Home Immunology & Inflammation cGAS inhibitor PF-06928215 For research use only.

PF-06928215

PF-06928215 is a high affinity inhibitor of cyclic GMP-AMP synthase (cGAS) with an IC50 of 4.9 μM.

PF-06928215 Chemical Structure

PF-06928215 Chemical Structure

CAS No. 2378173-15-8

Purity & Quality Control

PF-06928215 Related Products

Biological Activity

Description PF-06928215 is a high affinity inhibitor of cyclic GMP-AMP synthase (cGAS) with an IC50 of 4.9 μM.
Targets
cGAS [1]
4.9 μM
In vitro
In vitro

PF-06928215, a high affinity inhibitor of Cyclic GMP-AMP synthase (cGAS), has no activity in cellular cGAS assays measuring dsDNA-induced IFN-beta expression via a luciferase reporter. PF-06928215 has minimal effect on cell viability.<sup><a class="sref" href="#s_ref">[1]</a></sup>
Cell Research Cell lines THP-1 Dual cells
Concentrations 0.01-100 μM
Incubation Time 1 h
Method

THP-1 Dual cells are pretreated with various concentrations of PF-06928215 (0.01-100 μM) for 1 hr followed by stimulation with salmon sperm DNA for 12 hrs. Media is collected and analyzed for luciferase signal, and cell viability is analyzed.

Chemical Information & Solubility

Molecular Weight 380.40 Formula

C20H20N4O4
CAS No. 2378173-15-8 SDF --
Smiles OC(=O)C1CCCCC1NC(=O)C2=C3N=C(C=C(O)[N]3N=C2)C4=CC=CC=C4
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 76 mg/mL ( (199.78 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy PF-06928215 | PF-06928215 supplier | purchase PF-06928215 | PF-06928215 cost | PF-06928215 manufacturer | order PF-06928215 | PF-06928215 distributor