Home Immunology & Inflammation Histamine Receptor agonist Histamine For research use only.

Histamine

Histamine, an organic nitrogenous compound, is involved in local immune responses regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is a potent H1 and H2 receptor agonist.

Histamine Chemical Structure

Histamine Chemical Structure

CAS No. 51-45-6

Purity & Quality Control

Histamine Related Products

Signaling Pathway

Histamine Receptor Signaling Pathways

Histamine Receptor Signaling Pathway

Biological Activity

Description Histamine, an organic nitrogenous compound, is involved in local immune responses regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus. It is a potent H1 and H2 receptor agonist.
In vitro
In vitro Histamine stimulates the proliferation of human articular chondrocytes in culture. Histamine is reported to modify the behaviour of many cell types in vitro, including chondrocytes, fibroblasts, macrophages, epithelial cells, endothelial cells, and T cells. Histamine also modulates the production of many cytokines and the expression of their receptors. Histamine regulates cellular processes through the expression of histamine receptors. Histamine stimulates the production of matrix metalloproteinases (MMPs)-13 and -3 (collagenase 3 and stromelysin-1, respectively) by histidine decarboxylase (HAC) in vitro[1].
Cell Research Cell lines human articular chondrocytes
Concentrations 1-100 μmol/l
Incubation Time 6 days
Method Cells are grown to confluence in 80 cm2 culture flasks in Dulbecco's modified Eagle's medium (DMEM) + 10% fetal calf serum (FCS). At first or second passage, cells are seeded into 96 well culture plates, at densities of approximately 2×103 cells/well. After 24 hours the cells are treated with either DMEM +2% FCS alone (control), or DMEM + 2% FCS plus histamine range 1-100 μmol/l (minimum of eight wells for each treatment). The medium with or without histamine is replenished every 48 hours, and after six days cell growth is estimated.
In Vivo
In vivo Histamine has a recognised role in allergic and inflammatory reactions and is an important modulator of numerous physiological processes, including cell proliferation, angiogenesis, and vasopermeability[1]. Histamine dilates vasculature, increases blood flow, while it induces hyperpermeability in venula. It disrupts endothelial barrier formation of venula indicated by changes in vascular endothelial cadherin (VE-cadherin) localization at endothelial cell junction[3].
Animal Research Animal Models New Zealand adult healthy albino rabbits
Dosages 50, 100, 200 μg/kg
Administration s.c.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06281366 Recruiting
Pregnancy Complications
Hospital Universitari Vall d''Hebron Research Institute|DR Healthcare
 February 16 2024 --
NCT05216133 Enrolling by invitation
Airway Disease|Barrett Esophagus|Gastroesophageal Reflux Disease
NYU Langone Health|National Institute for Occupational Safety and Health (NIOSH/CDC)
 March 22 2023 --
NCT05701826 Completed
Skeletal Muscle Relaxation
Fujian Shengdi Pharmaceutical Co. Ltd.
 February 15 2023 Phase 1
NCT05676346 Completed
Lower Urinary Tract Symptoms|Histamine Intolerance
Complexo Hospitalario Universitario de A Coruña
 October 11 2022 --

Chemical Information & Solubility

Molecular Weight 111.15 Formula

C5H9N3
CAS No. 51-45-6 SDF Download Histamine SDF
Smiles C1=C(NC=N1)CCN
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 22 mg/mL ( (197.93 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 22 mg/mL

Ethanol : 22 mg/mL


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