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Mitomycin C

Antineoplastic and Immunosuppressive Antibiotics inhibitor

Catalog No.S8146 Purity:99.74%

research use only

Mitomycin C (Ametycine) is an antineoplastic antibiotic by inhibiting DNA synthesis, used to treat different cancers. Mitomycin C induces apoptosis in a caspases-dependent and Fas/CD95-independent manner.This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Mitomycin C Chemical Structure

Molecular Weight: 334.37

Purity & Quality Control

Batch: Purity: 99.74%

99.74

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Cell Culture and Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
human CH1 (ovarian) tumor cell	Cytotoxic assay		The compound was evaluated for the cytotoxicity against human CH1 (ovarian) tumor cell lines, IC50=40 nM	10698444
human HT-29 (colon) tumor cell	Cytotoxic assay		The compound was evaluated for the cytotoxicity against human HT-29 (colon) tumor cell lines, IC50=40 nM	10698444
human A2780 (ovarian) tumor cell	Cytotoxic assay		The compound was evaluated for the cytotoxicity against human A2780 (ovarian) tumor cell lines, IC50=50 nM	10698444
human colon cancer cell line HCT15	Cytotoxic assay	72 h	In vitro cytotoxic activity against human colon cancer cell line HCT15 after 72 hr of drug exposure determined by the SRB assay, IC50=70 nM	14980672
Human Caki-1 Renal CellCarcinoma Cell	Function assay	96 h	Inhibitory concentration against Human Caki-1 Renal CellCarcinoma Cell Lines (CD70+) at 96th hour, ic50=40 nM	15743178
SW480 colon cancer cell	Growth inhibition assay		In vitro inhibition of SW480 colon cancer cell line, IC50=7 nM	1920349
WiDr colon cancer cell	Growth inhibition assay		In vitro inhibition of WiDr colon cancer cell line, IC50=10 nM	1920349
HeLa	Function assay		In vitro anticellular activity against HeLa cells., IC50 = 0.81 μM.	1495011
L1210	Function assay		Inhibitory concentration on parentral (sensitive) L1210 leukemia cells., IC50 = 0.09 μM.	1906107
L1210	Function assay		Inhibitory concentration on multidrug-resistant L1210 leukemia cells., IC50 = 1.5 μM.	1906107
P388	Antitumor assay		Antitumor potency on P388 leukemia cells in culture, IC50 = 0.0508 μM.	1906109
HT-29	Antiproliferative assay	96 hrs	Antiproliferative activity against human HT-29 cells after 96 hrs by MTT assay, IC50 = 0.099 μM.	2778449
P388	Antiproliferative assay	48 hrs	Antiproliferative activity against mouse P388 cells after 48 hrs by MTT assay, IC50 = 3 μM.	2778449
AA8	Function assay	4 hrs	Inhibitory activity of compound for AA8 cells to reduce cell density by 50% (exposed to compound for 4 hours), IC50 = 0.00108 μM.	2909741
L1210	Growth inhibition assay	8-9 hrs	Inhibition of L1210 leukemia cells, after exposure to compound for period of 8-9 hr, ID50 = 0.18 μM.	3397995
L1210	Growth inhibition assay	1 hr	Inhibition of L1210 leukemia cells, after exposure to compound for period of 1 hr, ID50 = 3.1 μM.	3397995
EMT6	Function assay	1 hr	Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under hypoxic condition., ED50 = 0.001 μM.	7452674
EMT6	Function assay	1 hr	Compound is measured for surviving fractions of EMT6 tumor cells treated in culture with (0.50 uM) concentration for 1 hr under aerobic condition., ED50 = 0.7 μM.	7452674
V79	Function assay		Inhibitory concentration required to kill 50% of the cells was evaluated under anaerobic (N2) conditions from chinese hamster V79 cells., IC50 = 0.4 μM.	7966141
V79	Function assay		Inhibitory concentration required to kill 50% of the cells was evaluated under aerobic conditions from chinese hamster V79 cells., IC50 = 0.8 μM.	7966141
HeLa S3	Function assay		In vitro anticellular activity against HeLa S3 cells; 0.59-1.1, IC50 = 1.1 μM.	8021918
HeLa S3	Function assay		In vitro anticellular activity against HeLa S3 cells; 0.82-1.4, IC50 = 1.4 μM.	8021918
HeLa S3	Function assay		In vitro anticellular activity against HeLa S3 cells, IC50 = 0.59 μM.	8021919
P388	Function assay		Evaluation of inhibitory activity against drug sensitive P388 cells (P388/S) cells, IC50 = 0.048 μM.	8544183
P388	Function assay		Evaluation of inhibitory activity against multidrug resistant P388 cells (P388/ADR) cells, IC50 = 0.535 μM.	8544183
HL-60	Growth inhibition assay		Inhibition of cell growth was studied in human promyelocytic leukemic (HL-60) cells., IC50 = 0.05 μM.	8709111
DC-3F	Growth inhibition assay		Inhibition of cell growth was studied in chinese hamster lung cells (DC-3F), IC50 = 0.082 μM.	8709111
DC-3F/AD-II	Growth inhibition assay		Inhibition of cell growth was studied in chinese hamster lung cells resistant to actinomycin D (DC-3F/AD-II)., IC50 = 0.11 μM.	8709111
V79-379A	Function assay		In vitro concentration required to kill hypoxic cells V79-379A cells, C50 = 0.4 μM.	9240349
aerobic cell	Function assay		In vitro concentration required to kill 50% of the aerobic cells, C50 = 0.8 μM.	9240349
SupT1	Antiproliferative assay		Antiproliferative activity of compound was evaluated on SupT1 cells from human non-Hodgkin lymphoma, ID50 = 0.7 μM.	11012027
Molt3	Antiproliferative assay		Antiproliferative activity of compound was evaluated on Molt3 cells from human lymphoblastic leukemia, ID50 = 2.66 μM.	11012027
MCF-7	Antiproliferative assay		Antiproliferative activity of compound was evaluated on MCF-7 cells from human breast adenocarcinoma, ID50 = 42.92 μM.	11012027
lung cancer cell	Cytotoxicity assay		Cytotoxicity against human lung cancer cells, GI50 = 0.023 μM.	11473418
breast cancer cell	Cytotoxicity assay		Cytotoxicity against human breast cancer cells, GI50 = 0.034 μM.	11473418
CNS cancer cell	Cytotoxicity assay		Cytotoxicity against human CNS cancer cells, GI50 = 0.064 μM.	11473418
stomach cancer cell	Cytotoxicity assay		Cytotoxicity against human stomach cancer cells, GI50 = 0.1 μM.	11473418
ovarian cancer cell	Cytotoxicity assay		Cytotoxicity against human ovarian cancer cells, GI50 = 0.12 μM.	11473418
colon cancer cell	Cytotoxicity assay		Cytotoxicity against human colon cancer cells, GI50 = 0.18 μM.	11473418
HeLa	Cytotoxicity assay	5 to 8 days	Cytotoxicity against human HeLa cells assessed as inhibition of colony formation after 5 to 8 days by colony assay, IC50 = 0.068 μM.	11975488
K562	Function assay		In vitro inhibitory activity against K562 leukemia cells, IC50 = 0.001 μM.	12699386
K562	Function assay		In vitro inhibitory activity against K562 leukemia cells in the presence of pd2, IC50 = 0.0158 μM.	12699386
SCL9	Cytotoxicity assay		Cytotoxicity against human SCL9 cells by MTT assay, ED50 = 17.4 μM.	12880314
SCL37'6	Cytotoxicity assay		Cytotoxicity against human SCL37'6 cells by MTT assay, ED50 = 19 μM.	12880314
NUGC4	Cytotoxicity assay		Cytotoxicity against human NUGC4 cells by MTT assay, ED50 = 19.1 μM.	12880314
SCL	Cytotoxicity assay		Cytotoxicity against human SCL cells by MTT assay, ED50 = 20.2 μM.	12880314
SCL6	Cytotoxicity assay		Cytotoxicity against human SCL6 cells by MTT assay, ED50 = 20.7 μM.	12880314
KATO III	Cytotoxicity assay		Cytotoxicity against human KATO III cells by MTT assay, ED50 = 49 μM.	12880314
A549	Cytotoxicity assay	3 days	Cytotoxicity against human A549 cells after 3 days by SRB assay, IC50 = 0.3 μM.	16038545
KB	Cytotoxicity assay	3 days	Cytotoxicity against human KB cells after 3 days by SRB assay, IC50 = 0.6 μM.	16038545
HCT8	Cytotoxicity assay	3 days	Cytotoxicity against human HCT8 cells after 3 days by SRB assay, IC50 = 0.6 μM.	16038545
MCF7	Cytotoxicity assay	3 days	Cytotoxicity against human MCF7 cells after 3 days by SRB assay, IC50 = 1.5 μM.	16038545
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells assessed as cell viability by tryptan blue staining method, EC50 = 0.14 μM.	17950604
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells assessed as cell viability by tryptan blue staining method, EC50 = 0.43 μM.	17950604
Fb	Cytotoxicity assay		Cytotoxicity against human Fb cells assessed as cell viability by tryptan blue staining method, EC50 = 0.93 μM.	17950604
MCF7	Cytotoxicity assay		Cytotoxicity against human MCF7 cells assessed as cell viability by tryptan blue staining method, EC50 = 0.96 μM.	17950604
Hc	Cytotoxicity assay		Cytotoxicity against human Hc cells assessed as cell viability by tryptan blue staining method, EC50 = 1.46 μM.	17950604
IE	Cytotoxicity assay		Cytotoxicity against human IE cells assessed as cell viability by tryptan blue staining method, EC50 = 1.46 μM.	17950604
