TLQP-21 TFA

Catalog No.P1209 Purity: 99.90%

LQP-21 TFA (TLQP-21 Trifluoroacetate) is a VGF-derived peptide that activates the C3aR1 receptor through a folding-upon-binding mechanism.

Amino-acid Sequence

CAS No. 869988-94-3(free base)

Batch: Purity: 99.90%

99.90

Storage (From the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (39.26 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : 100 mg/mL

In vivo
Batch:

Add solvents to the product individually and in order.

Description

LQP-21 TFA (TLQP-21 Trifluoroacetate) is a VGF-derived peptide that activates the C3aR1 receptor through a folding-upon-binding mechanism.

Targets

C3aR1 receptor

In vitro
In vitro	TLQP-21 activates the cognate C3aR1 receptor leading to enhanced lipolysis in adipocytes and arginine 21 is essential for TLQP-21 lipolysis in 3T3L1 adipocytes.^[1]
Cell Research	Cell lines	3T3L1 adipocytes
	Concentrations	100 nM
	Incubation Time	3 h
	Method	3T3-L1 adipocytes differentiated on 6-well plates are first starved for 3 hours in KRH with 1% BSA, then incubate with TLQP-21 in the presence and absence of specific agonists/antagonists, suspended in KRH with 4% BSA. Following 3 hours of incubation at 37°C and 5% CO2, the media is collected and incubated at 60°C for 20 min to inactivate any residual enzymatic reaction activity. The cells are gathered for protein concentration or trypsinized for cell diameter and viability measurements.

References
[1] Cheryl Cero, et al. Structure . 2014 Dec 2;22(12):1744-1753.

References

Molecular Formula:	C₁₀₇H₁₇₀N₄₀O₂₆(TFA)
Molecular Weight:	2546.75
CAS No.:	869988-94-3(free base)
Appearance:	White Powder
Peptide Content (by %N):	≥80%
Single Impurity (by HPLC):	≤1.0%
Assay (By Anhydrous, Acetic Acid-free):	95.0~105.0%
MS:	Consistent
Packing:	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt):	3 years-20°C powder
Shipping:	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)