Solubility

Molecular Weight 345.50 Formula

C19H27N3OS
CAS No. 2559703-06-7 SDF --
Smiles CN1CCN=C1SC(C(=O)NC2CCCCCC2)C3=CC=CC=C3
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 69 mg/mL ( (199.71 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 9 mg/mL

Water : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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