MPC5	Cytotoxicity assay		Cytotoxicity against human MPC5 cells assessed as cell viability by tryptan blue staining method, EC50 = 2.1 μM.	17950604
MCF7	Cytotoxicity assay		Cytotoxicity against human MCF7 cells by Alamar blue assay, IC50 = 0.2 μM.	18037301
A549	Cytotoxicity assay	72 hrs	Cytotoxicity against human A549 cells after 72 hrs, IC50 = 0.24 μM.	18037301
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells by Alamar blue assay, IC50 = 0.4 μM.	18037301
MCF7	Cytotoxicity assay	72 hrs	Cytotoxicity against human MCF7 cells after 72 hrs, IC50 = 1.2 μM.	18037301
A549	Cytotoxicity assay	24 hrs	Cytotoxicity against human A549 cells after 24 hrs, IC50 = 4.5 μM.	18037301
MCF7	Cytotoxicity assay	24 hrs	Cytotoxicity against human MCF7 cells after 24 hrs, IC50 = 5 μM.	18037301
HT29	Cytotoxicity assay	72 hrs	Cytotoxicity against human HT29 cells after 72 hrs by MTT assay, IC50 = 0.9 μM.	18222567
HT29	Cytotoxicity assay	72 hrs	Cytotoxicity against human HT29 cells after 72 hrs by MTT assay in presence of 2 uM dicoumarol, IC50 = 2.1 μM.	18222567
V-C8	Cytotoxicity assay	3 days	Cytotoxicity against BRCA2-deficient Chinese hamster V-C8 mutant cells after 3 days by WST1 cell viability assay, IC50 = 0.005 μM.	18505284
BAC29	Cytotoxicity assay	3 days	Cytotoxicity against BRCA2-proficient Chinese hamster BAC29 mutant cells after 3 days by WST1 cell viability assay, IC50 = 0.4 μM.	18505284
MCF7	Cytotoxicity assay	72 hrs	Cytotoxicity against human MCF7 cells after 72 hrs by Alamar Blue assay, IC50 = 0.2 μM.	18752870
A549	Cytotoxicity assay	72 hrs	Cytotoxicity against human A549 cells after 72 hrs by Alamar Blue assay, IC50 = 0.4 μM.	18752870
HCT8	Cytotoxicity assay	72 hrs	Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay, IC50 = 0.083 μM.	18845441
HCT8	Cytotoxicity assay	72 hrs	Cytotoxicity against human HCT8 cells after 72 hrs by SRB assay, IC90 = 5.7 μM.	18845441
WiDr	Cytotoxicity assay		Cytotoxicity against human WiDr cells by MTT assay, ED50 = 0.18 μM.	19061391
Daoy	Cytotoxicity assay		Cytotoxicity against human Daoy cells by MTT assay, ED50 = 0.21 μM.	19061391
HL60	Cytotoxicity assay	48 hrs	Cytotoxicity against human HL60 cells after 48 hrs by SRB assay, IC50 = 1.5 μM.	19432411
HeLa	Cytotoxicity assay	48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by SRB assay, IC50 = 3.3 μM.	19432411
SMMC7721	Cytotoxicity assay	48 hrs	Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay, IC50 = 5.4 μM.	19432411
PC3	Antiproliferative assay	72 hrs	Antiproliferative activity against human PC3 cells after 72 hrs by trypan blue exclusion assay, EC50 = 0.14 μM.	19674905
A549	Antiproliferative assay	72 hrs	Antiproliferative activity against human A549 cells after 72 hrs by trypan blue exclusion assay, EC50 = 0.43 μM.	19674905
Hs888Lu	Antiproliferative assay	72 hrs	Antiproliferative activity against human Hs888Lu cells after 72 hrs by trypan blue exclusion assay, EC50 = 0.56 μM.	19674905
MCF7	Antiproliferative assay	72 hrs	Antiproliferative activity against human MCF7 cells after 72 hrs by trypan blue exclusion assay, EC50 = 0.96 μM.	19674905
SVCT-M12	Antiproliferative assay	72 hrs	Antiproliferative activity against human SVCT-M12 cells after 72 hrs by trypan blue exclusion assay, EC50 = 0.96 μM.	19674905
IE	Antiproliferative assay	72 hrs	Antiproliferative activity against human IE cells after 72 hrs by trypan blue exclusion assay, EC50 = 1.46 μM.	19674905
MPC5	Antiproliferative assay	72 hrs	Antiproliferative activity against human MPC5 cells after 72 hrs by trypan blue exclusion assay, EC50 = 2.1 μM.	19674905
PC3	Growth inhibition assay	48 hrs	Growth inhibition of human PC3 cells after 48 hrs by sulforhodamine B assay, IC50 = 1.5 μM.	19939522
Hep2	Growth inhibition assay	48 hrs	Growth inhibition of human Hep2 cells after 48 hrs by sulforhodamine B assay, IC50 = 1.5 μM.	19939522
Daudi	Growth inhibition assay	24 to 72 hrs	Growth inhibition of human Daudi cells after 24 to 72 hrs by MTT assay, IC50 = 0.45 μM.	20117936
K562	Growth inhibition assay	24 to 72 hrs	Growth inhibition of human K562 cells after 24 to 72 hrs by MTT assay, IC50 = 0.47 μM.	20117936
K562-Lucena 1	Growth inhibition assay	24 to 72 hrs	Growth inhibition of human K562-Lucena 1 cells after 24 to 72 hrs by MTT assay, IC50 = 2.75 μM.	20117936
PBMC	Growth inhibition assay	24 to 72 hrs	Growth inhibition of phytohemagglutinin-activated human PBMC cells after 24 to 72 hrs by MTT assay, IC50 = 4.03 μM.	20117936
GM00637	Cytotoxicity assay	24 hrs	Cytotoxicity against human GM00637 cells after 24 hrs by MTT assay, IC50 = 0.9 μM.	20122765
DU145	Cytotoxicity assay	72 hrs	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay, IC50 = 0.17 μM.	20605274
HeLa	Cytotoxicity assay	72 hrs	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay, IC50 = 0.27 μM.	20605274
GM00637	Cytotoxicity assay	72 hrs	Cytotoxicity against human GM00637 cells after 72 hrs by MTT assay, IC50 = 0.46 μM.	20605274
DU145	Cytotoxicity assay	72 hrs	Cytotoxicity against human DU145 cells assessed as reduction in mean cell viability after 72 hrs by MTT assay, IC50 = 0.17 μM.	22522008
HeLa	Cytotoxicity assay	72 hrs	Cytotoxicity against human HeLa cells assessed as reduction in mean cell viability after 72 hrs by MTT assay, IC50 = 0.27 μM.	22522008
GM00637	Cytotoxicity assay	72 hrs	Cytotoxicity against human GM00637 cells assessed as reduction in mean cell viability after 72 hrs by MTT assay, IC50 = 0.46 μM.	22522008
HL60	Cytotoxicity assay	72 hrs	Cytotoxicity against human HL60 cells after 72 hrs by WST8 assay, IC50 = 0.1 μM.	22537363
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells by MTT assay, IC50 = 3 μM.	22545792
HL60	Cytotoxicity assay	48 hrs	Cytotoxicity against human HL60 cells after 48 hrs by SRB assay, IC50 = 1.8 μM.	22642381
HeLa	Cytotoxicity assay	48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by SRB assay, IC50 = 3.4 μM.	22642381
SMMC7721	Cytotoxicity assay	48 hrs	Cytotoxicity against human SMMC7721 cells after 48 hrs by SRB assay, IC50 = 5.6 μM.	22642381
A549	Cytotoxicity assay	72 hrs	Cytotoxicity against human A549 cells after 72 hrs by alamar blue assay, IC50 = 0.4 μM.	23123017
HCT116	Antiproliferative assay	48 hrs	Antiproliferative activity against human HCT116 cells after 48 hrs by sulforhodamine B assay, IC50 = 0.5 μM.	23501113
NCI-H23	Cytotoxicity assay	48 hrs	Cytotoxicity against human NCI-H23 cells after 48 hrs by MTT assay, IC50 = 2 μM.	23792352
SPCA1	Cytotoxicity assay	48 hrs	Cytotoxicity against human SPCA1 cells after 48 hrs by MTT assay, IC50 = 1.68 μM.	23999045
SGC7901	Cytotoxicity assay	48 hrs	Cytotoxicity against human SGC7901 cells after 48 hrs by MTT assay, IC50 = 2.05 μM.	23999045
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay, IC50 = 1.5 μM.	24056146
HeLa	Cytotoxicity assay	48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by SRB assay, IC50 = 2 μM.	24056146
MDA-MB-231	Cytotoxicity assay	5 days	Cytotoxicity against human MDA-MB-231 cells overexpressing DT-diaphorase after 5 days, EC50 = 0.103 μM.	24436995
MDA-MB-231	Cytotoxicity assay	5 days	Cytotoxicity against wild-type human MDA-MB-231 cells after 5 days, EC50 = 1.25 μM.	24436995
HCT15	Cytotoxicity assay	48 hrs	Cytotoxicity against human HCT15 cells after 48 hrs by sulforhodamine B assay, IC50 = 0.5 μM.	24910974
HeLa	Cytotoxicity assay	48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by SRB method, IC50 = 3 μM.	24913558
SMMC-7221	Cytotoxicity assay	48 hrs	Cytotoxicity against human SMMC-7221 cells after 48 hrs by SRB method, IC50 = 3 μM.	24913558
HL60	Cytotoxicity assay	48 hrs	Cytotoxicity against human HL60 cells after 48 hrs by SRB method, IC50 = 3 μM.	24913558
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells after 48 hrs by SRB assay, IC50 = 1.5 μM.	25176329
HeLa	Cytotoxicity assay	48 hrs	Cytotoxicity against human HeLa cells after 48 hrs by SRB assay, IC50 = 2 μM.	25176329
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells after 48 hrs by SRB assay, IC50 = 0.04 μM.	25259515
THP1	Cytotoxicity assay	48 hrs	Cytotoxicity against human THP1 cells after 48 hrs by SRB assay, IC50 = 0.1 μM.	25259515
MCF7	Cytotoxicity assay	48 hrs	Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay, IC50 = 0.2 μM.	25259515
A549	Cytotoxicity assay	24 hrs	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 24 hrs by WST8 assay, IC50 = 0.45 μM.	25442306
