Home Microbiology HBV inhibitor Morphothiadin For research use only.

Morphothiadin

Synonyms: GLS4

Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. Morphothiadin (GLS4), a nucleoside analogs that target the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.

Morphothiadin Chemical Structure

Morphothiadin Chemical Structure

CAS No. 1092970-12-1

Purity & Quality Control

Batch: E009401 DMSO]100 mg/mL]false]Ethanol]12 mg/mL]false]Water]Insoluble]false Purity: 99.87%
99.87

Morphothiadin Related Products

Biological Activity

Description Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. Morphothiadin (GLS4), a nucleoside analogs that target the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.
Targets
HBV [1]
(in the Southern blotting of analysis of replicative intermediate of HBV)
12 nM
In vitro
In vitro

In vitro, Morphothiadin (GLS4) is significantly less toxic for primary human hepatocytes, and inhibits virus accumulation in the supernantant of HepAD38 cells and HBV replicative forms in the liver significantly, also strongly inhibits core gene expression.[1];
Cell Research Cell lines HepAD38 cells
Concentrations 25, 50, 100, 200, 400 nM
Incubation Time 7 days
Method

5×106 HepAD38 cells were seeded into each well of 6-well plates, and the wells were then treated with GLS4 for 7 days. Fresh medium and drug were added daily. Cell supernatants were assayed for the levels of HBV DNA by real-time PCR.
In Vivo
In vivo

For nude mice inoculated with HepAD38 cells, treatment of with GLS4 shows strong and sustained suppression of virus DNA to about the same extents both during and after treatment, and reduces the levels of intracellular core antigen in the tumors.[1]
Animal Research Animal Models Nude mice inoculated with HepAD38 cells
Dosages 3.75 mg/kg
Administration o.g.

Chemical Information & Solubility

Molecular Weight 509.39 Formula

C21H22BrFN4O3S
CAS No. 1092970-12-1 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (196.31 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 12 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Morphothiadin | Morphothiadin supplier | purchase Morphothiadin | Morphothiadin cost | Morphothiadin manufacturer | order Morphothiadin | Morphothiadin distributor