Apostatin-1 (Apt-1)

Catalog No.S9705 Batch:S970501

Technical Data

Formula	C₁₉H₂₇N₃OS
Molecular Weight	345.50	CAS No.	2559703-06-7
Solubility (25°C)*	In vitro	DMSO	69 mg/mL (199.71 mM)
		Ethanol	9 mg/mL (26.04 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Apostatin-1 (Apt-1) is a novel TRADD inhibitor. Apostatin-1 binds to a pocket on the N-terminal TRAF2-binding domain of TRADD (TRADD-N). Apostatin-1 inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) with an IC50 of about 1 μM.

Targets

TRADD ^[1]

In vitro

Apostatin-1 (Apt-1) is a TRADD inhibitor by combining with the pocket on the N-terminal TRAF2 binding domain of TRADD (TRADD-N) to modulate the ubiquitination of RIPK1 and beclin 1. Apostatin-1 effectively inhibits bortezomib-induced apoptosis and RIPK1-dependent apoptosis (RDA) in vitro.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Assay:^{<sup><a class="sref" href="#s_ref">[1]</a></sup>}	Cell lines Jurkat (ATCC TIB-152), MEF (CF-1) (ATCC SCRC-1040) Concentrations 10 µM Incubation Time 24 h for Jurkat cells; 8 h for mouse embryonic fibroblasts (MEFs) Method Jurkat cells were treated with Velcade (50 nM) and Apt-1 (10 μM) for 24 h and cell viability was measured; In MEFs were treated with mTNF (1 ng/mL) and 5Z-7- Oxozeaenol (0.5 μM) to achieve RIPK1-dependent apoptosis (RDA), added Apt-1 at different concentrations for 8 h and cell survival was measured.

Cell Assay:^{<sup><a class="sref" href="#s_ref">[1]</a></sup>}

Cell lines
Jurkat (ATCC TIB-152), MEF (CF-1) (ATCC SCRC-1040)
Concentrations
10 µM
Incubation Time
24 h for Jurkat cells; 8 h for mouse embryonic fibroblasts (MEFs)
Method
Jurkat cells were treated with Velcade (50 nM) and Apt-1 (10 μM) for 24 h and cell viability was measured; In MEFs were treated with mTNF (1 ng/mL) and 5Z-7- Oxozeaenol (0.5 μM) to achieve RIPK1-dependent apoptosis (RDA), added Apt-1 at different concentrations for 8 h and cell survival was measured.

References

[1] Daichao Xu, et al. Nature. 2020 Nov;587(7832):133-138.

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.