SF539	Cytotoxicity assay	72 hrs	Cytotoxicity against human SF539 cells after 72 hrs by MTS assay, IC50 = 0.038 μM.	26335269
M14	Cytotoxicity assay	72 hrs	Cytotoxicity against human M14 cells after 72 hrs by MTS assay, IC50 = 0.47 μM.	26335269
MDA-MB-468	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-468 cells after 72 hrs by MTS assay, IC50 = 0.5 μM.	26335269
SF295	Cytotoxicity assay	72 hrs	Cytotoxicity against human SF295 cells after 72 hrs by MTS assay, IC50 = 0.54 μM.	26335269
NCI-H226	Cytotoxicity assay	72 hrs	Cytotoxicity against human NCI-H226 cells after 72 hrs by MTS assay, IC50 = 0.58 μM.	26335269
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability at 10 to 100 uM after 48 hrs by SRB assay, EC50 = 5.2 μM.	26508550
HUT78	Antiproliferative assay	48 hrs	Antiproliferative activity against human HUT78 cells after 48 hrs by resazurin reduction assay, IC50 = 0.066 μM.	26541587
Jurkat T	Antiproliferative assay	48 hrs	Antiproliferative activity against human Jurkat T cells in hypoxic condition after 48 hrs by resazurin reduction assay, IC50 = 0.303 μM.	26541587
HL60	Antiproliferative assay	48 hrs	Antiproliferative activity against human HL60 cells after 48 hrs by resazurin reduction assay, IC50 = 0.41 μM.	26541587
Jurkat T	Antiproliferative assay	48 hrs	Antiproliferative activity against human Jurkat T cells after 48 hrs by resazurin reduction assay, IC50 = 0.578 μM.	26541587
HeLa	Antiproliferative assay	48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by resazurin reduction assay, IC50 = 1.87 μM.	26541587
HepG2	Antiproliferative assay	48 hrs	Antiproliferative activity against human HepG2 cells after 48 hrs by resazurin reduction assay, IC50 = 2.937 μM.	26541587
T47D	Antiproliferative assay	48 hrs	Antiproliferative activity against human T47D cells after 48 hrs by resazurin reduction assay, IC50 = 16.97 μM.	26541587
PC3	Cytotoxicity assay	72 hrs	Cytotoxicity against human PC3 cells after 72 hrs by CCK-8 assay, IC50 = 1.4 μM.	26615890
A549	Cytotoxicity assay	72 hrs	Cytotoxicity against human A549 cells after 72 hrs by CCK-8 assay, IC50 = 1.7 μM.	26615890
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.5 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.5 μM.	27089214
HT-29	Antiproliferative assay	72 hrs	Antiproliferative activity against human HT-29 cells expressing NQO1 after 72 hrs by MTT assay, IC50 = 7.099 μM.	28395199
NCI-H596	Antiproliferative assay	72 hrs	Antiproliferative activity against NQO1 deficient human NCI-H596 cells after 72 hrs by MTT assay, IC50 = 11.969 μM.	28395199
A549	Antiproliferative assay	72 hrs	Antiproliferative activity against human A549 cells expressing NQO1 after 72 hrs by MTT assay, IC50 = 15.525 μM.	28395199
LO2	Cytotoxicity assay	72 hrs	Cytotoxicity against human LO2 cells after 72 hrs by MTT assay, IC50 = 25.023 μM.	28395199
CHOK1	Cytotoxicity assay	24 hrs	Cytotoxicity against CHOK1 cells incubated for 24 hrs by MTT asasy, IC50 = 13.2 μM.	28615134
A549	Growth inhibition assay	72 hrs	Growth inhibition of human A549 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 0.08 μM.	28682609
SKOV3	Growth inhibition assay	72 hrs	Growth inhibition of human SKOV3 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 0.1 μM.	28682609
HCT116	Growth inhibition assay	72 hrs	Growth inhibition of human HCT116 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 0.2 μM.	28682609
786-O	Growth inhibition assay	72 hrs	Growth inhibition of human 786-O cells after 72 hrs by Cell-Titer Glo assay, GI50 = 0.21 μM.	28682609
BxPC3	Growth inhibition assay	72 hrs	Growth inhibition of human BxPC3 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 0.38 μM.	28682609
HEC6	Growth inhibition assay	72 hrs	Growth inhibition of human HEC6 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 1.4 μM.	28682609
MKN74	Growth inhibition assay	72 hrs	Growth inhibition of human MKN74 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 1.7 μM.	28682609
U87MG	Growth inhibition assay	72 hrs	Growth inhibition of human U87MG cells after 72 hrs by Cell-Titer Glo assay, GI50 = 1.8 μM.	28682609
HuH7	Growth inhibition assay	72 hrs	Growth inhibition of human HuH7 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 2 μM.	28682609
MCF7	Growth inhibition assay	72 hrs	Growth inhibition of human MCF7 cells after 72 hrs by Cell-Titer Glo assay, GI50 = 2.5 μM.	28682609
MDA-MB-231	Antiproliferative assay	48 hrs	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 4.4 μM.	29280632
HL60	Antiproliferative assay	48 hrs	Antiproliferative activity against human HL60 cells after 48 hrs by CCK8 assay, IC50 = 0.19 μM.	29475587
HeLa	Antiproliferative assay	48 hrs	Antiproliferative activity against human HeLa cells after 48 hrs by CCK8 assay, IC50 = 1.13 μM.	29475587
NTERA-S-cl-D1	Growth inhibition assay		IC50 = 0.0221 nM	SANGER
MOLT-4	Growth inhibition assay		IC50 = 0.0325 nM	SANGER
J82	Growth inhibition assay		IC50 = 0.119 nM	SANGER
697	Growth inhibition assay		IC50 = 0.1283 nM	SANGER
KYSE-510	Growth inhibition assay		IC50 = 0.1486 nM	SANGER
NCI-H2170	Growth inhibition assay		IC50 = 0.1513 nM	SANGER
BE-13	Growth inhibition assay		IC50 = 0.1673 nM	SANGER
LC-2-ad	Growth inhibition assay		IC50 = 0.2492 nM	SANGER
IMR-5	Growth inhibition assay		IC50 = 0.3076 nM	SANGER
EW-16	Growth inhibition assay		IC50 = 0.31 nM	SANGER
MC-IXC	Growth inhibition assay		IC50 = 0.638 nM	SANGER
SW872	Growth inhibition assay		IC50 = 0.676 nM	SANGER
NCI-H2228	Growth inhibition assay		IC50 = 0.7963 nM	SANGER
NALM-6	Growth inhibition assay		IC50 = 1.066 nM	SANGER
ES4	Growth inhibition assay		IC50 = 1.555 nM	SANGER
KE-37	Growth inhibition assay		IC50 = 1.636 nM	SANGER
IST-MEL1	Growth inhibition assay		IC50 = 1.684 nM	SANGER
ES8	Growth inhibition assay		IC50 = 1.712 nM	SANGER
MV-4-11	Growth inhibition assay		IC50 = 1.732 nM	SANGER
639-V	Growth inhibition assay		IC50 = 1.759 nM	SANGER
OCI-AML2	Growth inhibition assay		IC50 = 1.886 nM	SANGER
SK-LU-1	Growth inhibition assay		IC50 = 2.217 nM	SANGER
REH	Growth inhibition assay		IC50 = 2.548 nM	SANGER
PSN1	Growth inhibition assay		IC50 = 2.554 nM	SANGER
SBC-5	Growth inhibition assay		IC50 = 2.589 nM	SANGER
HT-3	Growth inhibition assay		IC50 = 2.928 nM	SANGER
SIG-M5	Growth inhibition assay		IC50 = 3.524 nM	SANGER
ES7	Growth inhibition assay		IC50 = 3.653 nM	SANGER
CHP-212	Growth inhibition assay		IC50 = 4.368 nM	SANGER
P12-ICHIKAWA	Growth inhibition assay		IC50 = 4.787 nM	SANGER
HUTU-80	Growth inhibition assay		IC50 = 4.83 nM	SANGER
P30-OHK	Growth inhibition assay		IC50 = 4.842 nM	SANGER
NCI-H1651	Growth inhibition assay		IC50 = 4.99 nM	SANGER
COR-L279	Growth inhibition assay		IC50 = 5.545 nM	SANGER
CMK	Growth inhibition assay		IC50 = 5.827 nM	SANGER
MHH-CALL-2	Growth inhibition assay		IC50 = 6.06 nM	SANGER
BV-173	Growth inhibition assay		IC50 = 6.067 nM	SANGER
NCI-H209	Growth inhibition assay		IC50 = 6.168 nM	SANGER
NCI-H460	Growth inhibition assay		IC50 = 6.452 nM	SANGER
DU-145	Growth inhibition assay		IC50 = 6.519 nM	SANGER
769-P	Growth inhibition assay		IC50 = 7.112 nM	SANGER
ES1	Growth inhibition assay		IC50 = 7.288 nM	SANGER
COLO-668	Growth inhibition assay		IC50 = 7.545 nM	SANGER
NKM-1	Growth inhibition assay		IC50 = 7.831 nM	SANGER
ES3	Growth inhibition assay		IC50 = 7.882 nM	SANGER
SCC-3	Growth inhibition assay		IC50 = 8.519 nM	SANGER
SU-DHL-1	Growth inhibition assay		IC50 = 8.609 nM	SANGER
ML-2	Growth inhibition assay		IC50 = 8.781 nM	SANGER
SF295	Growth inhibition assay		IC50 = 11.48 nM	SANGER
EW-7	Growth inhibition assay		IC50 = 12.14 nM	SANGER
H4	Growth inhibition assay		IC50 = 12.44 nM	SANGER
LB2241-RCC	Growth inhibition assay		IC50 = 13.19 nM	SANGER
SR	Growth inhibition assay		IC50 = 13.46 nM	SANGER
LB1047-RCC	Growth inhibition assay		IC50 = 13.6 nM	SANGER
MCF7	Growth inhibition assay		IC50 = 13.9 nM	SANGER
ESS-1	Growth inhibition assay		IC50 = 13.92 nM	SANGER
NCI-H2122	Growth inhibition assay		IC50 = 13.96 nM	SANGER
786-0	Growth inhibition assay		IC50 = 14.01 nM	SANGER
COLO-205	Growth inhibition assay		IC50 = 14.33 nM	SANGER
HAL-01	Growth inhibition assay		IC50 = 15.73 nM	SANGER
MHH-ES-1	Growth inhibition assay		IC50 = 16.26 nM	SANGER
NUGC-3	Growth inhibition assay		IC50 = 16.95 nM	SANGER
RPMI-8402	Growth inhibition assay		IC50 = 17.13 nM	SANGER
ATN-1	Growth inhibition assay		IC50 = 17.39 nM	SANGER
NCI-H441	Growth inhibition assay		IC50 = 17.6 nM	SANGER
LB996-RCC	Growth inhibition assay		IC50 = 17.68 nM	SANGER
