Home Neuronal Signaling GluR inhibitor Evans Blue For research use only.

Evans Blue

Synonyms: Direct Blue 53,C.I.23860

Evans Blue (Direct Blue 53,C.I.23860) is a potent inhibitor of the uptake of L-glutamate into synaptic vesicles, also an AMPA/kainate receptor antagonist.

Evans Blue Chemical Structure

Evans Blue Chemical Structure

CAS No. 314-13-6

Purity & Quality Control

Evans Blue Related Products

Signaling Pathway

GluR Signaling Pathways

GluR Signaling Pathway

Biological Activity

Description Evans Blue (Direct Blue 53,C.I.23860) is a potent inhibitor of the uptake of L-glutamate into synaptic vesicles, also an AMPA/kainate receptor antagonist.
Targets
AMPA/kainate receptor [7] vesicular glutamate uptake [5]
87 nM
In vitro
In vitro

Evans Blue is found to be inhibiting DNA binding of NF-κB at a low concentration of 100 μM[1]. It has proven over the years to be a dependable stain for microscopic determination of cell death[2]. Evans Blue is also a known blocker of a subset of a-amino-3-hydroxy-5-methyl-isoxazole/kainate receptors (IC50=355 nM) for the subunit combination GluR1,2[5]. Evans blue is the first known δ-subunit-specific antagonist of ENaC and activates large-conductance Ca2+-activated K+ channels in sheep bladder myocytes and cultured endothelial cells of human umbilical veins[5].
In Vivo
In vivo

Evans blue pretreatment could inhibit the mast cells degranulation and that may be the main mechanism for prevent animals from C48/80-triggered anaphylaxis and systemic inflammation[4]. Evans blue modulates the non-N-methyl-d-aspartate receptor in rat thalamic neurons, blocks the P2X-purinergic receptor in rat vas deferens, and inhibits the glutamate transporter in rat brain synaptic vesicles[6].
Animal Research Animal Models CD hairless rats (Charles River)
Dosages 100 mg/kg
Administration i.p

Chemical Information & Solubility

Molecular Weight 960.81 Formula

C34H24N6O14S4.4Na
CAS No. 314-13-6 SDF Download Evans Blue SDF
Smiles CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+]
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (104.07 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble


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In vivo
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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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