Home Immunology & Inflammation NADPH-oxidase inhibitor - ROS activator - Apoptosis related activator - Mitophagy activator - Mitochondrial Metabolism modulator Rotenone For research use only.

Rotenone

Synonyms: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

Rotenone is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.

Rotenone Chemical Structure

Rotenone Chemical Structure

CAS No. 83-79-4

Purity & Quality Control

Rotenone Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 Function assay Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25C interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
HEK293 Function assay Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25A interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
HEK293 Function assay Effect on p53 expressed in HEK293 cells assessed as effect on p53-p53 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
HEK293 Function assay Inhibition of of c-Jun expressed in HEK293 cells assessed as induction of protein interaction in presence of camptothecin with Pin1 by EYFP based reporter gene assay 16680159
HEK293 Function assay Effect on cofilin1 expressed in HEK293 cells assessed as effect on cofilin1; Limk2 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay 16680159
MG63 Function assay 1 uM 1 hr Increase in lactate production in MG63 cells at 1 uM after 1 hr by ELISA 17088489
U937 Antiproliferative assay 72 hrs Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay, IC50=0.07μM 17158054
U937 Antiproliferative assay 48 hrs Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay, IC50=0.2μM 17158054
U937 Antiproliferative assay 24 hrs Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay, IC50=13μM 17158054
HT1080 Function assay Inhibition of proMMP2 production in human HT1080 cells by gelatin zymography analysis 17158054
HT1080 Function assay Inhibition of proMMP9 production in human HT1080 cells by gelatin zymography analysis 17158054
neural precursor cells Function assay Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay 17417631
HepG2 Antitumor assay 24 hrs Antitumor activity against human HepG2 cells after 24 hrs by MTT assay, ED50=15μM 17482824
A549 Antitumor assay 24 hrs Antitumor activity against human A549 cells after 24 hrs by MTT assay, ED50=26μM 17482824
N548 Cell death rescue assay Cell death rescue in rat striatal neuron N548 mutant cells, EC50=0.5μM 17726098
ASH neuronal cells Cell death rescue assay Cell death rescue of ASH neuronal cells in Caenorhabditis elegans Huntington's disease model, EC50=2μM 17726098
neuronal cells Cell death rescue assay Cell death rescue of neuronal cells in drosophila Huntington's disease model, EC50=10μM 17726098
N548 Toxicity assay Toxicity in rat striatal neuronal N548 mutant cells, TC50=40μM 17726098
N548 Cell death rescue assay Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 7 activation by Western blotting 17726098
N548 Activation of ERK in 10 uM Activation of ERK in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting 17726098
N548 Activation of AKT in 10 uM Activation of AKT in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting 17726098
N548 Activation of ERK in 50 uM Activation of ERK in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting 17726098
N548 Activation of AKT in 50 uM Activation of AKT in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting 17726098
N548 Activation of AKT in 10 uM 6 hrs Activation of AKT in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM after 6 hrs by Western blotting 17726098
N548 Cell death rescue assay Cell death rescue in rat striatal neuronal N548 mutant cells by calcein acetoxymethyl ester assay 17726098
N548 Cell death rescue assay 10 uM 7 days Cell death rescue in rat striatal neuronal N548 mutant cells analysed every day for 7 days at 10 uM by trypan blue dye-exclusion assay 17726098
N548 Cell death rescue assay Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 3 activation by Western blotting 17726098
N548 Cell death rescue assay 10 uM Cell death rescue in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM 17726098
U373 MG Antiproliferative assay 72 hrs Antiproliferative activity against human U373 MG cells after 72 hrs 18513974
T47D Function assay 0.001 to 0.1 uM Inhibition of cellular respiration in human T47D cells assessed as rate of oxygen consumption at 0.001 to 0.1 uM 21875114
T47D Function assay 48 hrs Reduction in ATP level in human T47D cells after 48 hrs by HPLC-UV analysis 21875114
T47D Function assay 0.01 to 0.1 uM 72 hrs Reduction in ATP level in human T47D cells at 0.01 to 0.1 uM after 72 hrs by HPLC-UV analysis 21875114
T47D Function assay 0.1 to 1 uM 30 mins Inhibition of mTOR in human T47D cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting 21875114
T47D Function assay 0.1 to 1 uM 30 mins Inhibition of mTOR in human T47D cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting 21875114
MDA-MB-231 Function assay 0.1 to 1 uM 30 mins Inhibition of mTOR in human MDA-MB-231 cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting 21875114
MDA-MB-231 Function assay 0.1 to 1 uM 30 mins Inhibition of mTOR in human MDA-MB-231 cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting 21875114
MDA-MB-231 Function assay 0.01 to 0.1 uM 48 hrs Reduction in ATP level in human MDA-MB-231 cells at 0.01 to 0.1 uM after 48 hrs by HPLC-UV analysis 21875114
697 Cytotoxicity assay 72 hrs Cytotoxicity against human 697 cells after 72 hrs by MTS assay, IC50=0.3μM 23895019
697 Cytotoxicity assay 48 hrs Cytotoxicity against human 697 cells after 48 hrs by MTS assay, IC50=0.3μM 23895019
HT-29 Cytotoxicity assay 72 hrs Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay, IC50=0.3μM 23895019
Hepa-1c1c7 Cytotoxicity assay Cytotoxicity against mouse Hepa-1c1c7 cells, IC50=0.7μM 23895019
Hepa1c1c7 Function assay Induction of quinone reductase in mouse Hepa1c1c7 cells, CD=0.8μM 23895019
Raji Cytotoxicity assay 72 hrs Cytotoxicity against human Raji cells after 72 hrs by MTS assay, IC50=1.1μM 23895019
Raji Cytotoxicity assay 48 hrs Cytotoxicity against human Raji cells after 48 hrs by MTS assay, IC50=4.2μM 23895019
TA3/Ha Function assay 12 uM Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 12 uM by spectrofluorometer analysis 24568614
L5178Y Cytotoxicity assay Cytotoxicity against mouse L5178Y cells by MTT assay, IC50=0.3μM 28075580
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells 29435139
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
Rh41 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB-EBc1 cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells 29435139
fibroblast cells qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for control Hh wild type fibroblast cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells 29435139
HepG2 Cytotoxicity assay 24 hrs Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=1.52μM 30613327
DLD1 Function assay Inhibition of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as reduction in oxygen consumption rate by seahorse XFe96 analyser based assay 31774672
Click to View More Cell Line Experimental Data