CAL-148	Growth inhibition assay		IC50 = 17.83 nM	SANGER
CTV-1	Growth inhibition assay		IC50 = 18.67 nM	SANGER
HOP-62	Growth inhibition assay		IC50 = 20.2 nM	SANGER
CAKI-1	Growth inhibition assay		IC50 = 23.32 nM	SANGER
PA-1	Growth inhibition assay		IC50 = 23.51 nM	SANGER
HT-144	Growth inhibition assay		IC50 = 23.75 nM	SANGER
LOUCY	Growth inhibition assay		IC50 = 26.19 nM	SANGER
BPH-1	Growth inhibition assay		IC50 = 29.9 nM	SANGER
LS-1034	Growth inhibition assay		IC50 = 30.16 nM	SANGER
23132-87	Growth inhibition assay		IC50 = 30.53 nM	SANGER
BB30-HNC	Growth inhibition assay		IC50 = 30.61 nM	SANGER
CAL-51	Growth inhibition assay		IC50 = 30.86 nM	SANGER
ALL-PO	Growth inhibition assay		IC50 = 30.98 nM	SANGER
5637	Growth inhibition assay		IC50 = 31.93 nM	SANGER
KYSE-150	Growth inhibition assay		IC50 = 34.09 nM	SANGER
RS4-11	Growth inhibition assay		IC50 = 34.86 nM	SANGER
A3-KAW	Growth inhibition assay		IC50 = 34.96 nM	SANGER
NCI-H719	Growth inhibition assay		IC50 = 35.12 nM	SANGER
GI-1	Growth inhibition assay		IC50 = 35.16 nM	SANGER
HEL	Growth inhibition assay		IC50 = 35.8 nM	SANGER
TE-8	Growth inhibition assay		IC50 = 35.86 nM	SANGER
HT-29	Growth inhibition assay		IC50 = 36.02 nM	SANGER
SW1783	Growth inhibition assay		IC50 = 38.58 nM	SANGER
CGTH-W-1	Growth inhibition assay		IC50 = 38.74 nM	SANGER
MEL-HO	Growth inhibition assay		IC50 = 39.4 nM	SANGER
SW620	Growth inhibition assay		IC50 = 39.79 nM	SANGER
ME-180	Growth inhibition assay		IC50 = 40.11 nM	SANGER
CAL-27	Growth inhibition assay		IC50 = 41.18 nM	SANGER
A431	Growth inhibition assay		IC50 = 43.77 nM	SANGER
SW954	Growth inhibition assay		IC50 = 44.91 nM	SANGER
CAL-39	Growth inhibition assay		IC50 = 46.49 nM	SANGER
LU-134-A	Growth inhibition assay		IC50 = 46.72 nM	SANGER
DU-4475	Growth inhibition assay		IC50 = 47.47 nM	SANGER
DEL	Growth inhibition assay		IC50 = 48.39 nM	SANGER
HGC-27	Growth inhibition assay		IC50 = 49.29 nM	SANGER
RH-1	Growth inhibition assay		IC50 = 49.57 nM	SANGER
SK-MES-1	Growth inhibition assay		IC50 = 51.12 nM	SANGER
MEL-JUSO	Growth inhibition assay		IC50 = 51.25 nM	SANGER
VA-ES-BJ	Growth inhibition assay		IC50 = 52.16 nM	SANGER
EW-13	Growth inhibition assay		IC50 = 52.51 nM	SANGER
Ca-Ski	Growth inhibition assay		IC50 = 53.75 nM	SANGER
LXF-289	Growth inhibition assay		IC50 = 55.37 nM	SANGER
IM-9	Growth inhibition assay		IC50 = 56.22 nM	SANGER
HCE-4	Growth inhibition assay		IC50 = 57.94 nM	SANGER
TYK-nu	Growth inhibition assay		IC50 = 58.73 nM	SANGER
NCI-H748	Growth inhibition assay		IC50 = 59.54 nM	SANGER
ABC-1	Growth inhibition assay		IC50 = 61.2 nM	SANGER
CCRF-CEM	Growth inhibition assay		IC50 = 61.68 nM	SANGER
EW-3	Growth inhibition assay		IC50 = 61.95 nM	SANGER
NCI-H2342	Growth inhibition assay		IC50 = 62.51 nM	SANGER
SK-PN-DW	Growth inhibition assay		IC50 = 63.76 nM	SANGER
EB2	Growth inhibition assay		IC50 = 65.36 nM	SANGER
CAL-85-1	Growth inhibition assay		IC50 = 65.88 nM	SANGER
NCI-H526	Growth inhibition assay		IC50 = 67.37 nM	SANGER
MFH-ino	Growth inhibition assay		IC50 = 70.61 nM	SANGER
ACHN	Growth inhibition assay		IC50 = 72.08 nM	SANGER
MONO-MAC-6	Growth inhibition assay		IC50 = 72.35 nM	SANGER
CHP-126	Growth inhibition assay		IC50 = 72.44 nM	SANGER
SW1710	Growth inhibition assay		IC50 = 72.58 nM	SANGER
TE-10	Growth inhibition assay		IC50 = 72.6 nM	SANGER
TE-15	Growth inhibition assay		IC50 = 73.7 nM	SANGER
PANC-10-05	Growth inhibition assay		IC50 = 73.75 nM	SANGER
BHY	Growth inhibition assay		IC50 = 75.28 nM	SANGER
HSC-3	Growth inhibition assay		IC50 = 75.54 nM	SANGER
COLO-800	Growth inhibition assay		IC50 = 78.66 nM	SANGER
NB10	Growth inhibition assay		IC50 = 81.65 nM	SANGER
SK-UT-1	Growth inhibition assay		IC50 = 82.8 nM	SANGER
HSC-2	Growth inhibition assay		IC50 = 83.42 nM	SANGER
GP5d	Growth inhibition assay		IC50 = 84 nM	SANGER
A204	Growth inhibition assay		IC50 = 84.19 nM	SANGER
BFTC-909	Growth inhibition assay		IC50 = 85.27 nM	SANGER
NCI-H2087	Growth inhibition assay		IC50 = 85.69 nM	SANGER
G-402	Growth inhibition assay		IC50 = 85.92 nM	SANGER
647-V	Growth inhibition assay		IC50 = 87.39 nM	SANGER
Daoy	Growth inhibition assay		IC50 = 88.01 nM	SANGER
EW-1	Growth inhibition assay		IC50 = 88.07 nM	SANGER
BC-1	Growth inhibition assay		IC50 = 88.81 nM	SANGER
OAW-42	Growth inhibition assay		IC50 = 89.42 nM	SANGER
COLO-679	Growth inhibition assay		IC50 = 90.25 nM	SANGER
TE-11	Growth inhibition assay		IC50 = 91.77 nM	SANGER
BFTC-905	Growth inhibition assay		IC50 = 92.69 nM	SANGER
KP-4	Growth inhibition assay		IC50 = 93.15 nM	SANGER
DOHH-2	Growth inhibition assay		IC50 = 93.83 nM	SANGER
L-540	Growth inhibition assay		IC50 = 95.14 nM	SANGER
DSH1	Growth inhibition assay		IC50 = 96.53 nM	SANGER
M059J	Growth inhibition assay		IC50 = 100.6 nM	SANGER
UM-UC-3	Growth inhibition assay		IC50 = 101.83 nM	SANGER
ES5	Growth inhibition assay		IC50 = 102.42 nM	SANGER
KYSE-270	Growth inhibition assay		IC50 = 102.84 nM	SANGER
OCUB-M	Growth inhibition assay		IC50 = 102.98 nM	SANGER
C-4-II	Growth inhibition assay		IC50 = 103.51 nM	SANGER
BOKU	Growth inhibition assay		IC50 = 103.78 nM	SANGER
8-MG-BA	Growth inhibition assay		IC50 = 104 nM	SANGER
SW982	Growth inhibition assay		IC50 = 104.73 nM	SANGER
AGS	Growth inhibition assay		IC50 = 105.8 nM	SANGER
IGROV-1	Growth inhibition assay		IC50 = 105.97 nM	SANGER
HL-60	Growth inhibition assay		IC50 = 106.06 nM	SANGER
A2780	Growth inhibition assay		IC50 = 106.63 nM	SANGER
HOS	Growth inhibition assay		IC50 = 106.7 nM	SANGER
HuP-T4	Growth inhibition assay		IC50 = 108.1 nM	SANGER
ETK-1	Growth inhibition assay		IC50 = 108.24 nM	SANGER
IA-LM	Growth inhibition assay		IC50 = 108.63 nM	SANGER
MRK-nu-1	Growth inhibition assay		IC50 = 110.25 nM	SANGER
SUP-T1	Growth inhibition assay		IC50 = 110.4 nM	SANGER
A2058	Growth inhibition assay		IC50 = 115.1 nM	SANGER
CAPAN-1	Growth inhibition assay		IC50 = 116.4 nM	SANGER
NCI-H1355	Growth inhibition assay		IC50 = 116.8 nM	SANGER
NCI-H1618	Growth inhibition assay		IC50 = 117.76 nM	SANGER
QIMR-WIL	Growth inhibition assay		IC50 = 119.58 nM	SANGER
HCC1599	Growth inhibition assay		IC50 = 120.17 nM	SANGER
GR-ST	Growth inhibition assay		IC50 = 121.34 nM	SANGER
G-361	Growth inhibition assay		IC50 = 123.93 nM	SANGER
RKO	Growth inhibition assay		IC50 = 125.29 nM	SANGER
NCI-H1437	Growth inhibition assay		IC50 = 126.26 nM	SANGER
SF539	Growth inhibition assay		IC50 = 127.02 nM	SANGER
ACN	Growth inhibition assay		IC50 = 127.91 nM	SANGER
FADU	Growth inhibition assay		IC50 = 128.29 nM	SANGER
U251	Growth inhibition assay		IC50 = 128.73 nM	SANGER
KALS-1	Growth inhibition assay		IC50 = 129.14 nM	SANGER
KM-H2	Growth inhibition assay		IC50 = 130.45 nM	SANGER
SJSA-1	Growth inhibition assay		IC50 = 131.56 nM	SANGER
TE-5	Growth inhibition assay		IC50 = 132.24 nM	SANGER
SNG-M	Growth inhibition assay		IC50 = 134.17 nM	SANGER
HCC1806	Growth inhibition assay		IC50 = 134.54 nM	SANGER
NCI-H1734	Growth inhibition assay		IC50 = 136.85 nM	SANGER
LCLC-97TM1	Growth inhibition assay		IC50 = 138.72 nM	SANGER
NH-12	Growth inhibition assay		IC50 = 139.13 nM	SANGER
A375	Growth inhibition assay		IC50 = 139.42 nM	SANGER
NCI-SNU-5	Growth inhibition assay		IC50 = 141.21 nM	SANGER
SK-OV-3	Growth inhibition assay		IC50 = 142.64 nM	SANGER
22RV1	Growth inhibition assay		IC50 = 144.07 nM	SANGER
SW1573	Growth inhibition assay		IC50 = 144.68 nM	SANGER
BB65-RCC	Growth inhibition assay		IC50 = 144.71 nM	SANGER
MES-SA	Growth inhibition assay		IC50 = 144.94 nM	SANGER
K052	Growth inhibition assay		IC50 = 149.34 nM	SANGER
KU812	Growth inhibition assay		IC50 = 149.96 nM	SANGER
NOMO-1	Growth inhibition assay		IC50 = 151.41 nM	SANGER
Ca9-22	Growth inhibition assay		IC50 = 155.57 nM	SANGER
8305C	Growth inhibition assay		IC50 = 155.75 nM	SANGER
NCI-SNU-1	Growth inhibition assay		IC50 = 156.24 nM	SANGER
HC-1	Growth inhibition assay		IC50 = 156.51 nM	SANGER
SW962	Growth inhibition assay		IC50 = 157.55 nM	SANGER
HCC2998	Growth inhibition assay		IC50 = 160.13 nM	SANGER
DMS-273	Growth inhibition assay		IC50 = 160.83 nM	SANGER
MC-CAR	Growth inhibition assay		IC50 = 160.91 nM	SANGER
Caov-3	Growth inhibition assay		IC50 = 161.72 nM	SANGER
EoL-1-cell	Growth inhibition assay		IC50 = 162.39 nM	SANGER
MKN45	Growth inhibition assay		IC50 = 165.04 nM	SANGER
TE-1	Growth inhibition assay		IC50 = 165.38 nM	SANGER
HMV-II	Growth inhibition assay		IC50 = 166.06 nM	SANGER
SK-NEP-1	Growth inhibition assay		IC50 = 166.53 nM	SANGER
