Gentisic acid

Synonyms: DHB, 2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Gentianic acid, Carboxyhydroquinone, Hydroquinonecarboxylic acid

Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.

Gentisic acid Chemical Structure

Gentisic acid Chemical Structure

CAS No. 490-79-9

Purity & Quality Control

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Biological Activity

Description Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.
In vitro
In vitro

Gentisic acid (GA) has been shown to act as an antioxidant under different types of physical and chemical stimulus. Gentisic acid efficiently protects lipids, proteins, SOD enzyme and human erythrocytes on gamma radiation exposure. It has been found to scavenge physiologically relevant hydroxyl and peroxyl radicals in pulse radiolysis studies[3].

Cell Research Cell lines HTC cells
Concentrations 0.08, 0.16, 0.8, 1.6, and 8 μg/mL
Incubation Time 24 h
Method

The Trypan Blue exclusion protocol is assessed in 24-well cell culture plates. In each well, 2.5×cells are seeded in 2 mL of complete culture medium with the following treatments: control (CO, 50 μL of culture medium, as negative control), cytotoxic agent doxorubicin (DXR, 10 μg/mL, as positive control), and Gentisic acid (0.08, 0.16, 0.8, 1.6, and 8 μg/mL). The cells are incubated for 24 h, trypsinized and then resuspended in 0.5 mL of culture medium. An aliquot of the cellular suspension (20 μL) is homogenized with Trypan Blue (0.2%) dye in a 1:1 ratio. The cells are quantified with a Neubauer chamber hemocytometer.

In Vivo
In vivo

Gentisic acid (GA) shows a potent protective effect against CP induced hepatotoxicity and genotoxicity. The protective effect of Gentisic acid can be attributed to its antioxidant properties, which enhance natural antioxidant enzymes and the cell membrane stabilizing property by inhibiting lipid peroxidation[2].

Animal Research Animal Models male Swiss albino mice
Dosages 50 and 100 mg/kg
Administration oral administration
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01015820 Completed
Pancreatic Cancer
Mayo Clinic|Northwestern University|National Cancer Institute (NCI)|National Institute for Biomedical Imaging and Bioengineering (NIBIB)
June 2010 Not Applicable

Chemical Information & Solubility

Molecular Weight 154.12 Formula

C7H6O4

CAS No. 490-79-9 SDF Download Gentisic acid SDF
Smiles C1=CC(=C(C=C1O)C(=O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 30 mg/mL ( (194.65 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 7.5 mg/mL


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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