Home Apoptosis OXPHOS inhibitor - ATPase inhibitor FCCP For research use only.

FCCP

Synonyms: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone

FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.

FCCP Chemical Structure

FCCP Chemical Structure

CAS No. 370-86-5

Purity & Quality Control

FCCP Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
T47D Function assay Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM 20929261
T47D Function assay Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM 20929261
T47D Function assay 1 to 10 uM Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA 20929261
T47D Function assay 0.3 uM 15 mins Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay 20929261
MDA-MB-231 Cytotoxicity assay 1 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM 23245650
MDA-MB-231 Cytotoxicity assay 0.1 to 3 uM Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor 23245650
MDA-MB-231 Function assay 0.3 uM Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM 23245650
T47D Function assay 0.1 to 3 uM Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM 22938093
T47D Function assay 10 to 30 uM Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin 22938093
T47D Function assay 0.3 to 1 uM Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin 22938093
Hep3B Function assay 10 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin 22938093
Hep3B Function assay 1 uM Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin 22938093
T47D Function assay 3 uM 15 to 20 mins Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay 22938093
TA3/Ha Function assay 6 uM Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis 24568614
SH-SY5Y Function assay 10 uM 5 mins Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay 25265024
SH-SY5Y Function assay 10 uM 10 mins Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay 25265024
T47D Function assay 0.3 uM 3 to 12 mins Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay 26637046
T47D Function assay 0.3 uM 30 mins Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy 26637046
T47D Function assay 0.3 uM Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate 26637046
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method 28233680
HCT116 Function assay 2 uM 30 mins Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method 28233680
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay 31774672
LS174T Function assay 1 uM Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
DLD1 Function assay 1 uM Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay 31774672
HEK293 Function assay 1 to 3 uM Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay 31774672
KOPN8 Function assay 10 uM 0.3 hrs Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis 31084028
HepG2 Function assay Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM ChEMBL
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Biological Activity

Description FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a potent uncoupler of oxidative phosphorylation in mitochondria that disrupts ATP synthesis by transporting protons across cell membranes.
Targets
OXPHOS [2] ATP synthase [2]
In vitro
In vitro

FCCP treatment induces a very rapid 2-fold increase in intracellular Ca2+ concentration that is accompanied by a strong protein synthesis rate inhibition. The translation inhibition correlates with an increased phosphorylation of the α subunit of eIF2 (eIF2α) and a 1.7-fold increase in the double-stranded RNA-dependent protein kinase activity[1].

FCCP treatment also mildly decreases ATP and reactive oxygen species levels. It increases the expression of mitochondrial genes such as Tfam and COXIV while inducing morphological features of quiescent mouse HSCs and abrogating TGF-β signal transduction[2].
Cell Research Cell lines PC12 cells
Concentrations 30 μM
Incubation Time 30 min, 1h, 2h
Method

Protein synthesis rate is assayed in 24-mm diameter multi-well dishes with fresh medium containing 0.175 Ci/mmol of [3H]methionine (200 μM), for 30 min at 37°C. PC12 cells are treated with FCCP for different period of times.
In Vivo
In vivo

FCCP significantly reduces mitochondrial membrane potential and ATP production in 8-cell mouse embryos and the number of inner cell mass cells within blastocysts with unchanged blastocyst development. This perturbed embryonic mitochondrial function is concomitant with reduced birth weight in female offspring following embryo transfer, which persists until weaning. Although FCCP-treated males also exhibits reduced glucose tolerance as female, but their insulin sensitivity and adiposity gain between 4 and 14 weeks is unchanged. Reducing mitochondrial function and, thus, decreasing ATP output in the precompacting embryo can influence offspring phenotype[3].
Animal Research Animal Models Female C57BL/6 mice
Dosages 1 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 254.17 Formula

C10H5F3N4O
CAS No. 370-86-5 SDF Download FCCP SDF
Smiles C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 6 mg/mL ( (23.6 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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