PANC-03-27	Growth inhibition assay		IC50 = 167.16 nM	SANGER
RPMI-7951	Growth inhibition assay		IC50 = 167.55 nM	SANGER
NCI-H520	Growth inhibition assay		IC50 = 168.65 nM	SANGER
NCI-H522	Growth inhibition assay		IC50 = 169.45 nM	SANGER
Detroit562	Growth inhibition assay		IC50 = 170.26 nM	SANGER
SF268	Growth inhibition assay		IC50 = 170.86 nM	SANGER
T84	Growth inhibition assay		IC50 = 173.34 nM	SANGER
NB69	Growth inhibition assay		IC50 = 175.06 nM	SANGER
OVCAR-8	Growth inhibition assay		IC50 = 175.54 nM	SANGER
RT-112	Growth inhibition assay		IC50 = 177.98 nM	SANGER
DoTc2-4510	Growth inhibition assay		IC50 = 180.37 nM	SANGER
J-RT3-T3-5	Growth inhibition assay		IC50 = 185.8 nM	SANGER
NB1	Growth inhibition assay		IC50 = 186.61 nM	SANGER
NEC8	Growth inhibition assay		IC50 = 186.77 nM	SANGER
TUR	Growth inhibition assay		IC50 = 189.67 nM	SANGER
TE-12	Growth inhibition assay		IC50 = 189.83 nM	SANGER
WSU-NHL	Growth inhibition assay		IC50 = 192.04 nM	SANGER
MHH-NB-11	Growth inhibition assay		IC50 = 192.55 nM	SANGER
SW48	Growth inhibition assay		IC50 = 193.36 nM	SANGER
C3A	Growth inhibition assay		IC50 = 194.31 nM	SANGER
RPMI-2650	Growth inhibition assay		IC50 = 195.75 nM	SANGER
VMRC-RCZ	Growth inhibition assay		IC50 = 198.77 nM	SANGER
SK-CO-1	Growth inhibition assay		IC50 = 0.20181 μM	SANGER
KYSE-180	Growth inhibition assay		IC50 = 0.20219 μM	SANGER
KGN	Growth inhibition assay		IC50 = 0.20713 μM	SANGER
A388	Growth inhibition assay		IC50 = 0.20722 μM	SANGER
NCI-H2030	Growth inhibition assay		IC50 = 0.21132 μM	SANGER
NCI-H727	Growth inhibition assay		IC50 = 0.2118 μM	SANGER
BC-3	Growth inhibition assay		IC50 = 0.21208 μM	SANGER
CCF-STTG1	Growth inhibition assay		IC50 = 0.21559 μM	SANGER
NMC-G1	Growth inhibition assay		IC50 = 0.21675 μM	SANGER
NCI-H1299	Growth inhibition assay		IC50 = 0.21841 μM	SANGER
RPMI-6666	Growth inhibition assay		IC50 = 0.21961 μM	SANGER
D-283MED	Growth inhibition assay		IC50 = 0.22069 μM	SANGER
AM-38	Growth inhibition assay		IC50 = 0.22116 μM	SANGER
LU-99A	Growth inhibition assay		IC50 = 0.22424 μM	SANGER
KYSE-70	Growth inhibition assay		IC50 = 0.22431 μM	SANGER
CHL-1	Growth inhibition assay		IC50 = 0.22516 μM	SANGER
CESS	Growth inhibition assay		IC50 = 0.2254 μM	SANGER
A4-Fuk	Growth inhibition assay		IC50 = 0.22543 μM	SANGER
MG-63	Growth inhibition assay		IC50 = 0.22618 μM	SANGER
MKN28	Growth inhibition assay		IC50 = 0.23009 μM	SANGER
NCI-H1417	Growth inhibition assay		IC50 = 0.2369 μM	SANGER
BCPAP	Growth inhibition assay		IC50 = 0.2396 μM	SANGER
COR-L23	Growth inhibition assay		IC50 = 0.24094 μM	SANGER
SIMA	Growth inhibition assay		IC50 = 0.24142 μM	SANGER
CAL-62	Growth inhibition assay		IC50 = 0.24185 μM	SANGER
ARH-77	Growth inhibition assay		IC50 = 0.24331 μM	SANGER
COLO-680N	Growth inhibition assay		IC50 = 0.2441 μM	SANGER
T-24	Growth inhibition assay		IC50 = 0.24577 μM	SANGER
PF-382	Growth inhibition assay		IC50 = 0.25063 μM	SANGER
NCI-H810	Growth inhibition assay		IC50 = 0.25161 μM	SANGER
NCI-H1882	Growth inhibition assay		IC50 = 0.25709 μM	SANGER
A101D	Growth inhibition assay		IC50 = 0.25745 μM	SANGER
NCI-H2126	Growth inhibition assay		IC50 = 0.2602 μM	SANGER
VM-CUB-1	Growth inhibition assay		IC50 = 0.26082 μM	SANGER
OS-RC-2	Growth inhibition assay		IC50 = 0.2637 μM	SANGER
MIA-PaCa-2	Growth inhibition assay		IC50 = 0.27545 μM	SANGER
SW837	Growth inhibition assay		IC50 = 0.27613 μM	SANGER
NCI-H358	Growth inhibition assay		IC50 = 0.27626 μM	SANGER
NCI-H1703	Growth inhibition assay		IC50 = 0.27688 μM	SANGER
NCI-H1650	Growth inhibition assay		IC50 = 0.28257 μM	SANGER
SW626	Growth inhibition assay		IC50 = 0.28622 μM	SANGER
MFE-296	Growth inhibition assay		IC50 = 0.28623 μM	SANGER
NCI-H2009	Growth inhibition assay		IC50 = 0.28857 μM	SANGER
NCI-H2405	Growth inhibition assay		IC50 = 0.29434 μM	SANGER
BL-41	Growth inhibition assay		IC50 = 0.2949 μM	SANGER
SNU-C2B	Growth inhibition assay		IC50 = 0.29505 μM	SANGER
no-10	Growth inhibition assay		IC50 = 0.2996 μM	SANGER
A427	Growth inhibition assay		IC50 = 0.30133 μM	SANGER
SNB75	Growth inhibition assay		IC50 = 0.30346 μM	SANGER
CTB-1	Growth inhibition assay		IC50 = 0.30637 μM	SANGER
BT-20	Growth inhibition assay		IC50 = 0.31197 μM	SANGER
GT3TKB	Growth inhibition assay		IC50 = 0.31823 μM	SANGER
COR-L88	Growth inhibition assay		IC50 = 0.31914 μM	SANGER
Calu-6	Growth inhibition assay		IC50 = 0.31971 μM	SANGER
Calu-3	Growth inhibition assay		IC50 = 0.32095 μM	SANGER
IGR-1	Growth inhibition assay		IC50 = 0.3216 μM	SANGER
LS-411N	Growth inhibition assay		IC50 = 0.32176 μM	SANGER
LAN-6	Growth inhibition assay		IC50 = 0.32779 μM	SANGER
ONS-76	Growth inhibition assay		IC50 = 0.32956 μM	SANGER
MZ7-mel	Growth inhibition assay		IC50 = 0.33256 μM	SANGER
LB647-SCLC	Growth inhibition assay		IC50 = 0.33608 μM	SANGER
OE33	Growth inhibition assay		IC50 = 0.3378 μM	SANGER
NCI-H1770	Growth inhibition assay		IC50 = 0.34265 μM	SANGER
MS-1	Growth inhibition assay		IC50 = 0.34402 μM	SANGER
NCI-H650	Growth inhibition assay		IC50 = 0.34407 μM	SANGER
MDA-MB-468	Growth inhibition assay		IC50 = 0.34531 μM	SANGER
A253	Growth inhibition assay		IC50 = 0.34564 μM	SANGER
LOXIMVI	Growth inhibition assay		IC50 = 0.3525 μM	SANGER
BB49-HNC	Growth inhibition assay		IC50 = 0.35252 μM	SANGER
HT-1080	Growth inhibition assay		IC50 = 0.35371 μM	SANGER
NB5	Growth inhibition assay		IC50 = 0.3579 μM	SANGER
LK-2	Growth inhibition assay		IC50 = 0.35814 μM	SANGER
KS-1	Growth inhibition assay		IC50 = 0.36415 μM	SANGER
OE19	Growth inhibition assay		IC50 = 0.36496 μM	SANGER
U031	Growth inhibition assay		IC50 = 0.3658 μM	SANGER
HCE-T	Growth inhibition assay		IC50 = 0.37153 μM	SANGER
NCI-H1304	Growth inhibition assay		IC50 = 0.37523 μM	SANGER
SKG-IIIa	Growth inhibition assay		IC50 = 0.37738 μM	SANGER
GDM-1	Growth inhibition assay		IC50 = 0.37746 μM	SANGER
CPC-N	Growth inhibition assay		IC50 = 0.38066 μM	SANGER
KYSE-140	Growth inhibition assay		IC50 = 0.38134 μM	SANGER
SH-4	Growth inhibition assay		IC50 = 0.38726 μM	SANGER
IST-SL2	Growth inhibition assay		IC50 = 0.38762 μM	SANGER
SCC-4	Growth inhibition assay		IC50 = 0.3877 μM	SANGER
OMC-1	Growth inhibition assay		IC50 = 0.38955 μM	SANGER
Daudi	Growth inhibition assay		IC50 = 0.39242 μM	SANGER
JVM-2	Growth inhibition assay		IC50 = 0.39734 μM	SANGER
NCI-H510A	Growth inhibition assay		IC50 = 0.40282 μM	SANGER
NCI-H1838	Growth inhibition assay		IC50 = 0.40419 μM	SANGER
GCIY	Growth inhibition assay		IC50 = 0.41099 μM	SANGER
NCI-H747	Growth inhibition assay		IC50 = 0.41159 μM	SANGER
NCI-H1694	Growth inhibition assay		IC50 = 0.41528 μM	SANGER
UACC-893	Growth inhibition assay		IC50 = 0.41801 μM	SANGER
DMS-153	Growth inhibition assay		IC50 = 0.42526 μM	SANGER
GAMG	Growth inhibition assay		IC50 = 0.42821 μM	SANGER
LB373-MEL-D	Growth inhibition assay		IC50 = 0.42962 μM	SANGER
8505C	Growth inhibition assay		IC50 = 0.42983 μM	SANGER
DB	Growth inhibition assay		IC50 = 0.42995 μM	SANGER
LoVo	Growth inhibition assay		IC50 = 0.43068 μM	SANGER
MZ2-MEL	Growth inhibition assay		IC50 = 0.43247 μM	SANGER
L-363	Growth inhibition assay		IC50 = 0.43417 μM	SANGER
HSC-4	Growth inhibition assay		IC50 = 0.43445 μM	SANGER
NCI-N87	Growth inhibition assay		IC50 = 0.43812 μM	SANGER
SK-LMS-1	Growth inhibition assay		IC50 = 0.43929 μM	SANGER
HO-1-N-1	Growth inhibition assay		IC50 = 0.44064 μM	SANGER
OAW-28	Growth inhibition assay		IC50 = 0.44065 μM	SANGER
SBC-1	Growth inhibition assay		IC50 = 0.4477 μM	SANGER
LNCaP-Clone-FGC	Growth inhibition assay		IC50 = 0.45008 μM	SANGER
MZ1-PC	Growth inhibition assay		IC50 = 0.45011 μM	SANGER
GB-1	Growth inhibition assay		IC50 = 0.45218 μM	SANGER
LCLC-103H	Growth inhibition assay		IC50 = 0.46087 μM	SANGER
YT	Growth inhibition assay		IC50 = 0.46333 μM	SANGER
HCT-15	Growth inhibition assay		IC50 = 0.46462 μM	SANGER
CaR-1	Growth inhibition assay		IC50 = 0.46878 μM	SANGER
SK-MEL-30	Growth inhibition assay		IC50 = 0.47431 μM	SANGER
TCCSUP	Growth inhibition assay		IC50 = 0.4817 μM	SANGER
C-33-A	Growth inhibition assay		IC50 = 0.48687 μM	SANGER
OVCAR-5	Growth inhibition assay		IC50 = 0.48711 μM	SANGER
CAL-12T	Growth inhibition assay		IC50 = 0.49125 μM	SANGER
PC-14	Growth inhibition assay		IC50 = 0.49127 μM	SANGER
ST486	Growth inhibition assay		IC50 = 0.49436 μM	SANGER
HLE	Growth inhibition assay		IC50 = 0.49832 μM	SANGER
TE-9	Growth inhibition assay		IC50 = 0.50049 μM	SANGER
AU565	Growth inhibition assay		IC50 = 0.50096 μM	SANGER
LU-165	Growth inhibition assay		IC50 = 0.51311 μM	SANGER
