Home Metabolism Peroxidases inhibitor - Ferroptosis activator ML 210 For research use only.

ML 210

Synonyms: CID 49766530

ML-210 (CID 49766530) is a selective covalent inhibitor of cellular glutathione peroxidase 4 (GPX4) with EC50 of 0.04 μM and induces ferroptosis. ML-210 can selectively kill cells induced to express mutant RAS. ML-210 exhibits anti-cancer activity.

ML 210 Chemical Structure

ML 210 Chemical Structure

CAS No. 1360705-96-9

Purity & Quality Control

ML 210 Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
BJeLR Cytotoxicity assay 48 hrs Cytotoxicity against human BJeLR cells expressing HRAS G12V mutant after 48 hrs by alamar blue assay, IC50 = 0.071 μM. 22297109
BJ Cytotoxicity assay 48 hrs Cytotoxicity against human BJ cells expressing HRAS G12V mutant with alternative oncogenic constructs after 48 hrs by alamar blue assay, IC50 = 0.107 μM. 22297109
BJeH-LT Cytotoxicity assay 48 hrs Cytotoxicity against human BJeH-LT cells after 48 hrs by alamar blue assay, IC50 = 0.272 μM. 22297109
BJeH Cytotoxicity assay 48 hrs Cytotoxicity against human BJeH cells after 48 hrs by alamar blue assay, IC50 = 0.628 μM. 22297109
Click to View More Cell Line Experimental Data

Biological Activity

Description ML-210 (CID 49766530) is a selective covalent inhibitor of cellular glutathione peroxidase 4 (GPX4) with EC50 of 0.04 μM and induces ferroptosis. ML-210 can selectively kill cells induced to express mutant RAS. ML-210 exhibits anti-cancer activity.
Targets
RAS [1] GPX4 [2]
(Cell-free assay)
0.04 μM(EC50)
In vitro
In vitro

ML-210 has IC50s of 71 nM, 272 nM and 107nM for BJeLR (HRASV12), BJeH-LT (without HRASV12) and DRD cell lines, respectively[2].[1]

Chemical Information & Solubility

Molecular Weight 475.32 Formula

C22H20Cl2N4O4
CAS No. 1360705-96-9 SDF --
Smiles CC1=C(C(=NO1)C(=O)N2CCN(CC2)C(C3=CC=C(C=C3)Cl)C4=CC=C(C=C4)Cl)[N+](=O)[O-]
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 24 mg/mL ( (50.49 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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