Home DNA Damage/DNA Repair Topoisomerase inhibitor Enoxacin For research use only.

Enoxacin

Synonyms: AT-2266, CI919, Pd107779, NSC 629661

Enoxacin (AT-2266, CI919, Pd107779, NSC 629661) is an oral broad-spectrum fluoroquinolone antibacterial agent by inhibiting bacterial DNA gyrase and topoisomerase IV, used to treat a wide variety of infections.

Enoxacin Chemical Structure

Enoxacin Chemical Structure

CAS No. 74011-58-8

Purity & Quality Control

Enoxacin Related Products

Signaling Pathway

Topoisomerase Signaling Pathways

Topoisomerase Signaling Pathway

Biological Activity

Description Enoxacin (AT-2266, CI919, Pd107779, NSC 629661) is an oral broad-spectrum fluoroquinolone antibacterial agent by inhibiting bacterial DNA gyrase and topoisomerase IV, used to treat a wide variety of infections.
Targets
Topoisomerase II [1] Topoisomerase IV [1]
In vitro
In vitro Enoxacin, a fluoroquinolone used as an antibacterial compound, enhances the production of miRNAs with tumor suppressor functions by binding to the miRNA biosynthesis protein TAR RNA-binding protein 2 (TRBP). [1] Enoxacin binds to the DNA active site and alters the breakage/reunion activity of the enzyme. Enoxacin stimulates cleavage of both relaxed and supercoiled forms of DNA in the absence of ATP, whereas CcdB induces cleavage only after many cycles of ATP-dependent breakage and reunion. [2] Enoxacin dose dependently reduces the number of osteoclasts differentiating in mouse marrow cultures stimulated with 1,25-dihydroxyvitamin D(3), as well as markers of osteoclast activity, and the number of resorption lacunae formed on bone slices. Enoxacin inhibits osteoclast formation at concentrations where osteoblast formation is not altered. [3] Enoxacin dose-dependently reduces the number of multinuclear cells expressing tartrate-resistant acid phosphatase (TRAP) activity produced by RANK-L-stimulated osteoclast precursors. Enoxacin directly inhibits osteoclast formation without affecting cell viability by a novel mechanism that involves changes in posttranslational processing and trafficking of several proteins with known roles in osteoclast function. [4] Enoxacin is able to decrease cell viability, induce apoptosis, cause cell cycle arrest, and inhibit the invasiveness of prostate cancer (PCa) cell lines. Enoxacin is also effective in restoring the global expression of miRNAs in prostate cancer (PCa) cell lines. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04840823 Completed
Amyotrophic Lateral Sclerosis
McGill University|Weizmann Institute of Science|Apotex Inc.
 March 26 2021 Phase 1|Phase 2

Chemical Information & Solubility

Molecular Weight 320.32 Formula

C15H17FN4O3
CAS No. 74011-58-8 SDF Download Enoxacin SDF
Smiles CCN1C=C(C(=O)C2=CC(=C(N=C21)N3CCNCC3)F)C(=O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 32 mg/mL ( (99.9 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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