EW-11	Growth inhibition assay		IC50 = 0.51336 μM	SANGER
FTC-133	Growth inhibition assay		IC50 = 0.51722 μM	SANGER
NCI-H1648	Growth inhibition assay		IC50 = 0.52301 μM	SANGER
AN3-CA	Growth inhibition assay		IC50 = 0.52328 μM	SANGER
KURAMOCHI	Growth inhibition assay		IC50 = 0.52344 μM	SANGER
T98G	Growth inhibition assay		IC50 = 0.52375 μM	SANGER
TK10	Growth inhibition assay		IC50 = 0.52832 μM	SANGER
LB2518-MEL	Growth inhibition assay		IC50 = 0.53456 μM	SANGER
CAL-33	Growth inhibition assay		IC50 = 0.53653 μM	SANGER
A172	Growth inhibition assay		IC50 = 0.53752 μM	SANGER
D-263MG	Growth inhibition assay		IC50 = 0.54004 μM	SANGER
CAMA-1	Growth inhibition assay		IC50 = 0.54059 μM	SANGER
MKN1	Growth inhibition assay		IC50 = 0.54081 μM	SANGER
NCI-H661	Growth inhibition assay		IC50 = 0.54158 μM	SANGER
YKG-1	Growth inhibition assay		IC50 = 0.545 μM	SANGER
GOTO	Growth inhibition assay		IC50 = 0.55371 μM	SANGER
KYSE-450	Growth inhibition assay		IC50 = 0.56311 μM	SANGER
UMC-11	Growth inhibition assay		IC50 = 0.56701 μM	SANGER
DBTRG-05MG	Growth inhibition assay		IC50 = 0.57026 μM	SANGER
KYSE-410	Growth inhibition assay		IC50 = 0.58359 μM	SANGER
MOLT-16	Growth inhibition assay		IC50 = 0.58519 μM	SANGER
HCC2218	Growth inhibition assay		IC50 = 0.58882 μM	SANGER
EFO-21	Growth inhibition assay		IC50 = 0.59114 μM	SANGER
S-117	Growth inhibition assay		IC50 = 0.59244 μM	SANGER
HCC1937	Growth inhibition assay		IC50 = 0.59382 μM	SANGER
THP-1	Growth inhibition assay		IC50 = 0.5955 μM	SANGER
GAK	Growth inhibition assay		IC50 = 0.59624 μM	SANGER
SW756	Growth inhibition assay		IC50 = 0.59691 μM	SANGER
KG-1	Growth inhibition assay		IC50 = 0.59714 μM	SANGER
D-542MG	Growth inhibition assay		IC50 = 0.60165 μM	SANGER
HCC38	Growth inhibition assay		IC50 = 0.60411 μM	SANGER
SAS	Growth inhibition assay		IC50 = 0.61465 μM	SANGER
RVH-421	Growth inhibition assay		IC50 = 0.62917 μM	SANGER
JiyoyeP-2003	Growth inhibition assay		IC50 = 0.62954 μM	SANGER
G-401	Growth inhibition assay		IC50 = 0.63447 μM	SANGER
SW1990	Growth inhibition assay		IC50 = 0.63705 μM	SANGER
KP-N-YS	Growth inhibition assay		IC50 = 0.64012 μM	SANGER
HD-MY-Z	Growth inhibition assay		IC50 = 0.64025 μM	SANGER
LAMA-84	Growth inhibition assay		IC50 = 0.64166 μM	SANGER
SW13	Growth inhibition assay		IC50 = 0.64249 μM	SANGER
HCC1569	Growth inhibition assay		IC50 = 0.64461 μM	SANGER
CP66-MEL	Growth inhibition assay		IC50 = 0.64495 μM	SANGER
SK-HEP-1	Growth inhibition assay		IC50 = 0.64566 μM	SANGER
NB13	Growth inhibition assay		IC50 = 0.64603 μM	SANGER
NY	Growth inhibition assay		IC50 = 0.65739 μM	SANGER
EHEB	Growth inhibition assay		IC50 = 0.67213 μM	SANGER
MEG-01	Growth inhibition assay		IC50 = 0.68336 μM	SANGER
HCC70	Growth inhibition assay		IC50 = 0.69433 μM	SANGER
OVCAR-4	Growth inhibition assay		IC50 = 0.69691 μM	SANGER
NCI-H1792	Growth inhibition assay		IC50 = 0.69734 μM	SANGER
HEC-1	Growth inhibition assay		IC50 = 0.69824 μM	SANGER
NCI-H187	Growth inhibition assay		IC50 = 0.70799 μM	SANGER
TGBC24TKB	Growth inhibition assay		IC50 = 0.71 μM	SANGER
HuP-T3	Growth inhibition assay		IC50 = 0.71055 μM	SANGER
SK-MEL-3	Growth inhibition assay		IC50 = 0.71112 μM	SANGER
MDA-MB-415	Growth inhibition assay		IC50 = 0.72395 μM	SANGER
HCC2157	Growth inhibition assay		IC50 = 0.72685 μM	SANGER
NCI-H2081	Growth inhibition assay		IC50 = 0.74057 μM	SANGER
ES6	Growth inhibition assay		IC50 = 0.74562 μM	SANGER
KYSE-520	Growth inhibition assay		IC50 = 0.77218 μM	SANGER
BxPC-3	Growth inhibition assay		IC50 = 0.77478 μM	SANGER
NCI-H226	Growth inhibition assay		IC50 = 0.77721 μM	SANGER
LB771-HNC	Growth inhibition assay		IC50 = 0.77974 μM	SANGER
CP50-MEL-B	Growth inhibition assay		IC50 = 0.78003 μM	SANGER
HN	Growth inhibition assay		IC50 = 0.78254 μM	SANGER
EW-24	Growth inhibition assay		IC50 = 0.78297 μM	SANGER
A673	Growth inhibition assay		IC50 = 0.7896 μM	SANGER
COLO-684	Growth inhibition assay		IC50 = 0.7993 μM	SANGER
DMS-53	Growth inhibition assay		IC50 = 0.80856 μM	SANGER
MDA-MB-361	Growth inhibition assay		IC50 = 0.80898 μM	SANGER
SNU-475	Growth inhibition assay		IC50 = 0.81314 μM	SANGER
LB831-BLC	Growth inhibition assay		IC50 = 0.81608 μM	SANGER
RCM-1	Growth inhibition assay		IC50 = 0.84728 μM	SANGER
NCI-H2347	Growth inhibition assay		IC50 = 0.84997 μM	SANGER
NCI-H2141	Growth inhibition assay		IC50 = 0.85281 μM	SANGER
Becker	Growth inhibition assay		IC50 = 0.86232 μM	SANGER
LC-1F	Growth inhibition assay		IC50 = 0.86233 μM	SANGER
SCC-15	Growth inhibition assay		IC50 = 0.86386 μM	SANGER
U-266	Growth inhibition assay		IC50 = 0.86618 μM	SANGER
SCC-25	Growth inhibition assay		IC50 = 0.87036 μM	SANGER
NCI-H1155	Growth inhibition assay		IC50 = 0.87336 μM	SANGER
NCI-H292	Growth inhibition assay		IC50 = 0.88441 μM	SANGER
NCI-H2196	Growth inhibition assay		IC50 = 0.886 μM	SANGER
EKVX	Growth inhibition assay		IC50 = 0.893 μM	SANGER
TE-6	Growth inhibition assay		IC50 = 0.89367 μM	SANGER
NCI-H1693	Growth inhibition assay		IC50 = 0.89963 μM	SANGER
HuCCT1	Growth inhibition assay		IC50 = 0.90351 μM	SANGER
NCI-H1581	Growth inhibition assay		IC50 = 0.90674 μM	SANGER
KARPAS-422	Growth inhibition assay		IC50 = 0.90739 μM	SANGER
C8166	Growth inhibition assay		IC50 = 0.93232 μM	SANGER
BEN	Growth inhibition assay		IC50 = 0.93516 μM	SANGER
SCLC-21H	Growth inhibition assay		IC50 = 0.96303 μM	SANGER
HT-1376	Growth inhibition assay		IC50 = 0.96711 μM	SANGER
NCI-H1963	Growth inhibition assay		IC50 = 0.97437 μM	SANGER
MPP-89	Growth inhibition assay		IC50 = 0.99122 μM	SANGER
SW1417	Growth inhibition assay		IC50 = 1.00545 μM	SANGER
NCCIT	Growth inhibition assay		IC50 = 1.01024 μM	SANGER
MMAC-SF	Growth inhibition assay		IC50 = 1.01163 μM	SANGER
LU-65	Growth inhibition assay		IC50 = 1.01247 μM	SANGER
NCI-H1623	Growth inhibition assay		IC50 = 1.01428 μM	SANGER
HCT-116	Growth inhibition assay		IC50 = 1.01502 μM	SANGER
CA46	Growth inhibition assay		IC50 = 1.0198 μM	SANGER
RERF-LC-MS	Growth inhibition assay		IC50 = 1.03157 μM	SANGER
DJM-1	Growth inhibition assay		IC50 = 1.03304 μM	SANGER
EM-2	Growth inhibition assay		IC50 = 1.03374 μM	SANGER
HCC1395	Growth inhibition assay		IC50 = 1.05754 μM	SANGER
HCC1143	Growth inhibition assay		IC50 = 1.0664 μM	SANGER
HH	Growth inhibition assay		IC50 = 1.0738 μM	SANGER
MDA-MB-453	Growth inhibition assay		IC50 = 1.07917 μM	SANGER
COLO-320-HSR	Growth inhibition assay		IC50 = 1.0792 μM	SANGER
KM12	Growth inhibition assay		IC50 = 1.08364 μM	SANGER
KNS-42	Growth inhibition assay		IC50 = 1.09239 μM	SANGER
HPAF-II	Growth inhibition assay		IC50 = 1.09715 μM	SANGER
OVCAR-3	Growth inhibition assay		IC50 = 1.12787 μM	SANGER
EFO-27	Growth inhibition assay		IC50 = 1.13122 μM	SANGER
L-428	Growth inhibition assay		IC50 = 1.15778 μM	SANGER
LS-513	Growth inhibition assay		IC50 = 1.17808 μM	SANGER
NB12	Growth inhibition assay		IC50 = 1.20371 μM	SANGER
NCI-H23	Growth inhibition assay		IC50 = 1.20661 μM	SANGER
TGBC1TKB	Growth inhibition assay		IC50 = 1.21141 μM	SANGER
LU-139	Growth inhibition assay		IC50 = 1.21841 μM	SANGER
Raji	Growth inhibition assay		IC50 = 1.22094 μM	SANGER
IPC-298	Growth inhibition assay		IC50 = 1.22841 μM	SANGER
NCI-H838	Growth inhibition assay		IC50 = 1.23291 μM	SANGER
RPMI-8226	Growth inhibition assay		IC50 = 1.23847 μM	SANGER
LC4-1	Growth inhibition assay		IC50 = 1.23959 μM	SANGER
MN-60	Growth inhibition assay		IC50 = 1.26332 μM	SANGER
U-87-MG	Growth inhibition assay		IC50 = 1.27372 μM	SANGER
BL-70	Growth inhibition assay		IC50 = 1.27667 μM	SANGER
MLMA	Growth inhibition assay		IC50 = 1.30978 μM	SANGER
RPMI-8866	Growth inhibition assay		IC50 = 1.31777 μM	SANGER
M14	Growth inhibition assay		IC50 = 1.3376 μM	SANGER
RCC10RGB	Growth inhibition assay		IC50 = 1.34936 μM	SANGER
SK-MEL-24	Growth inhibition assay		IC50 = 1.37423 μM	SANGER
SHP-77	Growth inhibition assay		IC50 = 1.3783 μM	SANGER
EFE-184	Growth inhibition assay		IC50 = 1.38125 μM	SANGER
GI-ME-N	Growth inhibition assay		IC50 = 1.39089 μM	SANGER
SF126	Growth inhibition assay		IC50 = 1.40565 μM	SANGER
Saos-2	Growth inhibition assay		IC50 = 1.40649 μM	SANGER
KARPAS-299	Growth inhibition assay		IC50 = 1.41651 μM	SANGER
MC116	Growth inhibition assay		IC50 = 1.41664 μM	SANGER
NCI-H1563	Growth inhibition assay		IC50 = 1.42784 μM	SANGER
COLO-824	Growth inhibition assay		IC50 = 1.44461 μM	SANGER
IST-SL1	Growth inhibition assay		IC50 = 1.4462 μM	SANGER
HDLM-2	Growth inhibition assay		IC50 = 1.4601 μM	SANGER
NCI-H1573	Growth inhibition assay		IC50 = 1.46085 μM	SANGER
