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Gentisic acid

Name: Gentisic acid
Brand: Selleck Chemicals
SKU: S3799
Rating: 5 (1 reviews)

Synonyms: DHB, 2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Gentianic acid, Carboxyhydroquinone, Hydroquinonecarboxylic acid

Catalog No.S3799 Purity:99.96%

Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.

Gentisic acid Chemical Structure

CAS No. 490-79-9

Purity & Quality Control

Batch: Purity: 99.96%

99.96

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Biological Activity

Description	Gentisic acid (2,5-dihydroxybenzoic acid, 5-Hydroxysalicylic acid, Hydroquinonecarboxylic acid, DHB), an active metabolite of salicylic acid degradation, has a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.

In vitro
In vitro	Gentisic acid (GA) has been shown to act as an antioxidant under different types of physical and chemical stimulus. Gentisic acid efficiently protects lipids, proteins, SOD enzyme and human erythrocytes on gamma radiation exposure. It has been found to scavenge physiologically relevant hydroxyl and peroxyl radicals in pulse radiolysis studies^[3].
Cell Research	Cell lines	HTC cells
	Concentrations	0.08, 0.16, 0.8, 1.6, and 8 μg/mL
	Incubation Time	24 h
	Method	The Trypan Blue exclusion protocol is assessed in 24-well cell culture plates. In each well, 2.5×cells are seeded in 2 mL of complete culture medium with the following treatments: control (CO, 50 μL of culture medium, as negative control), cytotoxic agent doxorubicin (DXR, 10 μg/mL, as positive control), and Gentisic acid (0.08, 0.16, 0.8, 1.6, and 8 μg/mL). The cells are incubated for 24 h, trypsinized and then resuspended in 0.5 mL of culture medium. An aliquot of the cellular suspension (20 μL) is homogenized with Trypan Blue (0.2%) dye in a 1:1 ratio. The cells are quantified with a Neubauer chamber hemocytometer.

In Vivo
In vivo	Gentisic acid (GA) shows a potent protective effect against CP induced hepatotoxicity and genotoxicity. The protective effect of Gentisic acid can be attributed to its antioxidant properties, which enhance natural antioxidant enzymes and the cell membrane stabilizing property by inhibiting lipid peroxidation^[2].
Animal Research	Animal Models	male Swiss albino mice
	Dosages	50 and 100 mg/kg
	Administration	oral administration

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT01015820	Completed	Pancreatic Cancer	Mayo Clinic\|Northwestern University\|National Cancer Institute (NCI)\|National Institute for Biomedical Imaging and Bioengineering (NIBIB)	June 2010	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	154.12	Formula	C₇H₆O₄
CAS No.	490-79-9	SDF	Download Gentisic acid SDF
Smiles	C1=CC(=C(C=C1O)C(=O)O)O
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 30 mg/mL ( (194.65 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 7.5 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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