Home GPCR & G Protein Adenosine Receptor antagonist ZM241385 For research use only.

ZM241385

ZM-241385 is a high affinity antagonist ligand selective for the adenosine A2A receptor.

ZM241385 Chemical Structure

ZM241385 Chemical Structure

CAS No. 139180-30-6

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ZM241385 Related Products

Biological Activity

Description ZM-241385 is a high affinity antagonist ligand selective for the adenosine A2A receptor.
Targets
adenosine A2A receptor [3]
In vitro
In vitro ZM 241385 has high affinity for A2a receptors. In rat phaeochromocytoma cell membranes, ZM 241385 displaces binding of tritiated 5'-N-ethylcarboxamidoadenosine (NECA) with a pIC50 of 9.52[3]. ZM241385 delays the appearance of anoxic depolarization (AD), a phenomenon strictly related to cell damage and death, protect from the synaptic activity depression brought about by a severe (7 min) OGD period, and protect CA1 neuron and astrocyte from injury[4].
Cell Research Cell lines PC12 cells
Concentrations 1 μM
Incubation Time 24 h
Method

Nitric oxide (NO) production is assessed by measuring the amount of nitrite, a stable metabolic product of NO that provides an indirect measurement of NO, by using the Griess diazotization reaction. Briefly, 24 h after incubation with 1.0 µM CG21680 or 1.0 µM ZM241385 or exposure to SMF, samples of medium (150 µl) are collected from cells and mixed with 130 µl dH2O and with 20 µl Griess reagent using instruction supplied by the manufacturer. After a 30 min incubation period at room temperature, the samples are evaluated spectrophotometrically at 548 nm and OD values-in comparison with a standard curve is determined in culture medium by using serial dilutions of sodium nitrite-represented total stable metabolites of NO.
In Vivo
In vivo ZM-241385 significantly blunts the hypotensive effects of CCPA and NECA without altering the bradycardia induced by these agonists. It blocks the infarct size-reducing effects(cardioprotective effects) of this two distinct adenosine receptor agonists[1].
Animal Research Animal Models Adult Sprague-Dawley rats 
Dosages 1.5 mg/kg
Administration i.v.

Chemical Information & Solubility

Molecular Weight 337.34 Formula

C16H15N7O2
CAS No. 139180-30-6 SDF Download ZM241385 SDF
Smiles C1=COC(=C1)C2=NN3C(=NC(=NC3=N2)NCCC4=CC=C(C=C4)O)N
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 67 mg/mL ( (198.61 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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