NCI-H2171	Growth inhibition assay		IC50 = 1.49785 μM	SANGER
NOS-1	Growth inhibition assay		IC50 = 1.50227 μM	SANGER
COLO-792	Growth inhibition assay		IC50 = 1.50264 μM	SANGER
SNU-423	Growth inhibition assay		IC50 = 1.52181 μM	SANGER
C32	Growth inhibition assay		IC50 = 1.52834 μM	SANGER
KOSC-2	Growth inhibition assay		IC50 = 1.53103 μM	SANGER
NCI-H64	Growth inhibition assay		IC50 = 1.53444 μM	SANGER
HT	Growth inhibition assay		IC50 = 1.53659 μM	SANGER
NCI-H596	Growth inhibition assay		IC50 = 1.5377 μM	SANGER
NCI-H720	Growth inhibition assay		IC50 = 1.54425 μM	SANGER
UACC-257	Growth inhibition assay		IC50 = 1.56566 μM	SANGER
EVSA-T	Growth inhibition assay		IC50 = 1.57368 μM	SANGER
KNS-81-FD	Growth inhibition assay		IC50 = 1.59446 μM	SANGER
TE-441-T	Growth inhibition assay		IC50 = 1.60276 μM	SANGER
U-698-M	Growth inhibition assay		IC50 = 1.60291 μM	SANGER
COR-L105	Growth inhibition assay		IC50 = 1.61317 μM	SANGER
KMOE-2	Growth inhibition assay		IC50 = 1.62413 μM	SANGER
KY821	Growth inhibition assay		IC50 = 1.65078 μM	SANGER
SJRH30	Growth inhibition assay		IC50 = 1.6742 μM	SANGER
NCI-H69	Growth inhibition assay		IC50 = 1.67882 μM	SANGER
RT4	Growth inhibition assay		IC50 = 1.69923 μM	SANGER
LS-123	Growth inhibition assay		IC50 = 1.72001 μM	SANGER
BHT-101	Growth inhibition assay		IC50 = 1.74029 μM	SANGER
K5	Growth inhibition assay		IC50 = 1.78398 μM	SANGER
SK-N-AS	Growth inhibition assay		IC50 = 1.79073 μM	SANGER
NCI-H322M	Growth inhibition assay		IC50 = 1.80152 μM	SANGER
NCI-H1793	Growth inhibition assay		IC50 = 1.81482 μM	SANGER
CAL-54	Growth inhibition assay		IC50 = 1.84148 μM	SANGER
SK-MEL-1	Growth inhibition assay		IC50 = 1.88815 μM	SANGER
SiHa	Growth inhibition assay		IC50 = 1.8934 μM	SANGER
RD	Growth inhibition assay		IC50 = 1.89846 μM	SANGER
ChaGo-K-1	Growth inhibition assay		IC50 = 1.91033 μM	SANGER
SNU-C1	Growth inhibition assay		IC50 = 1.914 μM	SANGER
NCI-H1436	Growth inhibition assay		IC50 = 1.92742 μM	SANGER
HOP-92	Growth inhibition assay		IC50 = 1.93658 μM	SANGER
HT55	Growth inhibition assay		IC50 = 1.95457 μM	SANGER
K-562	Growth inhibition assay		IC50 = 1.97869 μM	SANGER
NCI-H2052	Growth inhibition assay		IC50 = 1.98812 μM	SANGER
HT-1197	Growth inhibition assay		IC50 = 2.02174 μM	SANGER
SW1463	Growth inhibition assay		IC50 = 2.08169 μM	SANGER
COLO-829	Growth inhibition assay		IC50 = 2.08684 μM	SANGER
DMS-114	Growth inhibition assay		IC50 = 2.08692 μM	SANGER
EFM-19	Growth inhibition assay		IC50 = 2.08896 μM	SANGER
LN-405	Growth inhibition assay		IC50 = 2.15428 μM	SANGER
UACC-62	Growth inhibition assay		IC50 = 2.15533 μM	SANGER
MDA-MB-134-VI	Growth inhibition assay		IC50 = 2.1586 μM	SANGER
MDA-MB-157	Growth inhibition assay		IC50 = 2.19254 μM	SANGER
COLO-741	Growth inhibition assay		IC50 = 2.19928 μM	SANGER
JEG-3	Growth inhibition assay		IC50 = 2.23191 μM	SANGER
NB6	Growth inhibition assay		IC50 = 2.23352 μM	SANGER
NCI-H1395	Growth inhibition assay		IC50 = 2.23483 μM	SANGER
KARPAS-45	Growth inhibition assay		IC50 = 2.27184 μM	SANGER
HCC1954	Growth inhibition assay		IC50 = 2.28119 μM	SANGER
A704	Growth inhibition assay		IC50 = 2.38311 μM	SANGER
SK-N-DZ	Growth inhibition assay		IC50 = 2.38958 μM	SANGER
EGI-1	Growth inhibition assay		IC50 = 2.39059 μM	SANGER
TALL-1	Growth inhibition assay		IC50 = 2.3955 μM	SANGER
PANC-08-13	Growth inhibition assay		IC50 = 2.39777 μM	SANGER
NCI-H28	Growth inhibition assay		IC50 = 2.43524 μM	SANGER
DMS-79	Growth inhibition assay		IC50 = 2.47163 μM	SANGER
NCI-H889	Growth inhibition assay		IC50 = 2.48342 μM	SANGER
Ramos-2G6-4C10	Growth inhibition assay		IC50 = 2.54572 μM	SANGER
BT-474	Growth inhibition assay		IC50 = 2.62326 μM	SANGER
JVM-3	Growth inhibition assay		IC50 = 2.64752 μM	SANGER
NCI-H524	Growth inhibition assay		IC50 = 2.7032 μM	SANGER
SK-MM-2	Growth inhibition assay		IC50 = 2.71695 μM	SANGER
TGW	Growth inhibition assay		IC50 = 2.75803 μM	SANGER
Capan-2	Growth inhibition assay		IC50 = 2.78528 μM	SANGER
NCI-H82	Growth inhibition assay		IC50 = 2.80193 μM	SANGER
NCI-H716	Growth inhibition assay		IC50 = 2.82042 μM	SANGER
MSTO-211H	Growth inhibition assay		IC50 = 2.82369 μM	SANGER
COLO-678	Growth inhibition assay		IC50 = 2.88691 μM	SANGER
AsPC-1	Growth inhibition assay		IC50 = 2.89855 μM	SANGER
DV-90	Growth inhibition assay		IC50 = 2.92143 μM	SANGER
SNU-387	Growth inhibition assay		IC50 = 2.93467 μM	SANGER
KLE	Growth inhibition assay		IC50 = 2.98224 μM	SANGER
U-118-MG	Growth inhibition assay		IC50 = 3.14284 μM	SANGER
MHH-PREB-1	Growth inhibition assay		IC50 = 3.14735 μM	SANGER
MDA-MB-175-VII	Growth inhibition assay		IC50 = 3.15819 μM	SANGER
EB-3	Growth inhibition assay		IC50 = 3.19768 μM	SANGER
HuH-7	Growth inhibition assay		IC50 = 3.22286 μM	SANGER
NCI-H1522	Growth inhibition assay		IC50 = 3.23546 μM	SANGER
MDA-MB-231	Growth inhibition assay		IC50 = 3.23964 μM	SANGER
NCI-H1092	Growth inhibition assay		IC50 = 3.2799 μM	SANGER
KU-19-19	Growth inhibition assay		IC50 = 3.35835 μM	SANGER
SNU-449	Growth inhibition assay		IC50 = 3.36209 μM	SANGER
CAS-1	Growth inhibition assay		IC50 = 3.38185 μM	SANGER
KP-N-YN	Growth inhibition assay		IC50 = 3.41535 μM	SANGER
P31-FUJ	Growth inhibition assay		IC50 = 3.45775 μM	SANGER
RMG-I	Growth inhibition assay		IC50 = 3.46127 μM	SANGER
SN12C	Growth inhibition assay		IC50 = 3.46177 μM	SANGER
HCC1419	Growth inhibition assay		IC50 = 3.48402 μM	SANGER
TT	Growth inhibition assay		IC50 = 3.67775 μM	SANGER
SW948	Growth inhibition assay		IC50 = 3.75281 μM	SANGER
SCC-9	Growth inhibition assay		IC50 = 3.78753 μM	SANGER
KMS-12-PE	Growth inhibition assay		IC50 = 3.82788 μM	SANGER
GMS-10	Growth inhibition assay		IC50 = 3.83807 μM	SANGER
EW-18	Growth inhibition assay		IC50 = 3.9388 μM	SANGER
SK-MEL-2	Growth inhibition assay		IC50 = 3.97464 μM	SANGER
PC-3	Growth inhibition assay		IC50 = 4.07159 μM	SANGER
IST-MES1	Growth inhibition assay		IC50 = 4.14621 μM	SANGER
PLC-PRF-5	Growth inhibition assay		IC50 = 4.19446 μM	SANGER
YAPC	Growth inhibition assay		IC50 = 4.36751 μM	SANGER
RXF393	Growth inhibition assay		IC50 = 4.38975 μM	SANGER
LP-1	Growth inhibition assay		IC50 = 4.55967 μM	SANGER
JAR	Growth inhibition assay		IC50 = 4.56384 μM	SANGER
GCT	Growth inhibition assay		IC50 = 4.6047 μM	SANGER
SW1088	Growth inhibition assay		IC50 = 4.66827 μM	SANGER
NCI-H345	Growth inhibition assay		IC50 = 4.73193 μM	SANGER
Calu-1	Growth inhibition assay		IC50 = 4.8072 μM	SANGER
ECC4	Growth inhibition assay		IC50 = 5.07036 μM	SANGER
DOK	Growth inhibition assay		IC50 = 5.1551 μM	SANGER
RL	Growth inhibition assay		IC50 = 5.43657 μM	SANGER
NCI-H1755	Growth inhibition assay		IC50 = 5.43694 μM	SANGER
HTC-C3	Growth inhibition assay		IC50 = 5.78944 μM	SANGER
PFSK-1	Growth inhibition assay		IC50 = 5.88351 μM	SANGER
CAL-120	Growth inhibition assay		IC50 = 5.96351 μM	SANGER
Hs-578-T	Growth inhibition assay		IC50 = 6.20302 μM	SANGER
DG-75	Growth inhibition assay		IC50 = 6.2494 μM	SANGER
NCI-H2227	Growth inhibition assay		IC50 = 6.32364 μM	SANGER
OPM-2	Growth inhibition assay		IC50 = 6.34857 μM	SANGER
T47D	Growth inhibition assay		IC50 = 6.37191 μM	SANGER
HuO-3N1	Growth inhibition assay		IC50 = 6.45452 μM	SANGER
D-392MG	Growth inhibition assay		IC50 = 6.68451 μM	SANGER
NCI-SNU-16	Growth inhibition assay		IC50 = 6.74309 μM	SANGER
CW-2	Growth inhibition assay		IC50 = 7.72758 μM	SANGER
no-11	Growth inhibition assay		IC50 = 7.74202 μM	SANGER
MFE-280	Growth inhibition assay		IC50 = 8.34873 μM	SANGER
KINGS-1	Growth inhibition assay		IC50 = 8.61164 μM	SANGER
HCC1187	Growth inhibition assay		IC50 = 8.9213 μM	SANGER
NCI-H2452	Growth inhibition assay		IC50 = 8.93628 μM	SANGER
C2BBe1	Growth inhibition assay		IC50 = 9.284 μM	SANGER
Mewo	Growth inhibition assay		IC50 = 9.55233 μM	SANGER
MFM-223	Growth inhibition assay		IC50 = 10.3807 μM	SANGER
NCI-H446	Growth inhibition assay		IC50 = 12.4227 μM	SANGER
RO82-W-1	Growth inhibition assay		IC50 = 13.3274 μM	SANGER
EC-GI-10	Growth inhibition assay		IC50 = 14.9132 μM	SANGER
UACC-812	Growth inhibition assay		IC50 = 16.8306 μM	SANGER
SK-MEL-28	Growth inhibition assay		IC50 = 18.9731 μM	SANGER
DK-MG	Growth inhibition assay		IC50 = 21.667 μM	SANGER
NCI-H1993	Growth inhibition assay		IC50 = 22.984 μM	SANGER
U-2-OS	Growth inhibition assay		IC50 = 23.6987 μM	SANGER
MKN7	Growth inhibition assay		IC50 = 27.8562 μM	SANGER
SK-N-FI	Growth inhibition assay		IC50 = 39.7075 μM	SANGER
CAL-72	Growth inhibition assay		IC50 = 46.7339 μM	SANGER
Click to View More Cell Line Experimental Data

