Home Transmembrane Transporters Amino acid transporter inhibitor GPNA (L-γ-Glutamyl-p-nitroanilide) For research use only.

GPNA (L-γ-Glutamyl-p-nitroanilide)

GPNA (L-γ-Glutamyl-p-nitroanilide) is a potent and selective inhibitor of the glutamine (Gln) transporter ASCT2(SLC1A5) with Ki of 55 µM.

GPNA (L-γ-Glutamyl-p-nitroanilide) Chemical Structure

GPNA (L-γ-Glutamyl-p-nitroanilide) Chemical Structure

CAS No. 67953-08-6

Purity & Quality Control

GPNA (L-γ-Glutamyl-p-nitroanilide) Related Products

Biological Activity

Description GPNA (L-γ-Glutamyl-p-nitroanilide) is a potent and selective inhibitor of the glutamine (Gln) transporter ASCT2(SLC1A5) with Ki of 55 µM.
Targets
ASCT2 [1]
(Cell-free assay)
55 μM(Ki)
In vitro
In vitro

GPNA leads to a significant decrease in endometrial cancer cell growth and spheroid cross-sectional area.[1]
Cell Research Cell lines Endometrial cancer cell line
Concentrations 1 mM
Incubation Time 24 h
Method

Cells (1–2 × 105) are cultured for 24 h followed by treatment where stated with vehicle or 1 mm GPNA for 24 h. And then staining for flow cytometric analysis is performed. MTT assays are conducted using 100–250 cells per well in a 96-well plate. Fresh media with vehicle or 1 mm GPNA are applied every two days.
In Vivo
In vivo

EGFRAS-GPNA (EGFRAS-specific GPNA oligomers) is taken-up by several tissues including the xenograft tumor. Systemic administration of EGFRAS-GPNA induces antitumor effects in HNSCC xenografts, with similar efficacies as the FDA-approved EGFR inhibitors: cetuximab and erlotinib. EGFRAS-GPNA effectively againsts the constitutively active EGFR vIII mutant implicated in cetuximab resistance.

Chemical Information & Solubility

Molecular Weight 303.7 Formula

C11H14ClN3O5
CAS No. 67953-08-6 SDF --
Smiles C1=CC(=CC=C1NC(=O)CCC(C(=O)O)N)[N+](=O)[O-].Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 5 mg/mL ( (16.46 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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