Home Proteases Cysteine Protease inhibitor Z-FA-FMK For research use only.

Z-FA-FMK

Synonyms: (1S)-Z-FA-FMK

Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.

Z-FA-FMK Chemical Structure

Z-FA-FMK Chemical Structure

CAS No. 197855-65-5

Purity & Quality Control

Z-FA-FMK Related Products

Biological Activity

Description Z-FA-FMK ((1S)-Z-FA-FMK) is an irreversible cysteine protease inhibitor, and also inhibits effector caspases.
Targets
cysteine protease [1]
In vitro
In vitro Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. [2] Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. [3] Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro. [4]
Cell Research Cell lines PBMCs
Concentrations ~100 μM
Incubation Time 72 hours
Method T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester.
In Vivo
In vivo Z-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. [4] Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice. [5]

Chemical Information & Solubility

Molecular Weight 386.42 Formula

C21 H23 F N2 O4
CAS No. 197855-65-5 SDF Download Z-FA-FMK SDF
Smiles CC(C(=O)CF)NC(=O)C(CC1=CC=CC=C1)NC(=O)OCC2=CC=CC=C2
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 77 mg/mL ( (199.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 48 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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