Cycloheximide

Catalog No.S7418 Batch:S741805

Technical Data

Formula	C₁₅H₂₃NO₄
Molecular Weight	281.35	CAS No.	66-81-9
Solubility (25°C)*	In vitro	DMSO	56 mg/mL (199.04 mM)
		Ethanol	56 mg/mL (199.04 mM)
		Water	15 mg/mL (53.31 mM)
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description	Cycloheximide, an antifungal antibiotic, is an eukaryote protein synthesis inhibitor with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively. Cycloheximide suppresses ferroptosis and inhibits autophagy.Cycloheximide (NSC-185) can be used to induce animal models of Dysmnesia.Solutions are unstable and should be fresh-prepared.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.
In vitro	Cyclohexamide, a protein synthesis inhibitor, reduces GSIV-induced host cell death by attenuating phosphatidylserine exposure and loss of ΔΨm from apoptosis/necrosis.^[1]
In vivo	Cycloheximide, a protein synthesis inhibitors, acts on memory by altering modulators of memory formation as a secondary consequence of the inhibition of protein synthesis.^[2]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines grouper fin cell line (GF-1) Concentrations 0.33 µg/ml Incubation Time 0-5 days Method Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.
Animal Study:^{^[2]}	Animal Models Male ICR mice Dosages 30mg/kg, 60mg/kg,120 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
grouper fin cell line (GF-1)
Concentrations
0.33 µg/ml
Incubation Time
0-5 days
Method
Monolayers of GF-1 cells (4.0 mL, 10⁵ cells/mL) on 60-mm Petri dishes are cultivated for at least 20 h, rinsed twice with phosphate buffered saline (PBS), treated with the protein synthesis inhibitor cycloheximide (CHX, 0.33 µg/mL) and ANT inhibitor BKA (20 µM) for 0 5 days, then infected with GSIV K1 strain (multiplicity of infection [m.o.i. ] = 5) for 0 5 days post-infection (dpi). At the end of the incubation period, each sample is removed from the medium and washed with PBS. Cells are then incubated for 10-15 min with 100 µL staining solution.

Animal Study:^{^[2]}

Animal Models
Male ICR mice
Dosages
30mg/kg, 60mg/kg,120 mg/kg
Administration
i.p.

References

[4] Santos DA, et al. Nucleic Acids Res. 2019;47(10):4974-4985.

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Selleck's Cycloheximide has been cited by 425 publications

VCP downstream metabolite glycerol-3-phosphate (G3P) inhibits CD8+T cells function in the HCC microenvironment [ Signal Transduct Target Ther, 2025, 10(1):26]	PubMed: 39848960
The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism [ Mol Cell, 2025, 85(3):638-651.e9]	PubMed: 39889707
OTUD5 Protects Dopaminergic Neurons by Promoting the Degradation of α-Synuclein in Parkinson's Disease Model [ Adv Sci (Weinh), 2025, 12(7):e2406700]	PubMed: 39721018
Glutathione reductase underlies the stability of mutant p53 by antagonizing protein glutathionylation [ Redox Biol, 2025, 81:103522]	PubMed: 39983342
Circ-0001283 Aggravates Cardiac Hypertrophy by Targeting Myosin Light Chain 3 Protein [ Research (Wash D C), 2025, 8:0626]	PubMed: 40007621
Hypoxia-induced degradation of FTO promotes apoptosis by unmasking RACK1-mediated activation of MTK1-JNK1/2 pathway [ J Adv Res, 2025, S2090-1232(25)00038-4]	PubMed: 39805423
FOXS1, frequently inactivated by promoter methylation, inhibited colorectal cancer cell growth by promoting TGFBI degradation through autophagy-lysosome pathway [ J Adv Res, 2025, S2090-1232(25)00056-6]	PubMed: 39864590
METTL14-mediated m6A modification of ZFP14 inhibits clear cell renal cell carcinoma progression via promoting STAT3 ubiquitination [ Clin Transl Med, 2025, 15(2):e70232]	PubMed: 39936533
VANGL2 alleviates inflammatory bowel disease by recruiting the ubiquitin ligase MARCH8 to limit NLRP3 inflammasome activation through OPTN-mediated selective autophagy [ PLoS Biol, 2025, 23(2):e3002961]	PubMed: 39899477
Deciphering transcription activity of mammalian early embryos unveils on/off of zygotic genome activation by protein translation/degradation [ Cell Rep, 2025, 44(1):115215]	PubMed: 39823229

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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