Name: Acetazolamide
Brand: Selleck Chemicals
SKU: S4506
Availability: InStock
Rating: 5 (8 reviews)

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Quality Control

Batch: Purity: 99.98%

99.98

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Carbonic Anhydrase Inhibitors	U-104 (SLC-0111) Acipimox Indisulam (E7070) Benzenesulfonamide Tioxolone 2-Aminobenzenesulfonamide

Solubility

In vitro
Batch:

DMSO : 44 mg/mL (197.97 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.200mg/ml (9.90mM)	Taking the 1 mL working solution as an example, add 50 μL of 44 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.730mg/ml (3.28mM)	Taking the 1 mL working solution as an example, add 50 μL of 14.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	222.25	Formula	C₄H₆N₄O₃S₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	59-66-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Diamox			SMILES	CC(=O)NC1=NN=C(S1)S(=O)(=O)N

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	Carbonic anhydrase 10 nM
In vitro	acetazolamide reduces invasiveness of cancer cells in vitro.
In vivo	Acetazolamide reduces inflammation-induced heat hyperalgesia, probably acting both peripherally and centrally. This compound shows significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation. It is clinically used to reduce intraocular pressure in glaucoma, to correct metabolic alkalosis, and to manage cerebral edema. Its treatment may also be beneficial as an adjunct to cancer chemotherapy, because it can produce additive tumor growth delays with anticancer drugs in vivo.
References	[1] https://pubmed.ncbi.nlm.nih.gov/15743922/ [2] https://pubmed.ncbi.nlm.nih.gov/15169637/ [3] https://pubmed.ncbi.nlm.nih.gov/10688890/ [4] http://accessmedicine.mhmedical.com/book.aspx?bookid=374

Applications

Methods	Biomarkers	Images	PMID
Growth inhibition assay	Cell viability		28235409

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-10-08)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00262470	ACTIVE_NOT_RECRUITING	Tachycardia; Chronic Orthostatic Intolerance	Satish R. Raj	1997-04	PHASE1; PHASE2
NCT06945848	RECRUITING	Idiopathic Intracranial Hypertension (IIH)	University Hospital, Montpellier	2026-01-05
NCT07737964	NOT_YET_RECRUITING	Heart Failure	University Hospital, Montpellier	2026-10	PHASE3
NCT05953610	RECRUITING	Obstructive Sleep Apnea	University of California, Los Angeles	2024-01-07	PHASE2
NCT06521476	RECRUITING	Central Sleep Apnea Comorbid With Opioid Use	Sanjay R Patel	2025-09-30	PHASE2
NCT07469722	NOT_YET_RECRUITING	Acute Heart Failure (AHF); Congestive Heart Failure Acute; Diuretic Effect; Lung Ultrasonography Score; Ultrasonography, Doppler; Point-of-care Ultrasound (POCUS)	Hospital de Clinicas de Porto Alegre	2026-03-23	PHASE2; PHASE3

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Handling Instructions

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Acetazolamide Carbonic Anhydrase inhibitor

Chemical Structure

Molecular Weight: 222.25