Home Neuronal Signaling GluR antagonist NBQX (FG9202) For research use only.

NBQX (FG9202)

Synonyms: NNC 079202

NBQX (FG9202, NNC 079202) is a highly selective and competitive antagonist of AMPA receptor (AMPAR) with anti-epileptic effect.

NBQX (FG9202) Chemical Structure

NBQX (FG9202) Chemical Structure

CAS No. 118876-58-7

Purity & Quality Control

Batch: S968601 DMSO]67 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 98.86%
98.86

NBQX (FG9202) Related Products

Signaling Pathway

GluR Signaling Pathways

GluR Signaling Pathway

Biological Activity

Description NBQX (FG9202, NNC 079202) is a highly selective and competitive antagonist of AMPA receptor (AMPAR) with anti-epileptic effect.
Targets
AMPAR [1]
In vitro
In vitro

NBQX is highly selective in vitro. The IC50 value for inhibition of AMPA-evoked inward currents is 0.4 μM for NBQX. While NBQX inhibits NMDA-induced currents with IC50 value of 60 μM.[2]
Cell Research Cell lines Mouse neocortical neurones
Concentrations 0.1 μM - 100 μM
Incubation Time --
Method

Cells are continuously superfused with extracellular saline at a rate of 2.5 ml/min. After giga-seal formation the whole cell configuration is obtained by suction.The holding potential is - 60 mV and at the start of each experiment the current is measured continuously for 30 s to ensure a stable baseline. Agonist-containing solutions are delivered to the chamber through a custom-made gravity-driven flowpipe, the tip of which is placed approximately 50 μm from the cell. Application is triggered when the tubing connected to the flowpipe is compressed by a valve controlled by the Pulse software. In general, agonists are applied for 1.5 or 3 s every 45 s. The sample interval during application is 600 μs. After stable responses are obtained, the extracellular saline as well as the agonist-containing solution are replaced by solutions containing NBQX to be tested. NBQX is present until responses of a repeatable amplitude are achieved. Currents are measured at the plateau phase of the responses just before deactivation.
In Vivo
In vivo

Rats are intraperitoneally (i.p.) injected with pentylenetetrazole (PTZ, 50 mg/kg) for 28 consecutive days to establish chronic epilepsy models. Subsequently, NBQX (20 mg/kg, i.p.) is injected for 3 days for the observation of behavioral measurements of epilepsy. NBQX treatment normalizes perineuronal nets (PNNs), tenascin-R, aggrecan and neurocan levels. NBQX is sufficient to decrease seizures through increasing the latency to seizures, decrease the duration of seizure onset, and reduce the scores for the severity of seizures.[1]
Animal Research Animal Models Male Wistar rats
Dosages 20 mg/kg
Administration IP

Chemical Information & Solubility

Molecular Weight 336.28 Formula

C12H8N4O6S
CAS No. 118876-58-7 SDF --
Smiles N[S](=O)(=O)C1=C2C(=CC=C1)C3=C(NC(=O)C(=O)N3)C=C2[N+]([O-])=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 67 mg/mL ( (199.23 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy NBQX (FG9202) | NBQX (FG9202) supplier | purchase NBQX (FG9202) | NBQX (FG9202) cost | NBQX (FG9202) manufacturer | order NBQX (FG9202) | NBQX (FG9202) distributor