EIPA

Catalog No.S9849 Batch:S984901

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Technical Data

Formula

C11H18ClN7O
Molecular Weight 299.76 CAS No. 1154-25-2
Solubility (25°C)* In vitro DMSO 60 mg/mL (200.16 mM)
Ethanol 30 mg/mL (100.08 mM)
Water 2 mg/mL (6.67 mM)
In vivo (Add solvents to the product individually and in order)
Clear solution
5%DMSO 40%PEG300 5%Tween80 50%ddH2O
5.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results. 
Clear solution
5% DMSO 95% Corn oil
5.5mg/ml Taking the 1 mL working solution as an example, add 50 μL of 110 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results. 
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description EIPA (L593754; MH 12-43) acts as an inhibitor of macropinocytosis and sodium-hydrogen exchangers(NHE) (IC50=0.033μg/mL). EIPA blocks the activity of Na(+)/H(+) exchanger, which are plasma membrane proteins implicated in all forms of macropinocytosis.
Targets
NHE [1]
(in the Sodium-hydrogen exchanger activity assay)
0.033 μg/mL
In vitro

EIPA rapidly and reversibly block Ca2+-activated TRPP3 channel activation, with IC50s of 10.5 μM.[2]
In vivo

EIPA downregulates endothelial cell activation of NF-kappaB and VCAM-1 expression and attenuates the early inflammatory stages of atherogenesis and stent intimal formation[3].

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Xenopus laevis oocyte

  • Concentrations

    100 μM

  • Incubation Time

    30 min

  • Method

    The uptake of radioactive 45Ca2+ mediated by TRPP3 channel  is tested using the standard NaCl-containing solution, plus 1 mM nonradiolabeled CaCl2 and radiolabeled 45Ca2+ in the presence and absence of 100 μM EIPA. Control uptake level is obtained using H2O-injected oocytes.
Animal Study:

[3]
  • Animal Models

    Murine Model of Atherogenesis (apoE-/- mice)

  • Dosages

    3 mg/kg

  • Administration

    s.c.

Selleck's EIPA has been cited by 4 publications

WNT Oncogenic Transcription Requires MYC Suppression of Lysosomal Activity and EPCAM Stabilization in Gastric Tumors [ Gastroenterology, 2024, S0016-5085(24)05182-5] PubMed: 38971196
Porcine deltacoronavirus enters ST cells by clathrin-mediated endocytosis and does not require Rab5, Rab7, or Rab11 [ Microbiol Spectr, 2023, 10.1128/spectrum.02553-23] PubMed: 37962380
In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish [ Int J Mol Sci, 2022, 23(8)4419] PubMed: 35457237
Porcine Deltacoronavirus (PDCoV) Entry into PK-15 Cells by Caveolae-Mediated Endocytosis [ Viruses, 2022, 14(3)496] PubMed: 35336903

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.