Home Cytoskeletal Signaling Kinesin inhibitor H-Cys(Trt)-OH For research use only.

H-Cys(Trt)-OH

Synonyms: L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine, S-Trityl-L-cysteine

H-Cys(Trt)-OH (L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine,S-Trityl-L-cysteine) is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.

H-Cys(Trt)-OH Chemical Structure

H-Cys(Trt)-OH Chemical Structure

CAS No. 2799-07-7

Purity & Quality Control

Batch: S552201 DMSO]5 mg/mL]false]]]false]]]false Purity: 98%
98

H-Cys(Trt)-OH Related Products

Signaling Pathway

Kinesin Signaling Pathways

Kinesin Signaling Pathway

Biological Activity

Description H-Cys(Trt)-OH (L-Cys(Trt)-OH, H-L-Cys(Trt)-OH, S-Tritylcysteine,S-Trityl-L-cysteine) is a specific inhibitor of Eg5 that inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM.
Targets
Eg5 [1]
In vitro
In vitro

In cell-based assays, H-Cys(Trt)-OH (S-trityl-l-cysteine) does not prevent cell cycle progression at the S or G2 phases but inhibits both separation of the duplicated centrosomes and bipolar spindle formation, thereby blocking cells specifically in the M phase of the cell cycle with monoastral spindles. Following removal of S-trityl-l-cysteine, mitotically arrested cells exit mitosis normally. In vitro, S-trityl-l-cysteine targets the catalytic domain of Eg5 and inhibits Eg5 basal and microtubule-activated ATPase activity as well as mant-ADP release. It is a tight binding inhibitor and inhibits Eg5-driven microtubule sliding velocity in a reversible fashion with an IC50 of 500 nM[1].
Cell Research Cell lines HeLa cells and U2OS cells
Concentrations 5 μM
Incubation Time 0, 24, 48, 72 h
Method

HeLa cells and U2OS cells were analyzed by FACScan analysis following incubation with STLC (5 μM) at the indicated times.

Chemical Information & Solubility

Molecular Weight 363.47 Formula

C22H21NO2S
CAS No. 2799-07-7 SDF --
Smiles C1=CC=C(C=C1)C(C2=CC=CC=C2)(C3=CC=CC=C3)SCC(C(=O)O)N
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 5 mg/mL ( (13.75 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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