Home PI3K/Akt/mTOR PTEN inhibitor VO-Ohpic trihydrate For research use only.

VO-Ohpic trihydrate

VO-Ohpic is a potent inhibitor of PTEN (phosphatase and tensin homolog) with IC50 of 35 nM.

VO-Ohpic trihydrate Chemical Structure

VO-Ohpic trihydrate Chemical Structure

CAS No. 476310-60-8

Purity & Quality Control

VO-Ohpic trihydrate Related Products

Biological Activity

Description VO-Ohpic is a potent inhibitor of PTEN (phosphatase and tensin homolog) with IC50 of 35 nM.
Targets
PTEN [1]
(Cell-free assay)
35 nM
In vitro
In vitro VO-OHpic is a potent small-molecule compound that specifically inhibits PTEN’s cellular enzymatic activity, which in turn activates downstream targets such as Akt and FoxO3a. Glucose uptake into adipocytes is dramatically enhanced upon PTEN inhibition with VO-OHpic. PTEN inhibitor accelerates wound healing in fibroblasts[1]. VO-OHpic inhibits cell viability, cell proliferation and colony formation, and induces senescence-associated β-galactosidase activity in Hep3B (low PTEN expression) and to a lesser extent in PLC/PRF/5 (high PTEN expression) cells, but not in PTEN-negative SNU475 cells[2].
Cell Research Cell lines The human hepatocellular carcinoma cell lines Hep3B, PLC/PRF/5, and SNU475
Concentrations 0-5 μM
Incubation Time 72 h
Method Cell proliferation is determined by estimating the amount of bromodeoxyuridine (BrdU) incorporation into DNA by a colorimetric immunoassay. 3×103 cells are cultured in 96-well plates with varying concentrations of VO-OHpic for 72 hours. BrdU is added 24 hours before the end of the treatments. Results are expressed as the percentage inhibition of BrdU incorporation over the control.
In Vivo
In vivo VO-Ohpic significantly inhibits tumor growth in nude mice bearing xenografts of Hep3B cells[2]. VO-Ohpic administered to C57BL6 mice 30 minutes prior to Kcl-induced asystolic cardiac arrest significantly increases survival, LVPmax and dP/dt max with continued benefit. VO-OHpic also significantly increases lactate clearance and decreases plasma glucose level[3].
Animal Research Animal Models Male nude athymic mice
Dosages 10 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 415.20 Formula

C12H9N2O8V.3H2O.H
CAS No. 476310-60-8 SDF Download VO-Ohpic trihydrate SDF
Smiles [H+].C1=CC(=C(N=C1)C(=O)[O-])O.C1=CC(=C(N=C1)C(=O)[O-])[O-].O.O[V]=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 72 mg/mL ( (173.41 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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