Home PI3K/Akt/mTOR GSK-3 inhibitor Indirubin (NSC 105327) For research use only.

Indirubin (NSC 105327)

Indirubin (NSC 105327) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.

Indirubin (NSC 105327) Chemical Structure

Indirubin (NSC 105327) Chemical Structure

CAS No. 479-41-4

Purity & Quality Control

Indirubin (NSC 105327) Related Products

Signaling Pathway

GSK-3 Signaling Pathways

GSK-3 Signaling Pathway

Biological Activity

Description Indirubin (NSC 105327) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.
Targets
GSK-3β [2] CDK2/CyclinA [1] CDK5/p35 [1] CDK1/CyclinB [1] CDK4/CyclinD1 [1] Click to View More Targets
0.6 μM 2.2 μM 5.5 μM 10 μM 12 μM
In vitro
In vitro Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. [1] Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. [2] It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65. [3]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02088281 Completed
Psoriasis Vulgaris
Chang Gung Memorial Hospital|National Science Council Taiwan
 November 2012 Phase 2

Chemical Information & Solubility

Molecular Weight 262.26 Formula

C16H10N2O2
CAS No. 479-41-4 SDF Download Indirubin (NSC 105327) SDF
Smiles C1=CC=C2C(=C1)C(=C(N2)O)C3=NC4=CC=CC=C4C3=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 21.875 mg/mL ( (83.4 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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