Biological Activity

Description Rotenone is a botanical insecticide that is an inhibitor of mitochondrial electron transport. Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase with IC50 of 28.8 nM and 5.1 nM, respectively. Rotenone induces apoptosis through enhancing mitochondrial reactive oxygen species production.
In vitro
In vitro

Rotenone inhibits NADH/DB oxidoreductase and NADH oxidase, and induces apoptosis through enhancing mitochondrial reactive oxygen species production.[1]
Cell Research Cell lines Naïve CD8+ T cells
Concentrations 0.1 µM
Incubation Time 1 day
Method

OCR of CD8+ T cells are analyzed under basal conditions and after sequential injections of 1 μM oligomycin A, 2 μM carbonyl cyanide p-trifluoromethoxyphenylhydrazone, and 0.1 μM rotenone with 1 μM antimycin A. ECAR is analyzed under basal conditions and after sequential injections of 10 mM glucose, 1 μM oligomycin A, and 50 mM 2-deoxyglucose.
Experimental Result Images Methods Biomarkers Images PMID
Western blot sestrin2 p-AMPK / AMPK p-mTOR / mTOR / p-S6K1 / S6K1 / p-4EBP1 / 4EBP1 p-CaMKII / CaMKII 26031332
Immunofluorescence p65 activated caspase-1 23977201
Growth inhibition assay Cell viability 27045591
In Vivo
In vivo

Rotenone, a mitochondrial toxin, induce the up-regulation of alpha-synuclein (alpha-syn) in PD models.[2]

Chemical Information & Solubility

Molecular Weight 394.42 Formula

C23H22O6
CAS No. 83-79-4 SDF Download Rotenone SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 78 mg/mL ( (197.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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