Doxorubicin

Catalog No.E2516 Batch:E251601

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Technical Data

Formula

C27H29NO11
Molecular Weight 543.52 CAS No. 23214-92-8
Solubility (25°C)* In vitro DMSO 100 mg/mL (183.98 mM)
Water 50 mg/mL (91.99 mM)
Ethanol Insoluble
In vivo (Add solvents to the product individually and in order)
Clear solution
saline
50.000mg/ml (91.99mM) Taking the 1 mL working solution as an example, add 50 mg of this product to 1 ml of physiological saline (0.9% NaCL solution), mix evenly to make it clear, The mixed solution should be used immediately for optimal results.
Homogeneous suspension
CMC-NA
≥5mg/ml Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Adriamycin (Doxorubicin, Hydroxydaunorubicin), a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent, inhibits topoisomerase II with an IC50 of 2.67 μM, thus stopping DNA replication, and induces apoptosis, also inhibits human DNA topoisomerase I with an IC50 of 0.8 μM.
Targets
topoisomerase I [3]
(in assay of DNA Topo I activity)		 topoisomerase II [1]
(in decatenation assay for topoisomerase II activity)
0.8 μM 2.67 μM

Protocol (from reference)

Selleck's Doxorubicin has been cited by 159 publications

CD36-mediated endocytosis of proteolysis-targeting chimeras [ Cell, 2025, S0092-8674(25)00386-1] PubMed: 40250420
Targeting ATAD3A Phosphorylation Mediated by TBK1 Ameliorates Senescence-Associated Pathologies [ Adv Sci (Weinh), 2025, 12(1):e2404109] PubMed: 39520088
Pristimerin Promotes Ubiquitination of HSPA8 and Activates the VAV1/ERK Pathway to Suppress TNBC Proliferation [ Adv Sci (Weinh), 2025, 12(10):e2413174] PubMed: 39813169
Smad1 Promotes Tumorigenicity and Chemoresistance of Glioblastoma by Sequestering p300 From p53 [ Adv Sci (Weinh), 2025, 12(4):e2402258] PubMed: 39629919
Construction of human pluripotent stem cell-derived testicular organoids and their use as humanized testis models for evaluating the effects of semaglutide [ Theranostics, 2025, 15(6):2597-2623] PubMed: 39990223
Dual role of p21 in regulating apoptosis and mitotic integrity in response to doxorubicin in colon cancer cells [ Cell Death Discov, 2025, 11(1):133] PubMed: 40175326
Genomic and Phenotypic Characterization of CHO 4BGD Cells with Quad Knockout and Overexpression of Two Housekeeping Genes That Allow for Metabolic Selection and Extended Fed-Batch Culturing [ Cells, 2025, 14(10)692] PubMed: 40422195
Downregulation of rRNA synthesis by BCL-2 induces chemoresistance in diffuse large B cell lymphoma [ iScience, 2025, 28(5):112333] PubMed: 40276769
Serum-derived extracellular vesicles mediate acquired multidrug resistance of MCF-7 breast cancer cells induced by chemotherapeutic drugs [ Biochem Pharmacol, 2025, 237:116923] PubMed: 40194604
The Role of Elacridar, a P-gp Inhibitor, in the Re-Sensitization of PAC-Resistant Ovarian Cancer Cell Lines to Cytotoxic Drugs in 2D and 3D Cell Culture Models [ Int J Mol Sci, 2025, 26(3)1124] PubMed: 39940891

RETURN POLICY
Selleck Chemical’s Unconditional Return Policy ensures a smooth online shopping experience for our customers. If you are in any way unsatisfied with your purchase, you may return any item(s) within 7 days of receiving it. In the event of product quality issues, either protocol related or product related problems, you may return any item(s) within 365 days from the original purchase date. Please follow the instructions below when returning products.

SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.