Home Hippo TEAD inhibitor MYF-01-37 For research use only.

MYF-01-37

MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.

MYF-01-37 Chemical Structure

MYF-01-37 Chemical Structure

CAS No. 2416417-65-5

Purity & Quality Control

MYF-01-37 Related Products

Biological Activity

Description MYF-01-37 is a novel covalent inhibitor of TEAD targeting Cys380 when incubated with the TEAD2 protein.
Targets
TEAD [1]
In vitro
In vitro

MYF-01-37 is developed as a covalent binder to TEAD, targeting Cys380 when incubated with the TEAD2 protein (C359 in TEAD1). Pretreating cells with MYF-01-37 leads to loss of direct TEAD pull-down by biotin-MYF-01-037 from whole-cell lysate, confirming MYF-01-037 binding to TEAD in cells.[1]
Cell Research Cell lines PC9 cells
Concentrations 10 μM, 1 μM
Incubation Time 6 h
Method

Cells are treated with test compounds for 6 h at the indicated doses. Total cell lysates are prepared using pulldown buffer. To pull down TEADs, 1 mg of total protein is combined with biotinylated MYF-01-37 at 1 μM and rotated at 4 ℃ for 6 hrs followed by RT for 1 h. Subsequently, streptavidin agarose resin (30 ml 50% slurry) is added and samples rotated for another 2 h at 4 ℃. The resin is subsequently washed 3× with pulldown buffer, and TEADs released from the resin by boiling for 10 min in 2× gel loading buffer and resolved by western blotting. As a loading control, 25 mg of total protein is used.

Chemical Information & Solubility

Molecular Weight 298.3 Formula

C15H17F3N2O
CAS No. 2416417-65-5 SDF --
Smiles CC1(CCN(C1)C(=O)C=C)NC2=CC=CC(=C2)C(F)(F)F
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 60 mg/mL ( (201.13 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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