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EIPA

Name: EIPA
Brand: Selleck Chemicals
SKU: S9849
Rating: 5 (4 reviews)

Synonyms: L593754; MH 12-43

Catalog No.S9849 Purity:99.77%

EIPA (L593754; MH 12-43) acts as an inhibitor of macropinocytosis and sodium-hydrogen exchangers(NHE) (IC50=0.033μg/mL). EIPA blocks the activity of Na(+)/H(+) exchanger, which are plasma membrane proteins implicated in all forms of macropinocytosis.

EIPA Chemical Structure

CAS No. 1154-25-2

Purity & Quality Control

Batch: Purity: 99.77%

99.77

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Signaling Pathway

Sodium Channel Signaling Pathway

Biological Activity

Description

Targets

NHE ^[1]
(in the Sodium-hydrogen exchanger activity assay)

0.033 μg/mL

In vitro
In vitro	EIPA rapidly and reversibly block Ca²⁺-activated TRPP3 channel activation, with IC₅₀s of 10.5 μM.^[2]
Cell Research	Cell lines	Xenopus laevis oocyte
	Concentrations	100 μM
	Incubation Time	30 min
	Method	The uptake of radioactive ₄₅Ca₂₊ mediated by TRPP3 channel is tested using the standard NaCl-containing solution, plus 1 mM nonradiolabeled CaCl2 and radiolabeled ₄₅Ca₂₊ in the presence and absence of 100 μM EIPA. Control uptake level is obtained using H2O-injected oocytes.

In Vivo
In vivo	EIPA downregulates endothelial cell activation of NF-kappaB and VCAM-1 expression and attenuates the early inflammatory stages of atherogenesis and stent intimal formation^[3].
Animal Research	Animal Models	Murine Model of Atherogenesis (apoE-/- mice)
	Dosages	3 mg/kg
	Administration	s.c.

References

Chemical Information & Solubility

Molecular Weight	299.76	Formula	C₁₁H₁₈ClN₇O
CAS No.	1154-25-2	SDF	--
Smiles	CCN(C(C)C)C1=C(Cl)N=C(C(=N1)N)C(=O)NC(N)=N
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 60 mg/mL ( (200.16 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 30 mg/mL

Water : 2 mg/mL

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	5% DMSO 1 95% Corn oil	5.5mg/ml (18.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	5.5mg/ml (18.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	2.0mg/ml (6.67mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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