Home PI3K/Akt/mTOR PDPK1 activator PS-48 For research use only.

PS-48

PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).

PS-48 Chemical Structure

PS-48 Chemical Structure

CAS No. 1180676-32-7

Purity & Quality Control

Batch: S758601 DMSO]57 mg/mL]false]Ethanol]57 mg/mL]false]Water]Insoluble]false Purity: 99.73%
99.73

PS-48 Related Products

Biological Activity

Description PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).
Targets
PDPK1 [1]
In vitro
In vitro

The PDK1 agonist PS48 is able to promote the osteogenic ability of MC3T3-E1 cells treated with dexamethasone. The protein levels of p-AKT and p-mTOR are increased in MC3T3-E1 cells treated with dexamethasone after PS48 treatment.[2]
Cell Research Cell lines MC3T3-E1 cells
Concentrations 5 μM
Incubation Time --
Method

MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10−7 M dexamethasone supplemented with or without PS48 (5 μM).
In Vivo
In vivo

In vivo, the PDK1 agonist PS48 can maintain the bone mass of mice treated with dexamethasone.[2]
Animal Research Animal Models 8-week-old male C57BL/6J mice
Dosages --
Administration --

Chemical Information & Solubility

Molecular Weight 286.75 Formula

C17H15ClO2
CAS No. 1180676-32-7 SDF --
Smiles C1=CC=C(C=C1)C(=CC(=O)O)CCC2=CC=C(C=C2)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 57 mg/mL ( (198.77 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 57 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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