Home Protein Tyrosine Kinase IGF-1R inhibitor - Other inhibitor SBI-477 For research use only.

SBI-477

SBI-477 is an insulin signaling inhibitor that deactivates the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain-containing 4 (ARRDC4). SBI-477 inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes.

SBI-477 Chemical Structure

SBI-477 Chemical Structure

CAS No. 781628-99-7

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SBI-477 Related Products

Signaling Pathway

IGF-1R Signaling Pathways

IGF-1R Signaling Pathway

Biological Activity

Description SBI-477 is an insulin signaling inhibitor that deactivates the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain-containing 4 (ARRDC4). SBI-477 inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes.
Targets
MondoA [1] TXNIP [1] ARRDC4 [1] TAG [1]
In vitro
In vitro

SBI-477, that coordinately inhibits triacylglyceride (TAG) synthesis and enhances basal glucose uptake in human skeletal myocytes, stimulates insulin signaling by deactivating the transcription factor MondoA, leading to reduced expression of the insulin pathway suppressors thioredoxin-interacting protein (TXNIP) and arrestin domain containing 4 (ARRDC4).[1]
Cell Research Cell lines Primary human skeletal myotubes
Concentrations --
Incubation Time 24 h
Method

Primary human skeletal myotubes were grown and differentiated in 24-well plates. Cells were   treated with the indicated concentration of SBI-477 for 24 h. Following compound treatment, cells were rinsed three times with PBS and then incubated in 125μM [<sup>3</sup>H]-palmitic acid (60 Ci/mmol) bound to fatty acid free albumin containing 1mM carnitine for 2 hours at 37℃. The cell medium was transferred to a tube containing cold 10% trichloroacetic acid (TCA). The tubes were centrifuged at 8,500 x g for 10 minutes at 4℃. The supernatant was immediately removed, mixed with 6N NaOH, and applied to ion-exchange resin. The eluate was collected, measured by liquid scintillation analyzer and normalized to total protein amount. The amount of cell protein was measured.

Chemical Information & Solubility

Molecular Weight 483.54 Formula

C24H25N3O6S
CAS No. 781628-99-7 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 97 mg/mL ( (200.6 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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