Home Transmembrane Transporters NCX inhibitor - JNK activator - Autophagy inhibitor KB-R7943 mesylate For research use only.

KB-R7943 mesylate

KB-R7943 mesylate is a widely used inhibitor of the reverse Na+/Ca2+ exchanger (NCX(rev)) with IC50 of 5.7 μM. KB-R7943 mesylate promotes prostate cancer cell death by activating the JNK pathway and blocking autophagic flux.

KB-R7943 mesylate Chemical Structure

KB-R7943 mesylate Chemical Structure

CAS No. 182004-65-5

Purity & Quality Control

Batch: S464301 DMSO]86 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.86%
99.86

KB-R7943 mesylate Related Products

Biological Activity

Description KB-R7943 mesylate is a widely used inhibitor of the reverse Na+/Ca2+ exchanger (NCX(rev)) with IC50 of 5.7 μM. KB-R7943 mesylate promotes prostate cancer cell death by activating the JNK pathway and blocking autophagic flux.
Targets
NCX(rev) [1]
(Cell-free assay)
5.7 μM
In vitro
In vitro

KB-R7943 inhibits the reverse NCX more potently than its forward mode, thus occludes NCX-mediated Ca2+ extrusion and elevates intracellular Ca2+ level of melanoma.[3]
Cell Research Cell lines Flua-4 AM loaded A2058 melanoma cells
Concentrations 25 or 50 µM
Incubation Time 5 min
Method

Cells were plated in 96-well cell culture plates at 6000 per well and cultured overnight. Cells were treated with test reagents for 24–72 h according to different requirements. Cells were cultured in 96-well plates at 6000 per well and loaded with the cell membrane permeable Ca2+ dye Fluo-4 AM (5 μM) in HEPES-buffered solution for 40 min. The HEPES solution was supplemented with 1% FBS at pH 7.4.

Chemical Information & Solubility

Molecular Weight 427.5 Formula

C17H21N3O6S2
CAS No. 182004-65-5 SDF --
Smiles C[S](O)(=O)=O.NC(=N)SCCC1=CC=C(OCC2=CC=C(C=C2)[N+]([O-])=O)C=C1
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 86 mg/mL ( (201.16 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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