Iodoacetamide

Name: Iodoacetamide
Brand: Selleck Chemicals
SKU: S9827
Rating: 5 (2 reviews)

Synonyms: IA, IAM, 2-Iodoacetamide

Catalog No.S9827 Purity:97%

Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

Iodoacetamide Chemical Structure

CAS No. 144-48-9

Purity & Quality Control

Purity: >97%

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Biological Activity

Description	Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

In vitro
In vitro	A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.^[2]
Cell Research	Cell lines	Drosophila melanogaster cells
	Concentrations	2 mM
	Incubation Time	15 mins
	Method	Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.

In Vivo
In vivo	Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.^[3]
Animal Research	Animal Models	Wistar rats, 8-week-old male CD1 and C57Bl/6J mice
	Dosages	0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration
	Administration	Oral gavage

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05986214	Not yet recruiting	Chronic Obstructive Pulmonary Disease	VA Office of Research and Development	March 1 2026	Not Applicable
NCT06376253	Not yet recruiting	Ovarian Cancer\|Lung Cancer	Novartis Pharmaceuticals\|Novartis	September 6 2024	Phase 1
NCT06406309	Not yet recruiting	Attention Deficit Hyperactivity Disorder\|Sleep Disturbance	University of Pittsburgh\|The Klingenstein Third Generation Foundation	September 2024	Not Applicable

References

Chemical Information & Solubility

Molecular Weight	184.96	Formula	C₂H₄INO
CAS No.	144-48-9	SDF	--
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 40 mg/mL ( (216.26 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 40 mg/mL

Ethanol : 40 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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