Iodoacetamide

Catalog No.S9827 Batch:S982701

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Technical Data

Formula

C2H4INO
Molecular Weight 184.96 CAS No. 144-48-9
Solubility (25°C)* In vitro DMSO 40 mg/mL (216.26 mM)
Water 40 mg/mL (216.26 mM)
Ethanol 40 mg/mL (216.26 mM)
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
In vitro

A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2]
In vivo

Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3]

Protocol (from reference)

Cell Assay:

[2]
  • Cell lines

    Drosophila melanogaster cells

  • Concentrations

    2 mM

  • Incubation Time

    15 mins

  • Method

    Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.
Animal Study:

[3]
  • Animal Models

    Wistar rats, 8-week-old male CD1 and C57Bl/6J mice

  • Dosages

    0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration

  • Administration

    Oral gavage

Selleck's Iodoacetamide has been cited by 2 publications

The compound millepachine and its derivatives inhibit tubulin polymerization by irreversibly binding to the colchicine-binding site in β-tubulin [ J Biol Chem, 2018, 293(24):9461-9472] PubMed: 29691282
Global MS-Based Proteomics Drug Profiling [ Methods Mol Biol, 2016, 1449:469-79] PubMed: 27613057

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.