Home Microbiology Ribosomal Peptidyl Transferase inhibitor Tiamulin For research use only.

Tiamulin

Tiamulin is a semisynthetic pleuromutilin antibiotic that binds to the ribosomal peptidyl transferase centre and inhibits protein synthesis. Tiamulin has sustained antibacterial activity against specified bacterial species and rapidly growing and fastidious animal pathogens.

Tiamulin Chemical Structure

Tiamulin Chemical Structure

CAS No. 55297-95-5

Purity & Quality Control

Batch: S504001 DMSO]98 mg/mL]false]Water]98 mg/mL]false]Ethanol]98 mg/mL]false Purity: 98%
98

Tiamulin Related Products

Biological Activity

Description Tiamulin is a semisynthetic pleuromutilin antibiotic that binds to the ribosomal peptidyl transferase centre and inhibits protein synthesis. Tiamulin has sustained antibacterial activity against specified bacterial species and rapidly growing and fastidious animal pathogens.
In vitro
In vitro Tiamulin has a remarkable effect on decreasing the activity of CD73 in vitro and dramatically inhibits colony formation of MDA-MB-231 and 4 T1 cells[1]. Tiamulin is located within the peptidyl transferase center (PTC) of the 50S ribosomal subunit with its tricyclic mutilin core positioned in a tight pocket at the A-tRNA binding site. Tiamulin directly inhibits peptide bond formation. It interacts with 23S rRNA and clearly interferes with the correct positioning of both A- and P-site substrates[2].
Cell Research Cell lines MDA-MB-231 or 4 T1 cells
Concentrations 0, 6.25, 12.5, 25, 50 or 100 μg/ml
Incubation Time 0, 12, 24, 36 or 48 h
Method

MDA-MB-231 or 4 T1 cells are seeded in 96-well plates at 2 × 104 cells per well and incubated overnight before attached. Then, cells are treated without (control group) or with different concentrations of THF (0, 6.25, 12.5, 25, 50 or 100 μg/ml). After 0, 12, 24, 36 or 48 h, cell viability is assessed by using CCK-8 assays.
In Vivo
In vivo Tiamulin inhibits breast tumor growth and metastasis, mainly achieved by affecting the activity but not expression of CD73. The antitumor effect of Tiamulin results in inhibition of metastasis and angiogenesis[1].
Animal Research Animal Models Four-week old female BABL/c mice injected subcutaneously with 4 T1 cells
Dosages 80 mg/kg and 160 mg/kg
Administration by oral gavage

Chemical Information & Solubility

Molecular Weight 493.74 Formula

C28H47NO4S
CAS No. 55297-95-5 SDF --
Smiles CCN(CC)CCSCC(=O)OC1CC(C(C(C23CCC(C1(C2C(=O)CC3)C)C)C)O)(C)C=C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 98 mg/mL ( (198.48 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 98 mg/mL

Ethanol : 98 mg/mL


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In vivo
Batch:

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In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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