Home Methylation FTO inhibitor FB23 For research use only.

FB23

FB23 is a potent and selective inhibitor of FTO demethylase with IC50 of 60 nM. FB23 directly binds to FTO and selectively inhibits FTO's mRNA N6-methyladenosine (m6A) demethylase activity.

FB23 Chemical Structure

FB23 Chemical Structure

CAS No. 2243736-35-6

Purity & Quality Control

FB23 Related Products

Biological Activity

Description FB23 is a potent and selective inhibitor of FTO demethylase with IC50 of 60 nM. FB23 directly binds to FTO and selectively inhibits FTO's mRNA N6-methyladenosine (m6A) demethylase activity.
Targets
FTO demethylase [1]
(Cell-free assay)
60 nM
In vitro
In vitro

FB23 treatment inhibits acute myeloid leukemia (AML) cells proliferation with IC50 values of 44.8 μM, 23.6 μM for NB4 and MONOMAC6 AML cells. FB23 treatment causes the significant suppression of MYC targets, E2F targets, and G2M checkpoint signal cascades, which may contribute to the inhibitory effects of FTO inhibitors and FTO KD on cell cycle and proliferation. FB23 treatments activates apoptosis and p53 pathways.[1]
Cell Research Cell lines AML cell lines
Concentrations 1-50 μM
Incubation Time 24 h, 48 h, 72 h, 96 h
Method

AML cells are seeded and treated with DMSO or FB23 for 24 h, 48 h, 72 h, or 96 h. The cell proliferations are determined with CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. AML cells are treated with DMSO or 5 μM FB23-2 for 72 hr for dot blot assay.
In Vivo
In vivo

Pharmacological inhibition of FTO by FB23-2 substantially suppresses leukemia progression and prolongs survival.[1]

Chemical Information & Solubility

Molecular Weight 377.22 Formula

C18H14Cl2N2O3
CAS No. 2243736-35-6 SDF --
Smiles CC1=C(C(=NO1)C)C2=CC(=C(NC3=CC=CC=C3C(O)=O)C(=C2)Cl)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 38 mg/mL ( (100.73 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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