Home Cytoskeletal Signaling MLCK inhibitor 2,3-Butanedione-2-monoxime For research use only.

2,3-Butanedione-2-monoxime

Synonyms: Diacetyl monoxime, Diacetylmonoxime

2,3-Butanedione monoxime (BDM, Diacetyl monoxime, Diacetylmonoxime) is the well-characterized, low-affinity, non-competitive inhibitor of skeletal muscle myosin-II and inhibits skeletal and cardiac muscle contraction.

2,3-Butanedione-2-monoxime Chemical Structure

2,3-Butanedione-2-monoxime Chemical Structure

CAS No. 57-71-6

Purity & Quality Control

Batch: S369901 DMSO]20 mg/mL]false]Water]20 mg/mL]false]Ethanol]20 mg/mL]false Purity: 99.16%
99.16

2,3-Butanedione-2-monoxime Related Products

Biological Activity

Description 2,3-Butanedione monoxime (BDM, Diacetyl monoxime, Diacetylmonoxime) is the well-characterized, low-affinity, non-competitive inhibitor of skeletal muscle myosin-II and inhibits skeletal and cardiac muscle contraction.
Targets
skeletal muscle myosin-II [1]
In vitro
In vitro BDM inhibits skeletal muscle myosin-II, but it does not inhibit the ATPase activity of the other myosins. BDM has a broad effect on many non-myosin proteins (many uncharacterized), and thus should not be used in whole-cell experiments as a myosin inhibitor[1]. BDM inhibits the L-type Ca2+-channel and reduces the opening probability, thereby increasing the closed time of this particular channel. BDM drives nonspecific protein dephosphorylation of many cellular proteins, including ion channels[2].
In Vivo
In vivo BDM significant lowers the blood pressure in normo- and hypertensive rats. BDM has been shown to improve right atrial function in a porcine model of allogenic heart transplantation[2].
Animal Research Animal Models Male SD rats
Dosages 250 mg/kg
Administration i.p.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05391776 Unknown status
Osteoporosis
HJB (Hangzhou) Co. Ltd.
 April 28 2022 Phase 1
NCT01721187 Completed
Fasting|Fed
Duke-NUS Graduate Medical School|Singapore Institute for Clinical Sciences
 October 2012 --

Chemical Information & Solubility

Molecular Weight 101.10 Formula

C4H7NO2
CAS No. 57-71-6 SDF Download 2,3-Butanedione-2-monoxime SDF
Smiles CC(=NO)C(=O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 20 mg/mL ( (197.82 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 20 mg/mL

Ethanol : 20 mg/mL


Molecular Weight Calculator

In vivo
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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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