Home Metabolism Aldose Reductase inhibitor Isoliquiritigenin For research use only.

Isoliquiritigenin

Isoliquiritigenin, an anti-tumor flavonoid from the root of Glycyrrhiza glabra, inhibits aldose reductase with an IC50 of 320 nM.

Isoliquiritigenin Chemical Structure

Isoliquiritigenin Chemical Structure

CAS No. 961-29-5

Purity & Quality Control

Isoliquiritigenin Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
KB cells Growth inhibition assay Compound concentration required to reduce the exponential growth of KB cells by 50%, CC50=12 μM 9767632
MT-4 cells Growth inhibition assay Compound concentration required to reduce the exponential growth of MT-4 cells by 50%, CC50=7.4 μM 9767632
Hepa lclc7 cells Cytotoxicity assay Cytotoxicity against mouse Hepa lclc7 cells, IC50=39 μM 18076142
J774.1 cells Function assay 24 h Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=29.3 μM 15974608
colon 26-L5 cells Cytotoxicity assay 72 h Cytotoxicity against mouse colon 26-L5 cells after 72 hrs by MTT assay, IC50=21.8 μM 18440233
MCF7 cells Cytotoxicity assay 25-150 μM 72 h Cytotoxicity against human MCF7 cells at 25 to 150 uM after 72 hrs by MTT assay 18603336
CHO cells Cytotoxicity assay Cytotoxicity against CHO cells by MTT assay, IC50=45.9 μM 19572738
human T47D cells Function assay 96 h Estrogenic activity in human T47D cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay 19928832
MCF7 cells Function assay 96 h Estrogenic activity in human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol after 96 hrs by alamar blue assay 19928832
MCF7 cells Function assay Estrogenic activity in luciferase transfected human MCF7 cells assessed as drug level causing stimulation of cell proliferation equivalent to 10 pM estradiol by luciferase reporter gene assay 19928832
mouse NIH3T3 cells Function assay 8 h Inhibition of cobalt chloride-induced HIF-1 activation expressed in mouse NIH3T3 cells after 8 hrs by luciferase reporter gene assay, IC50=4.8 μM 21112783
OE21 cells Cytotoxicity assay 72 h Cytotoxicity against human OE21 cells after 72 hrs by MTT assay, IC50=48 μM 21696954
U937 cells Function assay 5 ug/ml 24 h Antiinflammatory activity in human U937 cells assessed as inhibition of LPS-induced CCL5 secretion at 5 ug/ml after 24 hrs by ELISA relative to control 21866899
dog MDCK cells Function assay 20 ug/mL 1 h Antiviral activity against Influenza virus A/Puerto Rico/8/34 H1N1 infected in dog MDCK cells assessed as inhibition of viral replication at 20 ug/mL incubated at 1 hr post-infection measured after 24 hrs by hemagglutininating unit assay relative to control 22743086
dog MDCK cells Function assay 8 h Inhibition of PKC/p38MAPK-mediated nuclear-cytoplasmic viral ribonucleoprotein export in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 8 hrs post-infection by inmmunofluorescence 22743086
NCI-H292 cells Function assay Inhibition of PKC-mediated PKD phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis 22743086
NCI-H292 cells Function assay Inhibition of PKC-mediated p38MAPK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis 22743086
NCI-H292 cells Function assay Inhibition of PKC-mediated JNK phosphorylation in human NCI-H292 cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 6 hrs post-infection by immunoblotting analysis 22743086
dog MDCK cells Function assay Restoration of GSH level in dog MDCK cells infected with Influenza virus A/Puerto Rico/8/34 H1N1 measured at 24 hrs post-infection by DTNB-based colometric assay 22743086
RBL-1 cells Function assay In vitro inhibition against 5-lipoxygenase in RBL-1 cells was determined, IC50=35 μM 8254620
PC3 cells Cytotoxicity assay Cytotoxicity against human PC3 cells, IC50=46.4 μM 16441066
HUVEC Cytotoxicity assay 48 h Toxicity against HUVEC incubated for 48 hrs by MTT assay, IC50=41.17 μM 25590864
K562 cells Cytotoxicity assay 48 h Antitumor activity against human K562 cells incubated for 48 hrs by MTT assay, IC50=29.27 μM 25590864
Hepa-1c1c7 cells Function assay Induction of quinone reductase activity in mouse Hepa-1c1c7 cells assessed as drug level required to double enzyme activity 12762787
Hepa lclc7 cells Function assay Induction of mouse quinone reductase in mouse Hepa lclc7 cells assessed as concentration required to double enzyme activity 18076142
U937 cells Function assay 5 ug/ml Inhibition of LPS-induced AP-1 activation in human U937 cells at 5 ug/ml after 1 hr relative to control 21866899
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Biological Activity

Description Isoliquiritigenin, an anti-tumor flavonoid from the root of Glycyrrhiza glabra, inhibits aldose reductase with an IC50 of 320 nM.
Targets
Aldose reductase [1]
(Cell-free assay)
320 nM
In vitro
In vitro Isoliquiritigenin may be effective in preventing diabetic complications through inhibiting rat lens aldose reductase with an IC50 of 320 nM and sorbitol accumulation in human red blood cells with an IC50 of 2.0 μM. [1] Isoliquiritigenin may also serve as a skin-lightening agent by inhibiting mono- and diphenolase tyrosinase activities with an IC50 of 8.1 μM. [2] Isoliquiritigenin induces cell cycle arrest and cell growth inhibition in DU145 and LNCaP prostate cancer cell lines. [3] Isoliquiritigenin induces apoptosis of human gastric cancer MGC-803 cells through increasing the intracellular free calcium concentration and decreasing the mitochondrial transmembrane potential (Deltapsi(m)). [4]
In Vivo
In vivo LD50: Mice >6g/kg (i.g.) [5]

Chemical Information & Solubility

Molecular Weight 256.25 Formula

C15H12O4
CAS No. 961-29-5 SDF Download Isoliquiritigenin SDF
Smiles C1=CC(=CC=C1C=CC(=O)C2=C(C=C(C=C2)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

Ethanol : 26 mg/mL

DMSO : 12 mg/mL ( (46.82 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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