Home Metabolism PDE inhibitor IBMX For research use only.

IBMX

Synonyms: Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine

IBMX (Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine) is a nonspecific inhibitor of phosphodiesterase (PDE) with IC50 values of 6.5±1.2, 26.3±3.9 and 31.7±5.3 μM for PDE3, 4 and 5 respectively. It may enhance the intracellular cAMP levels and also acts as an adenosine (A1) receptor antagonist.

IBMX Chemical Structure

IBMX Chemical Structure

CAS No. 28822-58-4

Purity & Quality Control

IBMX Related Products

Signaling Pathway

PDE Signaling Pathways

PDE Signaling Pathway

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
adipocytes, heart and brain cells Function assay Affinity constant for inhibition of A1 receptor control of adenylate cyclase in adipocytes, heart and brain cells, Affinity constant=2μM 6279840
adipocytes, heart and brain cells Function assay Affinity constant for A2 receptor control of adenylate cyclase in adipocytes, heart and brain cells, Affinity constant=5μM 6279840
3T3L1 Function assay 0.25 mM 48 hrs Stimulation of lipolysis in mouse 3T3L1 cells at 0.25 mM after 48 hrs by glycerol release assay 24597820
BL21(DE3) pLysS Function assay Inhibition of human His-tagged PDE3B catalytic domain expressed in Escherichia coli BL21(DE3) pLysS cells, IC50=0.242μM 26908025
Sf21 Function assay Inhibition of human C-terminal His6-tagged PDE2A1 (215 to 900 residues) expressed in sf21 cells, IC50=4μM 26908025
BL21-CodonPlus Function assay Inhibition of recombinant human His6-tagged PDE5A (535 to 786 residues)/PDE6C expressed in Escherichia coli BL21-CodonPlus cells using [3H]cGMP as substrate, Ki=8.5μM 26908025
Sf9 Function assay 10 mins Inhibition of human GST-tagged PDE4B expressed in baculovirus infected Sf9 insect cells using cAMP as substrate after 10 mins by PDE-Glo Phosphodiesterase Assay, IC50=28.2μM 29407965
Click to View More Cell Line Experimental Data

Biological Activity

Description IBMX (Isobutylmethylxanthine, 1-Methyl-3-Isobutylxanthine) is a nonspecific inhibitor of phosphodiesterase (PDE) with IC50 values of 6.5±1.2, 26.3±3.9 and 31.7±5.3 μM for PDE3, 4 and 5 respectively. It may enhance the intracellular cAMP levels and also acts as an adenosine (A1) receptor antagonist.
Targets
PDE3 [1] PDE4 [1] PDE5 [1]
6.5 μM 26.3 μM 31.7 μM
In vitro
In vitro

IBMX rescues PGE2-induced phosphorylation of AKT inhibited in cells from the EP4LysM mice, which protects against cAMP degradation.[3]
Cell Research Cell lines Bone marrow macrophages of Ep4fl/fl and Ep4LysM mice
Concentrations 1 mM
Incubation Time 3 h
Method

The cells are treated either with osteoclastogenic media (10 ng/mL M-CSF and 50 ng/mL RANKL) alone, with PGE2 (100 nM), or PGE2 with IBMX (1 mM) for 3 h. A BCA Protein Assay Kit is used to determine the protein concentration.
In Vivo
In vivo

IBMX decreases the liver glycogen storage in both species of mice and hyperglycemic rat.[4]
Animal Research Animal Models Male mice (25-35 g), adult Wistar rats (250-350 g) of hyperglycemic model
Dosages 1 mg/kg dosage twice a day
Administration s.c.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02807415 Completed
Cystic Fibrosis
Hannover Medical School|Heidelberg University|University of Giessen
 June 1 2016 --
NCT03652090 Completed
Cystic Fibrosis
Institut National de la Santé Et de la Recherche Médicale France|ABCF2
 September 1 2010 --

Chemical Information & Solubility

Molecular Weight 222.24 Formula

C10H14N4O2
CAS No. 28822-58-4 SDF --
Smiles CC(C)CN1C2=C(C(=O)N(C1=O)C)NC=N2
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 45 mg/mL ( (202.48 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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