Mechanism of Action

Description

Targets

DNA synthesis ^[1]

In vitro
In vitro	Mitomycin C, by inducing DNA interstrand crosslinks, physically blocks DNA replication, recombination, and RNA transcription. ^[1] Mitomycin C potentiates TRAIL-induced apoptosis in HCT116 (p53-/-) colon cancer cells and sensitizes TRAIL-resistant colon cancer cells HT-29 to the cytokine by through JNK-independent upregulation of death receptors. ^[2] In different human cancer cell lines, such OVCAR-5 (ovary), HT-29 (colon), SK-N-MC (neuroblastoma), HEP-2 (liver), COLO-205 (colon), NIH-OVCAR-3 (ovary) and A-549 (lung) cells, Mitomycin C shows cytotoxic activity. ^[3]
Cell Research	Cell lines	HCT116 cells
	Concentrations	5 µM
	Incubation Time	24 h
	Method	Cells were pre-treated with 5 µM MMC for 24 h and then co-treated with TRAIL (25 ng/mL).
Experimental Result Images	Methods	Biomarkers	Images	PMID
Experimental Result Images	Western blot	GRP78 / CHOP / Caspase-4 / Cleaved caspase-4 Beclin 1 / p62 / ATG5 p-Akt / Akt / p-S6K / S6K / p-Jun / JNK PARP / Caspase 8 / Caspase 9 / Cleaved caspase-9 / Caspase 3 / Cleaved caspase-3		28553347

In Vivo
In vivo	As the clinical first choice in superficial bladder tumors, in a rat bladder tumor model, Mitomycin C (400 μM) significantly prevents intravesical tumor growth. ^[4]
Animal Research	Animal Models	Rat bladder tumor model
	Dosages	400 μM
	Administration	Intravesical treatment

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT04934540	Recruiting	Bladder Cancer	Chinese University of Hong Kong	January 1 2020	--
NCT02199327	Completed	Conjunctival Intraepithelial Neoplasia\|Corneal Intraepithelial Neoplasia	Coordinación de Investigación en Salud Mexico\|Instituto Mexicano del Seguro Social\|University of Guadalajara	May 2014	Phase 4

References
https://pubmed.ncbi.nlm.nih.gov/19748363/ https://pubmed.ncbi.nlm.nih.gov/22895172/ https://pubmed.ncbi.nlm.nih.gov/24791416/ https://pubmed.ncbi.nlm.nih.gov/24220494/ + Expand

References

https://pubmed.ncbi.nlm.nih.gov/19748363/
https://pubmed.ncbi.nlm.nih.gov/22895172/
https://pubmed.ncbi.nlm.nih.gov/24791416/

https://pubmed.ncbi.nlm.nih.gov/24220494/

+ Expand

Chemical Information

Molecular Weight	334.37	Formula	C₁₅H₁₈N₄O₅
CAS No.	50-07-7	SDF	Download Mitomycin C SDF
Synonyms	Ametycine
Smiles	CC1=C(C(=O)C2=C(C1=O)N3CC4C(C3(C2COC(=O)N)OC)N4)N

Storage and Stability

Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 66 mg/mL ( (197.38 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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